Browsing by Autor "Abigail Condori-Mamani"

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Assessment of the drinking water quality in the municipality of Vinto-Cochabamba-Bolivia
(2025) Abdiel Adriazola Muriel; Vanessa Nancy Toro Coca; Abigail Condori-Mamani; Raquel Orellana Guevara; Moria Villca Chuquichambi
<p class="MsoNormal" style="text-align: justify; text-justify: inter-ideograph; line-height: 120%; mso-pagination: none; layout-grid-mode: char; mso-layout-grid-align: none; punctuation-wrap: simple; text-autospace: none; margin: 12.0pt 0cm 6.0pt 0cm;">Since coliform bacteria can raise the risk of infectious diseases in the most susceptible groups, their presence in water poses a possible concern to public health. When the physicochemical and microbiological quality of the water is uncertain, the situation becomes more complex. This study aims to assess the drinking water quality in three Territorial Base Organizations (TBOs) of the Municipality of Vinto-Cochabamba as well as the link between the TBOs and the microbiological parameters. According to Bolivian Standard 512 (NB 512), laboratory analyses of 30 samples collected from various residences (10 samples per TBO) demonstrate compliance with the upper limits of allowable values for physicochemical parameters (pH, conductivity, alkalinity, and hardness). The microbiological parameters were subjected to chi-square analysis, showing enough evidence to confirm with 95% confidence that the results of total and heat-resistant coliforms do not depend on the TBO (p = 0.36). By comparing the results of household water consumption with those of water supplied, it can be hypothesized that contamination of microbiological origin occurs either in the distribution pipes, household tanks, and/or the water use practices of each household. Of the 30 samples analyzed, only 11 met the maximum permissible limit for total coliforms, whereas 25 of the 30 samples met the maximum permissible limit for total coliforms.
Comparación entre el método de hemaglutinación indirecta y ELISA para la detección de anticuerpos IgG contra Toxoplasma gondii
(2025) Moria Villca Chuquichambi; Abigail Condori-Mamani; Raquel Orellana Guevara; Jhanneth Mamani-Garabito; Juan Wilfredo Choque Medrano
Introduction: Toxoplasmosis, caused by Toxoplasma gondii, is an infection that can have serious consequences, especially in pregnant women. Objetives: Compare the indirect hemagglutination method (HAI) and ELISA for the detection of IgG antibodies against Toxoplasma gondii, evaluating their sensitivity, specificity, and concordance. Methods: A total of 84 sera from patients with suspected toxoplasmosis were analyzed. All sera were tested using the indirect hemagglutination assay (IHA) and ELISA as a reference test. The sensitivity and specificity of IHA as well as the concordance between the two diagnostic methods were determined. On the other hand, Pearson’s Chi-square test was applied, considering a p-value below 0.05 as statistically significant. Results: The IHA method yielded 76.2% positive and 23,8 % negative results. whereas, the ELISA method showed 77,4 % positive and 22,6 % negative results. A strong agreement and a statistically significant correlation (p=0.001) were observed between both methods. The IHA method demonstrated a sensitivity of 98.56% and a specificity of 100.00%. Conclusions: The comparison between the indirect hemagglutination (HAI) method and ELISA for the detection of IgG antibodies against Toxoplasma gondii indicates that both methods exhibit similar effectiveness in identifying positive and negative cases. Despite differences in the procedures, both methods are valid and reliable tools for the serological diagnosis of toxoplasmosis.
Evaluación de prueba inmunocromatográfica para la detección de anticuerpos IgM e IgG contra SARS-CoV-2
(2023) Moria Villca Chuquichambi; Raquel Orellana Guevara; Abigail Condori-Mamani; Juan Wilfredo Choque Medrano
Introducción: Las pruebas inmunológicas son utilizadas como diagnóstico complementario en la vigilancia epidemiológica. Objetivos: Evaluar la prueba inmunocromatográfica para la detección de anticuerpos IgM e IgG contra el SARS-CoV-2, usando como prueba de referencia ELISA IgM/IgG para COVID-19. Metodología: El estudio realizado comparó pruebas inmunocromatográficas IgM/IgG con la prueba de ELISA IgM/IgG. También se efectuó la estimación de sensibilidad y especificidad de dicha prueba. Se trabajó con 50 muestras de suero para IgM y 50 muestra para IgG. Resultados: La sensibilidad de la prueba Inmunocromatográfica evaluada para la detección de anticuerpos IgM fue de 78,95 % y una especificidad de 48,39 %. En cuanto a la prueba inmunocromatográfica para la detección de anticuerpos, IgG fue de 72,00 % de sensibilidad y especificidad de 76,00 %. Discusión: La concordancia entre la prueba inmunocromatográfica para la detección de anticuerpos IgM/IgG y la prueba de ELISA para la detección de anticuerpos IgM/IgG muestra una concordancia regular y Moderada según el Índice kappa. La evaluación de pruebas inmunocromatográficas presenta una sensibilidad y especificidad menor frente a otras pruebas.
Frecuencia y conocimiento de tripanosomiasis americana en habitantes de la municipalidad de Vinto, Cochabamba, Bolivia
(2024) Moria Villca Chuquichambi; Abigail Condori-Mamani; Raquel Orellana Guevara; Javier Orellana-Salazar
Introduction: American trypanosomiasis is caused by the blood parasite Trypanosoma cruzi, it affects 21 countries in the America, especially rural populations. Objectives: To determine the frequency and knowledge of American trypanosomiasis, as well as to disaggregate the frequency of American trypanosomiasis reemplazar por by sex and age group in the inhabitants of the municipality of Vinto, Cochabamba - Bolivia. Materials and Methods: A descriptive study with a non-experimental cross-sectional design was carried out. A total of 207 inhabitants of the municipality of Vinto attended the clinical analysis laboratory of the Biochemistry Department of the Adventist University of Bolivia. The sera were processed by indirect hemagglutination (HAI). Knowledge about American trypanosomiasis was determined by means of a structured survey. Pearson’s chi-square was used to relate variables and was considered significant at p<0.05. Results: The frequency of American trypanosomiasis in inhabitants of the municipality of Vinto was 11.6%. There was no relationship between the frequency of American trypanosomiasis and sex (p>0.05) but there was a relationship with the age group (p<0.05). 72.9% claimed to know about American trypanosomiasis. Conclusions: The results show that the frequency of American trypanosomiasis is low and that most of the inhabitants of the municipality of Vinto, Cochabamba, Bolivia have knowledge about the disease. In addition, it was found that there is only a relationship betweenthe frequency of American trypanosomiasis and age group.