Browsing by Autor "Angel Barrios"

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Antiprotozoal activity of quinoline alkaloids isolated from <i>Galipea longiflora</i>, a Bolivian plant used as a treatment for cutaneous leishmaniasis
(Wiley, 1994) Alain Fournet; Angel Barrios; Verónica Francisca Loewe Muñoz; Reynald Hocquemiller; F. Roblot; A. Cavé; Pascal Richomme; J. Bruneton
Abstract The stem bark of Galipea longiflora is used by the Chimane Indians in Bolivia for the treatment of cutaneous leishmaniasis produced by Leishmania braziliensis . Petroleum ether and chloroform extracts of stem, root bark and leaves were found active in vitro against Leishmania ssp and Trypanosoma cruzi at 100 μg/mL. The activity guided fractionation of the extracts by chromatography afforded 12 active compounds identified as 2‐substituted quinoline alkaloids. BALB/c mice were infected with Leishmania amazonensis (strain PH8 or H‐142) and treated 24 h after infection with the major alkaloids from the crude alkaloidal extract; 2‐phenylquinoline and 2‐n‐pentylquinoline. 2‐phenylquinoline was as potent as Glucantime (Rhǒne‐Poulenc) against the strain H‐142, but less active than the reference drug against the virulent strain PH8 of L. amazonensis. 2‐n ‐pentylquinoline did not exhibit any activity. Assays of single local treatments on the rear footpad infection, 2 weeks after the parasitic inoculation, indicated an effect for 2‐phenylquinoline by reducing the severity of lesion. However, this activity was found to be slightly lower than that obtained using Glucantime.
Effect of some bisbenzylisoquinoline alkaloids on American <i>Leishmania</i> sp. in BALB/c mice
(Wiley, 1993) Alain Fournet; Angel Barrios; Victoria Muñoz; Reynald Hocquemiller; André Cavé
Abstract Four bisbenzylisoquinoline alkaloids, antioquine, berbamine, gyrocarpine and isotetrandrine were tested in BALB/c mice infected with Leishmania amazonensis (IFLA/BR/67/PH8 or MHOM/GF/84/CAY‐H‐142) or L. venezuelensis (VE/74/PM‐H3). The treatments were initiated 1 day after the parasitic infection, with alkaloid at 100 mg/kg/day for 14 days and the reference compound, meglumine antimonate (Glucantime R ) at 200 mg/kg/day. Antioquine, berbamine and gyrocarpine were less potent than Glucantime against L. amazonensis (PH8). Only isotetrandrine exhibited activity approximately equal to or greater than Glucantime in BALB/c mice infected with L. amazonensis (PH8 or H‐142) and showed significant activity against L. venezuelensis . Experiments with a single local treatment on the footpad, 2 weeks after parasitic infection with L. amazonensis (PH8), showed that isotetrandrine at 200 mg/kg was less active than Glucantime at 400 mg/kg.
Leishmanicidal and trypanocidal activities of Bolivian medicinal plants
(Elsevier BV, 1994) Alain Fournet; Angel Barrios; Victoria Muñoz