Browsing by Autor "Elia M. Chavez-Mamani"

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    A simple parasitological technique to increase detection of Strongyloides stercoralis in Bolivian primary health care system
    (Research Square (United States), 2022) Ivana Camacho-Alvarez; Elia M. Chavez-Mamani; Philippe Goyens; Jenny Marcela Luizaga-López; Mary Cruz Torrico; Laurent Gétaz; Frédérique Jacobs
    Abstract Background: Strongyloides stercoralis is widespread; however, there is limited information on its prevalence owing to laboratory underestimation and low clinical manifestations. The Baermann method and agar culture stand out among the parasitological techniques. Strongyloides stercoralis is present in Bolivia, but its prevalence in children remains unknown. The objective of this study was to estimate the applicability of simple parasitological techniques to increase the detection of this parasite in children living in the tropics. Methods: This cross-sectional study was conducted in a tropical village in Cochabamba, Bolivia. Participants were 304 children aged 5 – 12 years who provided stool samples for different parasitological analyses (direct examination, Ritchie, Barman, and Dancescu techniques), and their parents provided informed consent. Results: Up to 64.8% of pathogenic parasites were detected using the modified Ritchie method. The Baermann technique identified 17.8% of Strongyloides stercoralis cases, and a high sensitivity with respect to the Baermann technique was only for the Dancescu technique (75.9%) that is also specific for Strongyloides stercoralis , followed by 66.7% for the modified Ritchie technique, which is used in second-line care. Discussion: The Baermann technique is the best parasitological option for improving Strongyloides stercoralis diagnosis in the first-line care of the Primary Health Care System. A particular cycle of reinfection, combined with the environment and some other risk factors are related with persistence. Control is difficult without a proper diagnosis, and the Baermann technique is an approach to the solution. We conclude that with a high suspicion of the presence of Strongyloides stercoralis , the use of the Baermann technique is strongly recommended as support for direct examination in primary health care systems especially in tropical areas.
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    A simple parasitological technique to increase detection of Strongyloides stercoralis in Bolivian primary health care system
    (BioMed Central, 2022) Ivana Camacho-Alvarez; Elia M. Chavez-Mamani; Goyens Philippe; Jenny M. Luizaga-López; Mary Cruz Torrico; Laurent Gétaz; Frédérique Jacobs
    The Baermann technique is the best parasitological option for improving Strongyloides stercoralis diagnosis in the first-line care of the Primary Health Care System. A particular cycle of reinfection, combined with the environment and some other risk factors are related with persistence. Control is difficult without a proper diagnosis, and the Baermann technique is an approach to the solution. We conclude that with a high suspicion of the presence of Strongyloides stercoralis, the use of the Baermann technique is strongly recommended as support for direct examination in primary health care systems especially in tropical areas.
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    Additional file 1 of A simple parasitological technique to increase detection of Strongyloides stercoralis in Bolivian primary health care system
    (Figshare (United Kingdom), 2022) Ivana Camacho-Alvarez; Elia M. Chavez-Mamani; Goyens Philippe; Jenny M. Luizaga-López; Mary Cruz Torrico; Laurent Gétaz; Frédérique Jacobs
    Additional file 1. DATASET CBBA_SS: file containing the digitised database of the questionnaires and laboratory records coded.