Browsing by Autor "Desjeux, P"

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Cutaneous leishmaniasis in Bolivia. A study of 185 human cases from Alto Beni (La Paz Department). Isolation and isoenzyme characterization of 26 strains of Leismania brasiliensis brasiliensis
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1987) Desjeux, P
Abstract. A clinical, serological, parasitological and therapeutic study of cutaneous leishmaniasis was carried out in a low sub-andean area (250-800 metres) of the La Paz Department, Bolivia. A team of seismic prospectors (350 workers) was surveyed for 12 months. Of 200 suspected cases of cutaneous leishmaniasis, 185 were serologically or parasitologically confumed (incidence 52'8V"). Those exposed to the greatest risk of infection were working in a virgin forest environment. Leishmanial organisms were isolated from 26 of the workers, either by in aito cultivation or inoculation into hamsters. Isoenzyme characterization of the organisms by cellulose acetate electrophoresis showed üem to be Leisbrunia brasili¿nsis brasiliensis. The results of treatrnent of 168 patients with a pentavalent antimonial drug are also reported.
Generic sodium stibogluconate is as safe and effective as branded meglumine antimoniate, for the treatment of tegumentary leishmaniasis in Isiboro Secure Park, Bolivia.
(2006) Bermúdez, H; Rojas, E; Garcia, L; Desjeux, P; Dujardin, J-C; Boelaert, M; Chappuis, F
Human cutaneous leishmaniasis (CL) and mucous leishmaniasis (ML) are highly endemic in Isiboro Secure Park, which lies in the Bolivian department of Cochabamba--an area where branded meglumine antimoniate (Glucantime) is expensive and poorly distributed. The safety and efficacy of generic sodium stibogluconate (SSG), from Albert David Ltd, was therefore explored, in CL and ML cases from the park, who were treated with 20 mg/kg.day for 20 and 30 days, respectively. A questionnaire recording adverse effects was completed by a physician in each treatment centre. Efficacy of treatment was assessed at the end of treatment and at follow-ups 1 month and 3, 6 and 12 months later. Overall, 146 patients completed treatment with SSG in 2003-2004. No fatalities or severe adverse effects were reported but mild to moderate adverse effects were noted in 41 (28%) of the patients. The incidence of adverse effects was significantly higher among the cases of ML than among the cases of CL. Of the 86 patients with CL who completed 6 months of follow-up, 81 (94.2%) were considered to have been clinically cured; a comparable cohort of 69 CL cases who had been treated with Glucantime in 2001-2002 showed a similar frequency of clinical cure (90%). Generic SSG was shown to be safe and efficacious for the treatment of tegumentary leishmaniasis in Bolivia. Being several times cheaper than Glucantime, it could contribute to improving the access of CL and ML patients to treatment, not only in Bolivia but also in other countries of Latin America.
Human visceral leishmaniasis in Bolivia : first proven autochthonous case from "Los Yungas"
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1983) Desjeux, P
On 25th February, 1982, a boy aged two years and two months was brought to the Children's Hospital in La Paz with a six-month history of diarrhoea, vomiting, fever and asthenia. On 14th April, a tentative diagnosis of kala-azar, based on clinical and biological features, was made. A sample of bone marrow showed a few extracellular amastigote forms, confirming the kala-azar diagnosis. The following day, material from a second bonemarrow puncture was sown in NNN medium made with Difco Blood Agar Base (Code B45). After four days, motile promastigote forms were abundant in the culture supernatant. Isoenzyme studies, now in progress, will complete the characterization. All known characters are consistent with Leishmania donovani chagasi.
Identification and purification of a 72 kDa antigen of Leishmania braziliensis braziliensis present on the surface and in the cytoplasm of the promastigotes and its specific recognition by sera from mucocutaneous leishmaniasis patients.
(1988) Kutner, S; Pellerin, P; Brenière, S F; Desjeux, P; Dedet, J P
Las leishmania de Bolivia : II Leismania chagasi s.l., primeros aislamientos en los "Yungas" del departamento de La Paz, comparación isoenzimática de las cepas aisladas de un caso humano autóctono, de perros y del flebótomo Lutzomyia longipalpis
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1983) Desjeux, P
Resumen. Un foco de leishmaniasis visceral ha sido descubierto en los Yungas del Departamento de La Paz. El primer caso humano autóctono ha podido detectarse en 1982 en dicha zona. La infección cutánea y visceral del perro ha sido demostrada. Y finalmente, en este foco, Lutzomyia longipalpis ha sido encontrado espontáneamente infectado (0.8 a 4.2% de los especímenes disectados, según lugar de captura). La tipificación isoenzimática en acetato de celulosa de las cepas procedente del caso humano, de perros (3) y de L. longipalpis (5), comparativamente con cepas de referencia del Brasil y de las costas mediterráneas, ha permitido ligarles al complejo L. donovani s.l. Su similitud, que fue apreciada en 13 enzimas, llevo a afirmar el papel de reservorio doméstico del perro, así como el papel de vector de L. longipalpis en este foco de leishmaniasis visceral. Se discutern los orígenes evaolutivos geográficos de las cepas del complejo l. donovani s.l., y particularmente las de Bolivia.
Las leishmania de Bolivia I : Leishmania braziliensis en los departamentos de La Paz y Beni. Primeros aislamientos de cepas humanas y su caracterización enzimática
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1983) Desjeux, P
Resumen. Los autores efectuaron el estudio clínico, serológico y parasitológico en pacientes procedentes de diferentes zonas donde existe leishmaniasis cutánea y cutáneo-mucosa humana de los Departamentos de La Paz y del Beni. Quince cepas fueron aisladas; la tipificación isoenzimática de diez de ellas (13 enzimas) mediante electroforesis en acetato de celulosa, permitió identificarlas como Leishmania braziliensis braziliensis. Diez enzimas eran discriminadas para el diagnóstico de sub-especie dentro del complejo L. braziliensis. La diversidad del origen geográfico de las cepas, en contraste con su homogeneidad isoenzimática, es discutida.
Les Leishmania de Bolivie. I. Leishmania braziliensis Vianna, 1911 dans les départements de La Paz et du Béni : premiers isolements de souches d'origine humaine. Caractérisation enzymatique
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1986) Desjeux, P
RÉSUMÉ. Les auteurs ont réalisé l'étude clinique, sérologique et parasitologique de patients provenant de différents foyers de leishmaniose cutanée et cutanéo-muqueuse humaine des départements de La Paz et du Béni. Quinze stocks ont été isolés; le typage isoenzymatique de dix d'entre eux (13 enzymes) par électrophorése en acétate de cellulose a permis de les rattacher á, Leishtnania braziliensis. Dix enzymes se sont avérées discriminatives pour le diagnostic d'espéce au sein du complexe L.braziliensis. La diversité de I'origine géographique des stocks, contrastant avec leur homogénéité isoenzymatique, est discutée.
Les Leishmania de Bolivie. II. Leishmania chagasi Cunha et Chagas, 1937. Premiers isolements dans les "Yungas" du Département de La Paz. Comparaison isoenzymatique de souches de l'Homme, du Chien et du Phlébotome Lutzomyia longipalpis (Luts et Neiva, 1912)
(Facultad de Medicina, Enfermería, Nutrición y Tecnología Médica, 1986) Desjeux, P
RÉSUMÉ. Un foyer de leishmaniose viscérale a été mis en évidence dans les Yungas du Département de La Paz. Le premier cas humain autochtone a pu étre diagnostiqué en 1982 dans cette région. L'infection cutanée et viscérale du Chien a été démontrée. Enfin, dans ce méme foyer, Lutzomyia longipalpis a été trouvé spontanément infecté (0,8 á 4,2 % des spécimens disséqués, selon le lieu de capture). Le typage isoenzymatique en acétate de cellulose des stocks provenant du cas humain, de Chiens (3) et de spécimens de Lu. longipalpis (5), comparativement á des souches de référence du Brésil et du pourtour méditerranéen, a permis de les rattacher á Leishmania chagasi. Leur similitude, appréciée sur 13 enzymes, a permis de confirmer le role du réservoir domestique du Chien, ainsi que le róle vecteur de Lu. longipalpis dans ce méme foyer viscéral. Les origines évolutive et géographique des souches du complexe L. donovani s.l., et plus particuliérement celles de Bolivie, sont discutées.
Specific immunodiagnosis of Chagas disease: immunodiffusion test using a specific serum anti-Trypanosoma cruzi component 5.
(1987) Breniere, F S; Carlier, Y; Carrasco, R; Molinedo, S; Lemesre, J L; Desjeux, P; Afchain, D
A micro double diffusion test (MD), allowing the identification of precipitation brand 5 by identity reaction, using a rabbit specific anti-component 5 serum, was evaluated for the immunological diagnosis of Chagas' disease. The previous studies on the Trypanosoma cruzi specificity of component 5[g] were completed, showing it to be absent in Leishmania brazilienis, but present in different strains of T. cruzi. 200 sera from Bolivian patients were studied. (88 with a positive xenodiagnosis, 45 with mucocutaneous leishmaniasis but without Chagas' disease, and 67 controls). Band 5 was found in 74 (84.1%) of the sera with positive xenodiagnosis but was never found either in the leishmaniasis or in the control groups. MD, allowing an easy detection of T. cruzi specific band 5, cheap and simple to perform, can be recommended in association with other serological tests, when highly specific immunodiagnosis of Chagas' disease is required.
Zymodemes of Trypanosoma cruzi isolated from wild mammals in Bolivia.
(1988) Valette, E; Breniere, S F; Le Pont, F; Desjeux, P