Browsing by Autor "Carmine Lobo"

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    Aislamiento y cultivo de células madre posnatales de dientes primarios
    (LA Referencia, 2014) Daniela Olávez; Siham Salmen; Karla Padrón; Carmine Lobo; Nancy Díaz; Lisbeth Berrueta; Eduvigis Solórzano
    Antecedentes: Las enfermedades degenerativas representan en la actualidad un problema de salud pública, de allí que el desarrollo y aplicación de estrategias que permitan restituir parcial o totalmente tejidos afectados tiene un especial interés en el campo biomédico. Una de las estrategias terapéuticas se basa en el uso de células mesenquimáticas, en particular las provenientes de la pulpa dental. Objetivo: Desarrollar un cultivo de células mesenquimáticas a partir de pulpa dental de dientes deciduos. Métodos: Incluyó el aislamiento de células mesenquimáticas de la pulpa dental de 2 caninos temporarios extraídos con indicaciones terapéuticas, y cultivo en medio D-MEM con SBF al 20% a 37◦C en atmósfera húmeda, realizando cambios de medio de cultivo cada tres días y observación cada 7 días. La confluencia del 80-90% se logró luego de 3 semanas, se realizó la tinción con DAPI y STRO-1, y análisis mediante citometría de flujo y microscopia de fluorescencia. Resultados: Los análisis muestran células adherentes purificadas con morfología fusiforme similar a fibroblastos y aparición de conglomerados semejantes a unidades formadoras de colonias. El total de la población mostró un 17% de positividad al STRO-1, mientras que la población de mayor tamaño y más compleja mostró una positividad del 26%, asimismo STRO-1 se localizó preferencialmente en las unidades formadoras de colonias. Conclusión: Con el protocolo descrito se logró un cultivo de células mesenquimaticas extraídas de pulpa dental en dientes deciduos, como punto de partida para futuros ensayos terapéuticos y su aplicación en la regeneración tisular.
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    Características clínicas y cefalométricas de pacientes respiradores bucales atendidos en el módulo Santa Elena de la Facultad de Odontología Universidad de Los Andes Mérida – Venezuela
    (Universidad Internacional de La Rioja, 2021) Sandra Parra; Kelly Uzcátegui; Carmine Lobo
    Mouth breathing is a common habit in patients of developing age, the tongue is located in a descending position, deforming the palate as it deepens. According to the literature, there are clinical and cephalometric variations in detectable mouth-breathing children, however, no studies have been found related to the description of the clinical and cephalometric characteristics of mouth-ventilators attended at the Children's Clinic of the Santa Elena Module of the Faculty of Dentistry, ULA. The objective was to determine the clinical and cephalometric characteristics obtained from the medical records of mouth-breathing patients treated at the Children's Clinic of the Santa Elena Module between 2008 and 2018. The research was descriptive in scope with a non-experimental cross-sectional design. The population was determined by the medical records of the children seen at the Children's Clinic of the Santa Elena Module diagnosed as oral respirators between 2008 and 2018. Descriptive statistics were used for the presentation of the results, and for the processing of the data by the SPSS software. . This study concluded that the mesoprosopo facial type, convex profile, as well as the deep pointed palate predominated in terms of clinical characteristics. Regarding the cephalometric characteristics, they were found in upper and lower E, ANB, SN-SGN and ENA-Me were above the norm, while the SNB angle and the interincisal angle were lower than the norm.
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    Isolation and Culture of Postnatal Stem Cells from Deciduous Teeth
    (2014) Daniela Olávez; Siham Salmen; Karla Padrón; Carmine Lobo; Nancy Díaz; Lisbeth Berrueta; Eduvigis Solorzanio
    Background: Currently, degenerative diseases represent a public health problem; therefore, the development and implementation of strategies to fully or partially recover of damaged tissues has a special interest in the biomedical field. Therapeutic strategies based on mesenchymal stem cells transplantation from dental pulp have been proposed as an alternative. Purpose: To develop a mesenchymal stem cells culture isolated from dental pulp of deciduous teeth. Methods: The mesenchymal stem cells isolation was performed from dental pulp of two deciduous canines, freshly extracted with therapeutic indication. Specimens were cultured in D-MEM medium with 20% FBS at 37 °C and 5% CO2. The medium was changed every three days. 80-90% confluence was achieved after three weeks. Cells were stained with DAPI and STRO-1 and analyzed through flow cytometry and fluorescence microscopy. Results: The analysis showed that adherent cells had a fusiform fibroblast-like morphology and colony forming units (CFUs) were observed. 17% of the whole population was STRO-1+ and 26% of the larger and more complex population was positive to this antigen. Additionally, STRO-1+ cells were localized preferential in UFCs. Conclusions: The protocol described here could be used to enriching mesenchymal stem cells from dental pulp extracted from deciduous teeth and as a starting point for future therapeutic trials and their application in tissue regeneration.KEYWORDScultured cells; stem cells; deciduous teeth