Browsing by Autor "D. Afchain"

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Evaluation of a Competitive Antibody Enzyme Immunoassay for Specific Diagnosis of Chagas' Disease
(American Society of Parasitologists, 1989) Washington R. Cuna; Carlos A. Rodríguez; Faustino Torrico; D. Afchain; Marc Loyens; P. Desjeux
A competitive enzyme immunoassay based on the use of a monoclonal antibody (MAb) specific for "component 5" of Trypanosoma cruzi was evaluated. The antigenicity and immunogenicity of this component has been observed in natural and experimental infections. The studies were conducted in an area of Bolivia where mixed infections with Leishmania braziliensis are frequent and present a problem in the accurate diagnosis of T. cruzi infections. The specificity and sensitivity of this assay as compared to the indirect immunofluorescence and ELISA tests were demonstrated. The present test has proved to be more specific than the immunofluorescence and ELISA tests.
Specific and Sensitive Immunological Diagnosis of Chagas' Disease by Competitive Antibody Enzyme Immunoassay Using a Trypanosoma cruzi-Specific Monoclonal Antibody
(American Society of Tropical Medicine and Hygiene, 1986) Jean-Loup Lemesre; D. Afchain; Oscar Orozco; M. Loyens; Simone Frédérique Brénière; P. Desjeux; Y Carlier; Ubaldo O. Martín; J. A. Nogueira-Queiroz; D. Le Ray
Coexistence of Chagas' disease with leishmaniasis and T. rangeli infection in endemic areas and cross-reactivity between corresponding etiological agents can confuse the immunodiagnosis of Chagas' disease. A discriminative serological test could therefore represent a major advance in specific immunodiagnosis. A competitive antibody enzyme immunoassay against a component 5-enriched preparation, using a T. cruzi species-specific monoclonal antibody has allowed development of a specific serodiagnosis of Chagas' disease with high sensitivity (96.6% in undetermined and chronic phases of infection). This test can differentiate Chagas' disease from other cross-reacting parasitic diseases in areas where concomitant infections are unknown or suspected.
Specific IgE antibodies to Leishmania Braziliensis in patients with mucocutaneous leishmaniasis
(Elsevier BV, 1983) D. Afchain; P. Desjeux; C. La Fuente; D. Le Ray; Jean-Yves Cesbron; J.L. Neyrinck; A Capron
Specific immunodiagnosis of Chagas disease: immunodiffusion test using a specific serum anti-Trypanosoma cruzi component 5.
(National Institutes of Health, 1987) Simone Frédérique Brénière; Yves Carlier; Roxana Carrasco; S Molinedo; Jean-Loup Lemesre; P. Desjeux; D. Afchain
A micro double diffusion test (MD), allowing the identification of precipitation brand 5 by identity reaction, using a rabbit specific anti-component 5 serum, was evaluated for the immunological diagnosis of Chagas' disease. The previous studies on the Trypanosoma cruzi specificity of component 5[g] were completed, showing it to be absent in Leishmania brazilienis, but present in different strains of T. cruzi. 200 sera from Bolivian patients were studied. (88 with a positive xenodiagnosis, 45 with mucocutaneous leishmaniasis but without Chagas' disease, and 67 controls). Band 5 was found in 74 (84.1%) of the sera with positive xenodiagnosis but was never found either in the leishmaniasis or in the control groups. MD, allowing an easy detection of T. cruzi specific band 5, cheap and simple to perform, can be recommended in association with other serological tests, when highly specific immunodiagnosis of Chagas' disease is required.
Trypanosoma cruzi: expression of antigenic component 5 among 35 laboratory clones obtained from 18 different isozymic variants
(University of São Paulo, 1987) Simone Frédérique Brénière; Susana Revollo; T Caillard; Eric Velatte; Dominique Legrand; D. Afchain; P. Desjeux
Two monoclonal antibodies anti-component 5 of Trypanosoma cruzi (I-35/115 and II-190/30) were tested in IFA and ELISA respectively against 35 T. cruzi laboratory clones. Among the 35 clones tested, 18 different isozyme patterns were detected. All clones were recognized by both monoclonal antibodies except one clone which did not react with II-190/30. These results support the universal expression of specific component 5 within the taxon T. cruzi.