Browsing by Autor "David E. Lucero"
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Item type: Item , A method for the identification of guinea pig blood meal in the Chagas disease vector, Triatoma infestans(Springer Science+Business Media, 2007) J.C. Pizarro; David E. Lucero; Lori StevensItem type: Item , PCR reveals significantly higher rates of Trypanosoma cruzi infection than microscopy in the Chagas vector, Triatoma infestans: High rates found in Chuquisaca, Bolivia(BioMed Central, 2007) J.C. Pizarro; David E. Lucero; Lori StevensItem type: Item , Sources of Blood Meals of Sylvatic Triatoma guasayana near Zurima, Bolivia, Assayed with qPCR and 12S Cloning(Public Library of Science, 2014) David E. Lucero; Wilma Ribera; J.C. Pizarro; Plaza Carlos; Levi W. Gordon; Reynaldo Peña; L. A. Morrissey; Donna M. Rizzo; Lori StevensWe show that cloning of 12S PCR products, which avoids bias associated with developing primers based on a priori knowledge, detected blood meal sources not previously considered and that species-specific qPCR is more sensitive. All samples identified as positive for a specific blood meal source by the cloning assay were also positive by qPCR. However, not all samples positive by qPCR were positive by cloning. We show the power of combining the cloning assay with the highly sensitive hydrolysis probe-based qPCR assay provides a more complete picture of blood meal sources for insect disease vectors.