Browsing by Autor "Elisa Cupolillo"

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A first case of cutaneous leishmaniasis due to Leishmania (Viannia) lainsoni in Bolivia
(Oxford University Press, 2001) E. Martínez; François Le Pont; S. Mollinedo; Elisa Cupolillo
We present the first known case of cutaneous leishmaniasis due to Leishmania (Viannia) lainsoni detected in Bolivia. The parasite was isolated from a young girl living in the subtropical region of Carrasco (900-1000 m above sea level, Caranavi Province, Department of La Paz, Bolivia). The parasite identification was confirmed by multilocus enzyme electrophoresis.
Molecular markers for species identification in the Leishmania subgenus Viannia
(Oxford University Press, 2002) Alexis Mendoza-León; Luís Luís; Octávio Fernandes; Elisa Cupolillo; Lineth García
We have previously identified a novel genomic sequence of 500 bp, the beta 500-DNA sequence, in the subgenus Leishmania (Viannia). This sequence was localized upstream of the beta-tubulin gene. Restriction fragment length polymorphism and hybridization analysis has shown that the beta 500-DNA sequence is specific to this subgenus. A polymerase chain reaction (PCR) assay confirmed this specificity. The beta 500-DNA sequence was apparently absent from the genomic deoxyribonucleic acid of L. colombiensis and L. equatoriensis. These results indicate that a PCR assay based on the beta 500-DNA sequence is likely to be of use to detect and identify Leishmania parasites of this subgenus in clinical samples with high sensitivity, specificity and reliability. The beta 500-DNA sequence can be considered a molecular marker for the subgenus Viannia.
PCR-RFLP of ribosomal internal transcribed spacers highlights inter and intra-species variation among Leishmania strains native to La Paz, Bolivia
(Elsevier BV, 2010) Rosio Buitrago; Elisa Cupolillo; Brigitte Bastrenta; François Le Pont; E. Martínez; Christian Barnabé; Simone Frédérique Brénière
Vectors
(University of São Paulo, 2003) Tello López; Triatoma Williami; Gabriela de; Rosa da; Patrícia Cuervo; Elisa Cupolillo; Thiago Moreno L. Souza; Oliveira; Jankevicius; Gaziri
Chagas disease is the most important parasitic disease in Latin America, as in Mexico, where 1.5-2% of blood donations are contaminated with anti-Trypanosoma cruzi antibody. Through environmental and population based stratification, we estimate that 91 million inhabitants are at risk (78% through residence), 1,768,376 individuals are infected, and mortality may oscillate between 25,500 and 63,000 individuals/yr (830 of these are under 5 yrs old). The disease incidence is estimated at 69,000 cases/yr and approximately 530,500 individuals are currently in chronic phase. More than 96% of the transmission occurs via the vector, and niche modeling with GARP estimates that 67% of the transmission occurs via one of the 6 primary phyllosoma complex species.