Browsing by Autor "Dariel Cabrera Mederos"

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An unwanted association: the threat to papaya crops by a novel potexvirus in northwest Argentina
(2022) Dariel Cabrera Mederos; Humberto Debat; Carolina Torres; Orelvis Portal; Margarita Jaramillo Zapata; Verónica Trucco; Ceferino Rene Flores; Claudio Ortiz; Alejandra Badaracco; Luis Acuña
Abstract An emerging virus isolated from papaya ( Carica papaya ) crops in northwestern (NW) Argentina was sequenced and characterized using next-generation sequencing. The resulting genome is 6,667-nt long and encodes five open reading frames in an arrangement typical of other potexviruses. This virus appears to be a novel member within the genus Potexvirus . Blast analysis of RNA-dependent RNA polymerase (RdRp) and coat protein (CP) genes showed the highest amino acid sequence identity (67% and 71%, respectively) with pitaya virus X. Based on nucleotide sequence similarity and phylogenetic analysis, the name papaya virus X is proposed for this newly characterized potexvirus that was mechanically transmitted to papaya plants causing chlorotic patches and severe mosaic symptoms. RT-PCR based detection of papaya virus X (PapVX) revealed that it is widely present in papaya crops from NW Argentina. The prevalence of PapVX, which seems to be restricted to the NW region of Argentina, and the fact that it has only been detected in this region could be associated with a recent emergence or adaptation of this virus to papaya in NW Argentina.
Isolation and Characterization of Phosphate-Solubilizing Rhizobacteria from Solanum tuberosum with Plant Growth-Promoting Activity
(2026) Michel Leiva-Mora; Pamela Elizabeth Mera Guzmán; Rafael Isaías Mera Andrade; Alicia Monserrath Zabala Haro; Luis Rodrigo Saa; Paúl Loján; Catherine Lizzeth Silva Agurto; Luis Fabián Salazar-Garcés; Betty Beatriz González Osorio; Dariel Cabrera Mederos
Phosphate-solubilizing rhizobacteria associated with the Solanum tuberosum L. cultivar ‘Superchola’ were isolated and characterized to improve our understanding of plant growth promotion in agricultural systems. Bacteria were isolated by serial dilutions, and the morphology of the colonies was characterized on nutrient agar culture medium. In addition, morphological identification was achieved by Gram staining. The ability to solubilize phosphate was assessed in Pikovskaya agar culture medium, while molecular identification involved the amplification of the partial 16S rRNA gene using the polymerase chain reaction. In the Píllaro canton, the highest number of colony-forming units per gram of soil was recorded at 9.72 × 109. Among the isolated strains, 62% exhibited circular morphology, 92% had a smooth texture, and 85% displayed entire margins. Notably, 83% of the isolates were Gram-negative, with 50% exhibiting a bacillary form. The most effective phosphate solubilizers were from the Mocha canton, particularly the isolate CC-FCAGP-BSF6, which showed superior solubilization capacity. Molecular identification revealed bacterial isolates from four genera, i.e., Bacillus, Pseudomonas, Lysinibacillus, and Paenibacillus. These strains exhibited significant phosphate solubilization in vitro and resulted in increased leaf area (0.21–0.49, p = 0.038), fresh mass (0.46–0.87, p = 0.014), dry mass (0.092–0.096, p = 0.047), and leaf area index (0.14–0.33, p = 0.026) in the S. tuberosum cultivar ‘Superchola’ in vitro plants. This study identifies bacterial species associated with the rhizosphere of S. tuberosum in Ecuador and highlights their potential for promoting plant growth and solubilizing phosphates.
Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
(Springer Science+Business Media, 2017) Dariel Cabrera Mederos; Fabián Giolitti; Claudia Nome; Nicolás Bejerman; Orelvis Portal
Papaya ringspot virus–Carica papaya pathosystem
(Elsevier BV, 2020) Dariel Cabrera Mederos; Fabián Giolitti; Michel Leiva Mora; Orelvis Portal
Papaya—I
(Elsevier BV, 2023) Dariel Cabrera Mederos; Fabián Giolitti; Maylín Cruz; Michel Leiva Mora; Verónica Trucco; Orelvis Portal