Browsing by Autor "Camilo Racedo"

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Genome mining to elucidate antimicrobial and plant growth promoting potentials in Lactic acid bacteria
(Springer Science+Business Media, 2025) Christian Vargas; Lina Marcela Botero Rute; Edwin Rodríguez; Lorena Dávila; Camilo Racedo; Claudia Barrera; Adriana Bernal; Hugo Jiménez; Paola Zuluaga; Carolina González Almario
Abstract The pangenome of lactic acid bacteria (LAB) was constructed with five bacterial strains isolated from oat silage produced by smallholder farmers from a region in the highlands of the Colombian Andes. The goal of this work was to use genome mining to elucidate some potential mechanisms of action associated with plant growth promotion and biocontrol in LAB. Multiple genes were uncovered, including those associated with antibiosis and antimicrobial compound production, genes that induce plant defense and phytohormone activation. Additionally, genome sequencing suggested mechanisms for LAB to protect against abiotic and biotic stress. Furthermore, genes that may improve phosphorus availability and those that might stimulate root growth were present. We then tested some of these findings in vitro . For instance, the biocontrol activity of these strains was evaluated against the fungal pathogens Fusarium oxsyporum f. sp. cubense ( Foc R1 and Foc TR4) and the bacterial pathogen Ralstonia solanacearum ( Rs ). All tested strains showed the ability to significantly inhibit pathogen growth in vitro . The capability of LAB to grow at high concentrations of fusaric acid was demonstrated, suggesting that LAB might have detoxification mechanisms to compete with other microorganisms. Also, the ability of LAB to solubilize phosphate and produce IAA was demonstrated. These two properties might be involved in plant-growth promotion. Here we show that genomic sequencing can be an efficient tool to identify putative biocontrol or plant-growth promoting related sequences for genome mining.
Uso de la biodiversidad para mejorar la agricultura: tendencias en la investigación y el mercado en biofertilizantes en Colombia
(Universidad de Los Andes, 2024) Paola Reyes; J. C. Oliveros; Camilo Racedo; Adriana Bernal Giraldo
The use of biofertilizers has increased in recent decades as a sustainable alternative to chemical agro-inputs. Although the use of agrochemicals is still more common than the use of biofertilizers, there has been an increasing trend towards more sustainable methods, including biofertilizers. Even though Colombia is a country with great biotechnological potential and high biodiversity, it is not a leader in the production of biofertilizers, which limits its capacity to solve food requirements sustainably. Thus, this review seeks to understand the current trends in Colombian biofertilizers, from the science behind their production to the challenges the country faces in the use of microbial biodiversity for agriculture. This process was carried out through an information search in indexed databases, compiling information on biofertilizers registered in Colombia and considering characteristics such as microbial composition, methodologies, market trends, bioproduct composition, formulation, and current regulations. Finally, a prospect is presented regarding the future destiny of biofertilizers in Colombia, their possible implementation, effects on agricultural production, and methods to accelerate their development, seeking to optimize their use and adoption in the country.