Browsing by Autor "Amilcar Flores León"

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    Cuantificación de niveles de INF-y - IL-13 y células T CD4+, CD8+ en pacientes con leishmaniasis tegumentaria con falla terapéutica
    (2013) Amilcar Flores León; Ernesto Rojas Cabrera; Marisol Córdova Rojas; Mary Cruz Torrico; David Paz
    Objetivos: se realiza un estudio del estatus inmunologico, cuantificando el perfil de citoquinas Th1 (INF-γ) y Th2 (IL-13) y celulas T CD3+, CD4+ y CD8+ como variables que puedan brindar informacion sobre el vinculo y/o asociacion a la falla terapeutica. Metodos: se cuantifico los niveles de INF-γ , IL-13, CD4 + y CD8+ en tres grupos de estudio: a) Pacientes con LT y falla terapeutica (RESISTENTES), b) Pacientes tratados que respondieron al tratamiento de forma exitosa (SENSIBLES) c) Pacientes sanos (GRUPO CONTROL). Resultados: los resultados indican, que la respuesta especifica inmune de los pacientes Resistentes y Sensibles esta polarizada hacia la respuesta TH1 y los valores de los Linfocitos T CD4+ y CD8+ estaban por debajo de los valores normales en los tres grupos de estudio. Los niveles de produccion de INF- γ fue mayor que la IL-13, siendo mas pronunciada en pacientes Resistentes que Sensibles en respuesta al Ag de Leishmania, la tipificacion de las cepas que fueron aisladas de los pacientes resistentes, identificaron a: Leishmania brasiliensis y Leishmania guayanensis. Conclusiones: en relacion a la falla terapeutica de los pacientes resistentes, estarian tambien involucrados los factores relacionados al parasito y otros factores extrinsecos al huesped. Palabras claves: leishmaniasis cutanea; fracaso del tratamiento; citocinas; recuento de linfocito CD4; interferones. Objectives: is a study of the immune status, quantifying the Th1 cytokine profile (IFN-γ) and Th2 (IL-13) and CD3 + T cells, CD4 + and CD8 + as variables that can provide information on the link and / or association therapeutic failure. Methods: we quantified the levels of INF-γ, IL-13, CD4 + and CD8 + in three study groups: a) patients with LT and therapeutic failure (RESISTANT), b) Treated patients who responded to treatment successfully (SENSITIVE ) c) healthy patients (CONTROL GROUP). Results: the results indicate that the specific immune res ponse of resistant and sensitive patients is polarized toward the TH1 response and values of CD4 + T lymphocytes and CD8 + were below normal values in the thr ee groups. The levels of IFN-γ production was higher than IL-13, being more pronounced in patients resistant to sensitive in response to Leishmania Ag, the typing of the strains that were isolated from patients resistant identified: Leishmania brasi liensis and guayanensis Leishmania. Conclusions: regarding treatment failure resistant patients, would also be involved factors related to the parasite and other extrinsic factors to the host.
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    Rol de anticuerpos anti- Toxoplasma gondii en la activación de la respuesta inmune en mujeres embarazadas.
    (2013) Amilcar Flores León; Norman Rojas; Karina Mamani Herrer; Emilse Egüez Suarez
    Objectives: the study aimed to evaluate in vitro the role of anti T. gondiiin activating immune responses in pregnant women. Methods: the study was performed with peripheral blood mononuclear cells (PBMC) of pregnant women with chronic toxoplasmosis (n = 15) were stimulated in the presence and absence of autologous plasma (PA) (anti T. gondii antibody). Results: the data show that in PBMC stimu- lated in the absence of autologous plasma there is increased cell proliferation (P < 0.05) than cells in the presence of autologous plasma. Levels of IFN- γ produced in both conditions (PA and SBF) were similar. Comparing the production of IFN- γ vs IL - 10 shows increased production of Th1 cytokines. Conclusions: in general, our results suggest that the antibodies present in autologous plasma modulate the immune response in pregnant women with chronic toxoplasmosis, such that the immune system does not exacerbate or inhibit this specific response. The presence of antibodies to T. gondii not affecting IFN- γ production in pregnant women with chronic toxoplasmosis, but if cell proliferation.