Browsing by Autor "Elbert Oberto Reyes Graterol"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item type: Item ,
    CARACTERIZACIÓN DE CÉLULAS CEPILLO DEL CEREBELO TRATADAS POR EXPOSICION PROLONGADA DE LA MUESTRA AL ALCOHOL ISOPROPILICO EN VARIANTE DE IMPREGNACIÓN ARGENTICA
    (2014) Elbert Oberto Reyes Graterol; Sogeilys Milagro García Oduber; Edgard José Laguna Campos; Mario Javier Salas Méndez
    Resumen Las celulas cepillo son neuronas glutamatergicas de la capa granular en la corteza cerebelosa que constan de 3 (tres) o mas subtipos, caracterizados por fenotipos quimicamente distintos, propiedades intrinsecas para formar sinapsis y patrones de descarga particulares. Dichas celulas no fueron identificadas por metodos de impregnacion argentica hasta 1994, cuando Mugnaini realiza su descripcion. En este trabajo presentamos una variante de impregnacion argentica, en el cual las muestras son sumergidas en alcohol isopropilico y luego sometidas a lavados con alcohol etilico proporcionando un proceso de rapida impregnacion que reduce la observacion de precipitados, y donde la cristalizacion se realiza de manera uniforme, lo que permite observar la integridad del cuerpo celular y sus procesos, aumentando tanto la fiabilidad de la tecnica como reproducibilidad de la tincion y facilita la toma de sus dimensiones. Tambien se destaca que el medio de montaje influye en la conservacion de la calidad temporal del preparado, con una mejora de la preservacion utilizando Permount vs Balsamo de Canada. Palabras clave: Celulas cepillo, tecnica de Golgi, alcohol isopropilico, alcohol etilico, balsamo de Canada, Permount. Summary Brush cells in the cerebellum are glutamatergic neurons of the granular layer. They are divided into three or more subtypes and were not identified by silver impregnation methods until 1994, when Mugnaini identified them using a variant of Golgi method. To make evident brush cells of the Wistar rat cerebellum, we developed a modification of the silver impregnation techniques. The improvement of method is due to the immersion of tissue sections in isopropyl alcohol following sequential washes with ethyl alcohol.  It provide a quick impregnation, diminish precipitate outside cells, and the crystallization is more uniform. Morphological and morphometric studies of brush neurons stained with this technique are easy because the cell body and its processes are well observed. We also observed quality differences in the final preparation according with the mounting medium used.  An improvement of the preservation was observed using Permount medium. Keywords : Brush cells, Golgi technique, isopropyl alcohol, ethyl alcohol, Canada balsam, Permount
  • Thumbnail Image
    Item type: Item ,
    Quiste epidermoide intradiploico parietal izquierdo. A propósito de un caso y revisión de la literatura
    (Universidad de Los Andes, 2018) Daniel Alberto Zambrano Lizcano; Elbert Oberto Reyes Graterol; Juan Pablo Chacín González; Frances Stock; Pierina Petrosino Tepedino; Daniel Andrés Ramos Rubio
    Epidermoid cysts are congenital benign, slow growing tumors, that account for 1% of all intracraneal tumors. In 25% of the cases they have an intradiploic localization an are exceptionally infratentorial. We present a 7-year-old escolar patient who presented a growing bulging of the scalp, associated with insidious, holocraneal, low intensity headache. The patient had personal history of migraine and epilepsy with complex partial seizures. Imaging studies reported a well-defined ovoid mass in the left parietal bone with lytic characteristics and 8,8x17mm of diameter. The surgical procedure was successful and the removal of the whole tumor was accomplished. Histopathological studies reported an epidermoid cyst.