Browsing by Autor "Carolina Cura"
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Item type: Item , Analytical Performance of a Multiplex Real-Time PCR Assay Using TaqMan Probes for Quantification of Trypanosoma cruzi Satellite DNA in Blood Samples(Public Library of Science, 2013) Tomás Duffy; Carolina Cura; Juan Carlos Ramı́rez; Teresa Abate; Nelly M. Cayo; Rudy Parrado; Zoraida Díaz Bello; Elsa Velázquez; Arturo Muñoz-Calderón; Natalia JuizThe performing parameters of this assay encourage its application to early assessment of T. cruzi infection in cases in which serological methods are not informative, such as recent infections by oral contamination or congenital transmission or after transplantation with organs from seropositive donors, as well as for monitoring Chagas disease patients under etiological treatment.Item type: Item , Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients(Elsevier BV, 2015) Juan Carlos Ramı́rez; Carolina Cura; Otacílio C. Moreira; Eliane Lages-Silva; Natalia Juiz; Elsa Velázquez; Juan David Ramírez; Anahí Alberti; Paula Pavía; María Flóres-ChávezItem type: Item , Interest and limitations of Spliced Leader Intergenic Region sequences for analyzing Trypanosoma cruzi I phylogenetic diversity in the Argentinean Chaco(Elsevier BV, 2010) Nicolás Tomasini; Juan José Lauthier; María M. Monje Rumi; Paula G. Ragone; A. ALBERTI D'AMATO; Cecilia Pérez Brandán; Carolina Cura; Alejandro G. Schijman; Christian Barnabé; Michel TibayrencItem type: Item , International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients(Public Library of Science, 2011) Alejandro G. Schijman; Margarita Bisio; Liliana Orellana; Mariela Sued; Tomás Duffy; Ana María Mejía‐Jaramillo; Carolina Cura; Frederic Auter; Vincent Véron; Yvonne QvarnströmThis study represents a first crucial step towards international validation of PCR procedures for detection of T. cruzi in human blood samples.Item type: Item , Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples(Public Library of Science, 2015) Carolina Cura; Tomás Duffy; Raúl Horacio Lucero; Margarita Bisio; Julie Péneau; Matilde Jiménez‐Coello; Eva Muñoz; María-José Giménez; Edward Valencia Ayala; Sonia A. KjosTyping is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production.