Browsing by Autor "Aldo Solari"

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An isoenzyme study of naturally occurring clones of Trypanosoma cruzi isolated from both sides of the West Andes highland
(Oxford University Press, 1991) Simone Frédérique Brénière; Paul Braquemond; Aldo Solari; Jean‐François Agnèse; Michel Tibayrenc
Seventy-two stocks of Trypanosoma cruzi isolated from both sides of the West Andes highland (Bolivia, Chile and Peru) were analysed by isoenzyme electrophoresis at 12 loci. The data, which were interpreted in terms of population and evolutionary genetics, corroborated the hypothesis of T. cruzi clonal population structure previously proposed, and indicated extensive genetic variability within the taxon T. cruzi. Fifteen different clones (or zymodemes) were identified, which could be grouped into 3 different clusters. Several clones from 2 of these clusters were isolated both in Chile and Bolivia, suggesting a significant circulation of invertebrate and/or vertebrate hosts of T. cruzi between these 2 countries. Low clonal variability in Peru suggested the occurrence of a 'founder effect' in this country. The potential usefulness of a cladistic approach in epidemiology is discussed.
Phylogenetic Analysis of Bolivian Bat Trypanosomes of the Subgenus Schizotrypanum Based on Cytochrome b Sequence and Minicircle Analyses
(Public Library of Science, 2012) Lineth García; Sylvia Ortiz; Gonzalo Osorío; Mary Cruz Torrico; Faustino Torrico; Aldo Solari
The aim of this study was to establish the phylogenetic relationships of trypanosomes present in blood samples of Bolivian Carollia bats. Eighteen cloned stocks were isolated from 115 bats belonging to Carollia perspicillata (Phyllostomidae) from three Amazonian areas of the Chapare Province of Bolivia and studied by xenodiagnosis using the vectors Rhodnius robustus and Triatoma infestans (Trypanosoma cruzi marenkellei) or haemoculture (Trypanosoma dionisii). The PCR DNA amplified was analyzed by nucleotide sequences of maxicircles encoding cytochrome b and by means of the molecular size of hyper variable regions of minicircles. Ten samples were classified as Trypanosoma cruzi marinkellei and 8 samples as Trypanosoma dionisii. The two species have a different molecular size profile with respect to the amplified regions of minicircles and also with respect to Trypanosoma cruzi and Trypanosoma rangeli used for comparative purpose. We conclude the presence of two species of bat trypanosomes in these samples, which can clearly be identified by the methods used in this study. The presence of these trypanosomes in Amazonian bats is discussed.
The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
(Public Library of Science, 2014) Koen De Winne; Philippe Büscher; Alejandro O. Luquetti; Suelene B. N. Tavares; Rodrigo Arruda de Oliveira; Aldo Solari; Inés Zulantay; Werner Apt; Patricio Diosque; Mercedes Rumi
Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p = 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.