Browsing by Autor "Daniels D. Dion"

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Effect of VIUSID-Agro on the conversion of somatic embryos of coffee (Coffea arabica L.) cv. Red Caturra rojo-884
(Academic Journals, 2021) Posada-Pérez Laisyn; Barbón Rodríguez Raúl; Capote Pérez Alina; Pérez Pérez Anabel; Padrón Montesino Yenny; Kukurtcu Bulent; Daniels D. Dion; Reyes Guillermo; G oacute mez Kosky Rafael
A critical factor in the in vitro propagation protocol via somatic embryogenesis of coffee is the survival and growth of plants under ex vitro conditions. The objective of this work was to determine the effect of VIUSID Agro™ during the conversion of somatic embryos of coffee cv. Red Caturra rojo-884 in semi-controlled culture conditions. Two solutions (0.5 and 0.8 mL L-1) of the biostimulant were evaluated on in vitro plant. Applications were done twice daily for the first three days and thereafter once a day from day 7 to 90 days after transplanting. Survival rate at 15 days and morpho-physiological characteristics at 30, 60 and 90 days after transplanting were evaluated. All treatments with VIUSID-Agro™ and the control resulted in 100% survival. The concentration of 0.5 mL L-1 of VIUSID-Agro™ registered the better results in morpho-physiological variables. These results constitute the first report of the use of VIUSID Agro™ on in vitro plant of coffee during acclimatization phase.   Key words: Ex vitro acclimatization, coffee, conversion, somatic embryos, VIUSID Agro™.
Effects of different culture conditions (photoautotrophic, photomixotrophic) and the auxin indole-butyric acid on the in vitro acclimatization of papaya (Carica papaya L. var. Red Maradol) plants using zeolite as support
(Academic Journals, 2015) Laisyn Posada Pérez; Padron Montesinos Yenny; Gonzalez Olmedo Justo; Rodr iacute guez Sanchez Romelio; Norman Montenegro Osvaldo; Barb oacute n Rodriguez Raul; Hurtado Ribalta Ortelio; René C. Rodríguez-Escriba; Daniels D. Dion; G oacute mez Kosky Rafael
Plant regeneration of papaya via organogenesis and somatic embryogenesis has been successful; however, the biggest problem of in vitro culture of this species is the acclimatization of regenerated plants, where over 70% of the plants are lost before being planted in the field. Decreasing the relative humidity inside the culture vessel and thus increasing the ventilation, appears to have a greater effect on the adaptation of papaya plants, strengthening the function of the stomata and with this, allowing better control of water loss from the leaves. The aim of this study was to determine the effects of different concentrations of sucrose and indole-butyric acid (IBA) on rooting and in vitro acclimatization of plants using sterile zeolite as support and culture vessels with increased ventilation. Three concentrations of sucrose (0, 10 and 20 g L-1) were studied with and without auxin and as the control treatment, the rooting culture medium with agar during 17, 27 and 37 culture days. The highest percentage of rooting was recorded at 37 culture days in the treatment without sucrose and IBA with 80.0% and zeolite as support. The best photosynthetic values were achieved when in vitro shoots were grown in culture medium with auxin and different concentrations of sucrose, even though they were also high in the treatment without the presence of IBA and without sucrose at 17 days of culture. The combined effect of the zeolite, auxin (IBA), without sucrose in the culture medium and increased ventilation allowed photoautotrophic culture conditions which had effect of the increasing plant survival under ex vitro acclimatization conditions. Key words: Carica papaya, photosynthesis, roots formation.
Nuevo explante para la embriogénesis somática en Sorghum bicolor (L.) Mohen
(University "Marta Abreu" of Las Villas, 2015) Silvio Martínez Medina; Raúl Collado; Raúl Barbón; Mayelín Rodríguez; Marta Pérez; Luís Rojas; Miguel Á. Tejada; Daniels D. Dion; Berkis Roque; Dámaris Torres
This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-05’ starting from callus obtained from sections of in vitro shoots. For the formation of callus, different concentrations of 2,4-D were studied as well as three concentrations of ascorbic acid to eliminate the phenolic oxidation. To increase the percentage of callus formation with embryogenic structures, different segments of the shoots were used. For the formation of somatic embryos, different concentrations of 2,4-D; 6-BAP and L-Proline were added to the culture media. The greatest callus formation (50%) was obtained in the culture medium with 18.1 µM of 2,4-D. When 50 mg l -1 of ascorbic acid was added to the culture medium, the percentage of callus formation increased to 67.5%, and was couple with absence of oxidation of the medium and the explant. The frequency of callus formation with embryogenic structures increased to 95% with the use of segment 1 of the shoot sections in vitro as explant. The greatest number of somatic embryos per callus was obtained when 2,4-D was reduced to 4.52 µM, combined with 22.2 µM of 6-BAP and 500 mg l -1 of L-Proline (41.88). Histological analysis confirmed that structures formed in callus came from a somatic embryogenesis process. For the first time, the efficient formation of somatic embryos of sorghum section 1 of in vitro shoots in vitro was obtained. Keywords: 2,4-D, shoots in vitro, callus, somatic embryos