Browsing by Autor "Edilberto R. Rubiolo"

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    Metabolic Post-feeding Changes in Fat Body and Hemolymph of Dipetalogaster maximus (Hemiptera:Reduviidae)
    (Instituto Oswaldo Cruz, Ministério da Saúde, 1998) Lilián E. Canavoso; Edilberto R. Rubiolo
    Lipids and glycogen in fat body as well as the modifications in the wet weight of this organ were evaluated in an unfed insect, Dipetalogaster maximus, on day 5 after adult ecdysis (time 0) and during a 30-day period after ingestion of blood meal, total lipids, high density lipophorin (HDLp), carbohydrates, total proteins and uric acid were determined in the hemolymph during the same period. Fat body wet weight was maximum on day 10 post-feeding and represented on day only 42% of the maximum weight. Lipids stored in the fat body increased up to day 15 reach 24% of the total weight of tissue. Glycogen was maximum on day 20, representing approximately 3% of the fat body weight. HDLp represented at all times between 17-24% of the total proteins, whose levels ranged between 35 and 47 mg/ml uric acid showed at 20, 25 and 30 days similar levels and significantly higher than the one shown at days 10 and 15. Hemolymphatic lipids fluctuated during starvation between 3-4 mg/ml and carbohydrates showed a maximum on day 15 after a blood meal, decreasing up to 0.26 mg/ml on day 25. The above results suggest that during physiological events such as starvation, the availability of nutrients is affected, involving principally the fat body reserves.