Browsing by Autor "Carlos Areche"

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Comprehensive Phytochemical Profile of Leaves, Stems and Fruits from Orthopterygium huaucui (A. Gray) Hemsl. and their Antioxidant Activities
(Wiley, 2024) Marcos Hernández; Grover Castañeta; Mario J. Simirgiotis; Beatriz Sepúlveda; Carlos Areche
Orthopterygium huaucui, commonly known as "Pate", is a medicinal shrub belonging to the Anacardiaceae family used locally to treat burns and stomach pains. Endemic to Peru, chemical studies on O. huaucui are limited. In this study, Ultra-High Performance Liquid Chromatography Quadrupole/Orbitrap Electrospray Ionization Tandem Mass Spectrometry (UHPLC Q/Orbitrap/ESI/MS/MS) was used to identify secondary metabolites in leaves, stems and fruits, and the antioxidant capacities of the different parts were compared. In addition, several compounds such as methyl gallate, gallic acid, kaempferol, quercetin, and quercetin 3-O-β-glucuronide were successfully isolated from the methanolic extract of the leaves of this species for the first time. Untargeted UHPLC Q/Orbitrap/ESI/MS/MS analysis tentatively identified seventy-six compounds in the different parts of the plant, showing that this species as an interesting source of flavonoids, procyanidins and tannins. The phenolic content in leaves and stems was 334.31±4.34 and 295.18±6.38 gallic acid equivalents/100 g dry plant, respectively, while that of fruits was lower (99.92±5.45 mg/100 g). Leaves had twice the flavonoid content than fruits (210.38±3.85 versus 87.42±3.85 quercetin equivalents/100 g). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) results indicated high antioxidant activity in all parts, with stems and leaves showing IC50 of 12.8 μg/mL, and fruits showing less activity (IC50=38.6 μg/mL). The Oxygen Radical Absorbance Capacity (ORAC) test showed higher antioxidant values in the stems (467.82±21.17 μmol Trolox equivalents/100 g). This study provides valuable information on the chemistry of O. huaucui and highlights its antioxidant potential, especially in leaves and stems.
Dictyostelium discoideum as a Platform to Assess the Cytotoxicity of Marine Algal Extracts: The Case of Glossophora kunthii
(Multidisciplinary Digital Publishing Institute, 2025) Sheyla J. Figueroa-Valencia; Marcos Hernández; Grover Castañeta; Ian Pérez Ramírez; Alejandro Ardiles; Elizabeth Figueroa-Valencia; Teresa Cano de Terrones; Francisco P. Chávez; Carlos Areche
The social amoeba Dictyostelium discoideum is a versatile biological model widely used in drug discovery and studying cellular stress responses. However, its application for cytotoxicity evaluation of natural products, particularly algal-derived compounds, remains underutilized. In this study, we developed a high-throughput developmental assay in D. discoideum to analyze the cytotoxicity of acetone and methanol extracts from the Peruvian seaweed Glossophora kunthii. Our results showed that the acetone extract caused a transient delay in the social development of the amoeba. In contrast, the methanol extract exhibited no significant effects, even at high extract concentrations. UHPLC/Orbitrap/ESI/MS/MS analysis tentatively identified ten major compounds, including pachydictyol A and dictyotriol A diacetate. The presence of diterpenes, such as dictyotadiol and pachydictyol A, previously reported to exhibit moderate cytotoxic activity, likely explains the developmental delay observed with the acetone extract. This study highlights the utility of D. discoideum as a scalable cytotoxicity screening platform within algal pharmacognosy, facilitating the early identification of non-toxic marine natural products suitable for further biomedical and biotechnological development.
Liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry characterization of depsides and depsidones from the Chilean lichen Parmotrema perlatum
(SAGE Publishing, 2024) Grover Castañeta; Beatriz Sepúlveda; Carlos Areche
Lichens are recognized by their unique compounds and diverse applications in food, medicines, and cosmetics. Using ultra-high pressure liquid chromatography, coupled with a high-resolution mass spectrometer, metabolomic profiling of the lichen Parmotrema perlatum, from a methanolic extract, was performed. Based on characteristic fragmentation patterns, twenty-five lichenic substances were tentatively identified including 5 depsides, 12 depsidones, 2 diphenyl ethers, 1 aromatic considered as possible artifact, 1 dibenzofuran, 1 carbohydrate, 1 organic acid, and 2 undefined compounds. To the best of our knowledge, this is a more complete report of their phytochemistry from P perlatum. Our findings of the P perlatum profile may contribute and complement the current data of the Parmotrema genus.
Terpenoids and sterols from lichens
(Springer Science+Business Media, 2024) Grover Castañeta; Boris M. Mamani; Flanklin Mita; Mario J. Simirgiotis; Carlos Areche
Untargeted Metabolomics by Using UHPLC–ESI–MS/MS of an Extract Obtained with Ethyl Lactate Green Solvent from Salvia rosmarinus
(Multidisciplinary Digital Publishing Institute, 2022) Grover Castañeta; Nicolás Cifuentes; Beatriz Sepúlveda; Daniela Bárcenas-Pérez; José Cheel; Carlos Areche
Salvia rosmarinus (Lamiaceae), previously known as Rosmarinus officinalis, is a plant cultivated worldwide, native to the Mediterranean region. Its leaves are traditionally used for cooking. This species possesses numerous biological activities, including antioxidant, antimicrobial, anticancer, anti-inflammatory, and hepatoprotective properties. These biological properties are due to the presence of phenolic compounds, including rosmarinic acid and phenolic diterpenoids, such as carnosic acid and carnosol. In this study, we investigated the chemical composition of a green extract obtained by maceration with ethyl lactate for the first time. Seventy-five compounds were tentatively identified by UHPLC–ESI–MS/MS, including six organic acids, six cinnamic acid derivatives, five fatty acids, eighteen flavonoids, and thirty-eight terpenoids. Thus, abietane-type diterpenoids from the ethyl lactate extract were the predominant diterpenoids in the Chilean S. rosmarinus species, in contrast to the Chinese species, in which labdane and isopimarane-type diterpenoids were found for the first time. Finally, our study confirms that the extraction of S. rosmarinus with green ethyl lactate as a solvent is efficient and sustainable for the identification of flavonoids, phenols, and terpenoids from leaves.