Browsing by Autor "Anselmo Gil-Rocha"

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Natural Biocontrol of Athelia rolfsii isolate INVEPAR-05 in Stevia rebaudiana Bertoni: Exploring the Biocontrol Potential of Native Trichoderma spp. Strains
(2023) Luis Alfonso Rodríguéz-Páez; Juán de Diós Jaraba-Navas; Yirlis Yadeth Pineda‐Rodríguez; Mauricio Begambre-Hernandez; Marcelo F. Pompelli; Ana Melisa Jimenez-Ramirez; Anselmo Gil-Rocha; Alfredo Jarma‐Orozco; Enrique Combatt Caballero; Katia Aviña‐Padilla
Stevia rebaudiana (Bert.) is a natural sweetener with diterpenic glycosides, offering 300 times more sweetness than sucrose, and boasting low-calorie content, along with medicinal and therapeutic properties. To address a limited genetic diversity, a germplasm bank comprising 115 segregated lines of stevia was established in Colombia. Notwithstanding, the fungus Athelia rolfsii adversely impacted key stevia lines, leading to wilting and plant mortality. For this reason our study aimed to explore the biocontrol potential of native strains of Trichoderma spp., isolated from productive lots in Colombia's Cordoba department, as a solution to manage A. rolfsii. Soil samples underwent serial dilution technique (103 conidia.mL-1) to obtain Trichoderma spp. isolates, which were thoroughly morphologically and molecularly characterized. A total five native strains were identified through sequencing the PCR products using universal ITS primers, and comparisons with the GENBANK database confirmed their identity (OK310695.1, ON237703.1, ON238106.1, ON237737.1, and ON237923.1). Their biocontrol efficacy against A. rolfsii was evaluated using the dual culture technique. Remarkably, all strains exhibited significant biocontrol capacity against the A. rolfsii isolate INVEPAR-05 (OK271308.1), except for Trichoderma viride INVEPAR-T10 (ON237923.1). These findings suggest that Trichoderma spp. could be a potential option to manage the fungus in stevia.