Browsing by Autor "Carlier, Y"

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Bioenergetic studies in residents at high altitude (2,850 m) with asymptomatic Chagas'disease
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1985) Carlier, Y
Summary. Cardiovascular response and working capacity were studied at rest and during a 125 W exercise in residents at high altitude (2,850 m), comparing 2l asymptomatic (normal EKG) bul T. cruzi infected subjects (positive serology) to 2l healty controls (negative serology). Ages, anthropometric, nutritional. and hematological data were similar in the two groups (P > 0.05), indicating the homogeneily of the studied population. Cardiac axis, heart rales, and oxygen uptakes, were not different between the two groups, at rest and/or during exercise (P > 0.05). At rest, diastolic and systolic pressures were significantly lower in the infected group than in the control group (P < 0.05). During exercise and recovery at the 1st and 5th min, diastolic pressure was also significantly lower in infected patients than in conlrol group whereas systolic pressure was significantly lower only during recovery at 5th min. The calculated VO, max were similar in the two groups, indicating a normal working capacity for the studied asymptomatic infected patients. The altitude does not seem to affect the responses to exercise, since the results obtained at high altitude were similar to those obtained at sea level (Macedo et al., 1973).
Specific immunodiagnosis of Chagas disease: immunodiffusion test using a specific serum anti-Trypanosoma cruzi component 5.
(1987) Breniere, F S; Carlier, Y; Carrasco, R; Molinedo, S; Lemesre, J L; Desjeux, P; Afchain, D
A micro double diffusion test (MD), allowing the identification of precipitation brand 5 by identity reaction, using a rabbit specific anti-component 5 serum, was evaluated for the immunological diagnosis of Chagas' disease. The previous studies on the Trypanosoma cruzi specificity of component 5[g] were completed, showing it to be absent in Leishmania brazilienis, but present in different strains of T. cruzi. 200 sera from Bolivian patients were studied. (88 with a positive xenodiagnosis, 45 with mucocutaneous leishmaniasis but without Chagas' disease, and 67 controls). Band 5 was found in 74 (84.1%) of the sera with positive xenodiagnosis but was never found either in the leishmaniasis or in the control groups. MD, allowing an easy detection of T. cruzi specific band 5, cheap and simple to perform, can be recommended in association with other serological tests, when highly specific immunodiagnosis of Chagas' disease is required.
The interest of immunoprecipitation tests in the immunological diagnosis of Chagas'disease
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1985) Carlier, Y
Summary. lmmunoelectrophoresis (lEP) and a double diffusion microtest (MD) were evaluated for ¡mmunological diagnosis of Chagas' d¡sease, using 527 sera from Bolivian patients. The specificity of, the tests was given by the identification of precipitating ant¡- bodies anti-component 5, previously demonstrated as specific of Ttypanosoma cruzi. IEP showed 1 to 14 precip¡tation lines in 96 per cent of the sera, both wilh parasitological (positive xenodiagnosis) or serological conf¡rmalion of T. cruzi infection, whereas the control sera were all negative. Prec¡p¡tat¡on line 5, ¡dentified by its parlicular pattern, was present in 70 per cent of the same sera. ln MD, prec¡pitation line 5, identified by ident¡ly reaction with a rabbit anti-component 5 specif¡c serum, was found in 80.2 per cent of all the serological positive sera: 84.1 per cent of lhe sera with posit¡ve and 72.2 per cent of the sera w¡th negative xenod¡agnosis (P 0.05). Line 5 was never found, ne¡ther in the le¡shmaniasis group (com¡ng from an area wilhout Chagas' d¡sease) nor in the control group. ln another part¡cular leishmanias¡s group, coming from an area endemic lor both ¡nfect¡ons,31.7 per cent of the sera were line 5 pos¡tive, indicating assoc¡ated Chagas' disease. Consequently, the ¡mmunoprecipitation test, allow¡ng the detection of. T cruzi spec¡f¡c line 5, ¡s cheap and simple to perform. lt can be recommended ¡n assoc¡ation with the other serolog¡cal tests wh¡ch are more sensit¡ve, when highly specific ¡mmunod¡agnosis of Chagas' disease is requ¡red.