Browsing by Autor "Brigitte Bastrenta"

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Different Behavior of TwoTrypanosoma cruziMajor Clones: Transmission and Circulation in Young Bolivian Patients
(Elsevier BV, 1998) Simone Frédérique Brénière; Marie-France Bosseno; Jenny Telleria; Brigitte Bastrenta; Nina Yacsik; François Noireau; Jose-Luis Alcazar; Christian Barnabé; Patrick Wincker; Michel Tibayrenc
Experimental Trypanosoma cruzi biclonal infection in Triatoma infestans: detection of distinct clonal genotypes using kinetoplast DNA probes
(Elsevier BV, 2000) Artur da Silveira Pinto; Marta de Lana; Constança Britto; Brigitte Bastrenta; Michel Tibayrenc
First evidence of transmission of Leishmania (Viannia) lainsoni in a Sub Andean region of Bolivia
(Elsevier BV, 2002) Brigitte Bastrenta; R Buitrago; Fernando Regla Vargas; François Le Pont; M Torrez; María Flóres-Chávez; N Mita; Simone Frédérique Brénière
Human mixed infections of Leishmania spp. and Leishmania-Trypanosoma cruzi in a sub Andean Bolivian area: identification by polymerase chain reaction/hybridization and isoenzyme
(Instituto Oswaldo Cruz, Ministério da Saúde, 2003) Brigitte Bastrenta; N Mita; R Buitrago; Fernando Regla Vargas; María Flóres-Chávez; M Machane; Nina Yacsik; M Torrez; François Le Pont; Simone Frédérique Brénière
Parasites belonging to Leishmania braziliensis, Leishmania donovani, Leishmania mexicana complexes and Trypanosoma cruzi (clones 20 and 39) were searched in blood, lesions and strains collected from 28 patients with active cutaneous leishmaniasis and one patient with visceral leishmaniasis. PCR-hybridization with specific probes of Leishmania complexes (L. braziliensis, L. donovani and L. mexicana) and T. cruzi clones was applied to the different DNA samples. Over 29 patients, 8 (27.6%) presented a mixed infection Leishmania complex species, 17 (58.6%) a mixed infection Leishmania-T. cruzi, and 4 (13.8%) a multi Leishmania-T. cruzi infection. Several patients were infected by the two Bolivian major clones 20 and 39 of T. cruzi (44.8%). The L. braziliensis complex was more frequently detected in lesions than in blood and a reverse result was observed for L. mexicana complex. The polymerase chain reaction-hybridization design offers new arguments supporting the idea of an underestimated rate of visceral leishmanisis in Bolivia. Parasites were isolated by culture from the blood of two patients and lesions of 10 patients. The UPGMA (unweighted pair-group method with arithmetic averages) dendrogram computed from Jaccard's distances obtained from 11 isoenzyme loci data confirmed the presence of the three Leishmania complexes and undoubtedly identified human infections by L. (V.) braziliensis, L. (L.) chagasi and L. (L.) mexicana species. Additional evidence of parasite mixtures was visualized through mixed isoenzyme profiles, L. (V.) braziliensis-L. (L.) mexicana and Leishmania spp.-T. cruzi. The epidemiological profile in the studied area appeared more complex than currently known. This is the first report of parasitological evidence of Bolivian patients with trypanosomatidae multi infections and consequences on the diseases' control and patient treatments are discussed.
Is <i>Rhodnius robustus</i> (Hemiptera: Reduviidae) responsible for Chagas disease transmission in Western Venezuela?
(Wiley, 2002) M. Dora Feliciangeli; Jean‐Pierre Dujardin; Brigitte Bastrenta; Milena Mazzarri; Judith Villegas; María Flóres-Chávez; Maruska Muñoz
We present evidence for the putative role of Rhodnius robustus as extradomestic vector of Chagas disease in Western Venezuela. First, we assessed the validity of this triatomine species by genetic characterization in relation with some other species of the prolixus group. Random amplified polymorphic DNA data showed a clear separation between this species and R. prolixus and indicated a probable genetic heterogeneity within R. robustus. Faeces and gut contents were microscopically examined in 54 of 137 R. robustus collected in palm trees. According to this morphological examination, 18% were positive for Trypanosoma cruzi, 11% harboured T. rangeli and 11% showed mixed infection. Five of the seven samples examined gave a polymerase chain reaction major band of 270 bp specific of T. cruzi. The hybridization probes showed that R. robustus may transmit clones 20 and 39 (or genetically related ones) in Venezuela. Such a transmission might occur when, in absence of domestic R. prolixus and attracted by artificial light, R. robustus enters houses and feeds on humans, or when people are bitten outdoors. The lack of bugs inside houses could mean that the insects leave houses after feeding, or die without reproducing there.
PCR-RFLP of ribosomal internal transcribed spacers highlights inter and intra-species variation among Leishmania strains native to La Paz, Bolivia
(Elsevier BV, 2010) Rosio Buitrago; Elisa Cupolillo; Brigitte Bastrenta; François Le Pont; E. Martínez; Christian Barnabé; Simone Frédérique Brénière
POLYMERASE CHAIN REACTION DETECTION AND SEROLOGIC FOLLOW-UP AFTER TREATMENT WITH BENZNIDAZOLE IN BOLIVIAN CHILDREN INFECTED WITH A NATURAL MIXTURE OF TRYPANOSOMA CRUZI I AND II
(American Society of Tropical Medicine and Hygiene, 2006) María Flóres-Chávez; Marie-France Bosseno; Brigitte Bastrenta; JOSE-LOUIS ALCAZAR DALENZ; Mireille Hontebeyrie; Susana Revollo; Simone Frédérique Brénière
Thirty-five Bolivian children (5-10 years of age) seropositive for infection with T. cruzi underwent specific chemotherapy with benznidazole. Before treatment, 57.1% had a positive parasitologic diagnosis. Some patients presented an early conversion by polymerase chain reaction of blood samples, while others were still positive four and seven months after the end of the treatment, which indicated an absence of parasite clearance. Strain typing showed that most patients were infected by a mixture of clones I and II of T. cruzi. Serologic conversion in conventional tests and antibodies to shed acute-phase antigen were observed in two and four patients, respectively. For the other patients, the average rate of antibody decay was half the initial rate. The parasitologic and serologic data indicated that chemotherapy acts throughout the course of infection in a long-lasting process in which the decrease of specific antibody production is related to the reduction of the live parasite load.
Polymerase chain reaction-based identification of New World Leishmania species complexes by specific kDNA probes
(Elsevier BV, 1999) Simone Frédérique Brénière; Jenny Telleria; Marie France Bosseno; R Buitrago; Brigitte Bastrenta; G Cuny; Anne‐Laure Bañuls; S. Brewster; Douglas C. Barker
Sylvatic population of Triatoma infestans from the Bolivian Chaco: from field collection to characterization
(Instituto Oswaldo Cruz, Ministério da Saúde, 2000) François Noireau; Brigitte Bastrenta; Silvia Catalá; Jean-Pierre Dujardin; Francisco Panzera; Magdalena Torres; Ruben Pérez; Cléber Galvão; José Jurberg
A sylvatic Triatoma infestans DM (dark morph) population detected in the Bolivian Chaco was characterized and compared with various domestic ones. The degree of differentiation of DM was clearly within the T. infestans intra-specific level. Nevertheless marked chromatic and morphometric differences as well as differences in antennal pattern, chromosome banding and randomly amplified polymorphic DNA support the hypothesis of a distinct population. Continuous exchange of insects between wild and domestic habitats seems unlikely in the Chaco.