Browsing by Autor "E. Ruiz"

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Activité antipaludique du n-hentriacontanol isolé deCuatresiasp (Solanaceae)
(EDP Sciences, 1992) Eric Deharo; Michel Sauvain; Costanzo Moretti; Bernard Richard; E. Ruiz; Georges Massiot
L’activité antipaludique de l’alcool gras n-hentriacontanol isolé d’une Solanacée bolivienne Cuatresia sp. est appréciée in vivo, par le test classique du traitement de 4 jours, sur Plasmodium berghei et P. vinckei chez la souris. Le produit réduit nettement la virulence de l’infection à P. vinckei. Le n-hentriacontanol appartient à une nouvelle classe de composés naturels antimalariques exploitables en thérapeutique.
Antimalarial Activity of Alkaloids from Pogonopus tubulosus
(Wiley, 1996) Michel Sauvain; Christian Moretti; José-Antonio Bravo; Jorgia Callapa; Victoria Muñoz; E. Ruiz; Bernard Richard; Louisette Le Men‐Olivier
The antimalarial activity of the Bolivian medicinal plant Pogonopus tubulosus (D.C.) Schumann was evaluated by in vitro testing on trophozoite stages of resistant and sensitive strains of Plasmodium falciparum and by in vivo tests on P. berghei and P. vinckei petteri in mice. The bark of this medicinal plant yielded three alkaloids: tubulosine, psychotrine, cephaeline. Tubulosine showed an interesting activity in vitro with an IC50 of 0.006 μg/mL against the sensitive strain of P. falciparum and an IC50 of 0.011 μg/mL against the resistant strain of P. falciparum. This compound had good in vivo antimalarial activity with an ED50 of 0.05 mg/kg/day on P. vinckei petteri strain and an ED50 of 0.45 mg/kg/day on P. berghei.
Antimalarial Activity of Alkaloids fromPogonopus tubulosus
(Wiley, 1996) Michel Sauvain; Christian Moretti; José-Antonio Bravo; Jorgia Callapa; Victoria Muñoz; E. Ruiz; Bernard Richard; Louisette Le Men‐Olivier
The antimalarial activity of the Bolivian medicinal plant Pogonopus tubulosus (D.C.) Schumann was evaluated by in vitro testing on trophozoite stages of resistant and sensitive strains of Plasmodium falciparum and by in vivo tests on P. berghei and P. vinckei petteri in mice. The bark of this medicinal plant yielded three alkaloids: tubulosine, psychotrine, cephaeline. Tubulosine showed an interesting activity in vitro with an IC50 of 0.006 μg/mL against the sensitive strain of P. falciparum and an IC50 of 0.011 μg/mL against the resistant strain of P. falciparum. This compound had good in vivo antimalarial activity with an ED50 of 0.05 mg/kg/day on P. vinckei petteri strain and an ED50 of 0.45 mg/kg/day on P. berghei.
Antiprotozoal activity of Jatrogrossidione from Jatropha grossidentata and Jatrophone from Jatropha isabellii 
(Wiley, 1996) Guillermo Schmeda‐Hirschmann; Iván Razmilic; Michel Sauvain; Christian Moretti; Verónica Francisca Loewe Muñoz; E. Ruiz; Elfride Balanza; Alain Fournet
The activity of jatrogrossidione, the main diterpene of Jatropha grossidentata and jatrophone from Jatropha isabellii was determined against Leishmania and Trypanosoma cruzi strains in vitro as well as against Leishmania amazonensis in vivo. Jatrogrossidione showed a strong in vitro leishmanicidal and trypanocidal activity with IC100 of 0.75 and 1.5–5.0 μg/mL, respectively. Under similar conditions, the IC100 of glucantime, ketoconazole and pentamidine towards Leishmania strains were >100, 50–100 and 1 μg/mL, respectively. The IC50 of jatrogrossidione was <0.25 μg/mL against amastigote forms of Leishmania infecting macrophages, with toxicity at concentrations higher than 0.5 μg/mL. BALB/c mice infected with L. amazonensis strain PH 8 were treated 24 h after infection with jatrogrossidione and jatrophone for 13 consecutive days. Jatrophone at 25 mg/kg/day subcutaneously administered was significantly active (p<0.05) against the virulent strain PH 8 of L. amazonesis; it was more active than Glucantime at 112 mg Sbv per kg/day. Subcutaneous administration of jatrophone, however, proved to be too toxic under our assay conditions. Assays of single local treatment on the footpad infection 2 weeks after inoculation of L. amazonensis indicated that jatrogrossidione and jatrophone were inactive at the selected doses.
Antiprotozoal activity of Jatrogrossidione from Jatropha grossidentata and Jatrophone from Jatropha isabellii
(Wiley, 1996) Guillermo Schmeda‐Hirschmann; Iván Razmilic; Michel Sauvain; Christian Moretti; Verónica Francisca Loewe Muñoz; E. Ruiz; Elfride Balanza; A. Fournet
The activity of jatrogrossidione, the main diterpene of Jatropha grossidentata and jatrophone from Jatropha isabellii was determined against Leishmania and Trypanosoma cruzi strains in vitro as well as against Leishmania amazonensis in vivo. Jatrogrossidione showed a strong in vitro leishmanicidal and trypanocidal activity with IC100 of 0.75 and 1.5–5.0 μg/mL, respectively. Under similar conditions, the IC100 of glucantime, ketoconazole and pentamidine towards Leishmania strains were >100, 50–100 and 1 μg/mL, respectively. The IC50 of jatrogrossidione was <0.25 μg/mL against amastigote forms of Leishmania infecting macrophages, with toxicity at concentrations higher than 0.5 μg/mL. BALB/c mice infected with L. amazonensis strain PH 8 were treated 24 h after infection with jatrogrossidione and jatrophone for 13 consecutive days. Jatrophone at 25 mg/kg/day subcutaneously administered was significantly active (p<0.05) against the virulent strain PH 8 of L. amazonesis; it was more active than Glucantime at 112 mg Sbv per kg/day. Subcutaneous administration of jatrophone, however, proved to be too toxic under our assay conditions. Assays of single local treatment on the footpad infection 2 weeks after inoculation of L. amazonensis indicated that jatrogrossidione and jatrophone were inactive at the selected doses.
Liquid chromatographic analysis of cocaine and benzoylecgonine in plasma of traditional coca chewers from Bolivia during exercise
(Elsevier BV, 1997) C Rerat; Michel Sauvain; Pok Phak Rop; E. Ruiz; M. Bresson; Alain Viala