Browsing by Autor "Enrique Terrazas Siles"

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    DETERIORO CAUSADO POR MICROORGANISMOS EN TEXTIL ARQUEOLÓGICO Y LIENZOS
    (2012) Nelson Estrada; Guido M. Valverde Garnica; María Teresa Álvarez Aliaga; Enrique Terrazas Siles; Alberto Giménez Turba
    In this paper we identified the organisms that cause deterioration of paintings and a textile fragment from the colonial era, provided by the Ministry of Cul...
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    β-glucoside production by thermophilic bacteria indigenous the bolivian altiplano culture
    (National University of Colombia, 2011) Esther Casablanca Alarcón; Neida Ríos Manríquez; Enrique Terrazas Siles; Ma. Teresa Álvarez Aliaga
    The β-glucosidases possess hydrolytic and transferase activity or transglucosidase. They have various applications; such as biosynthesis of oligosaccharides, production of ethanol using agricultural residues and wine industry. However for industrial application, stability to high temperatures is needed. Therefore a great interesting in the thermophile microorganism study exist. The purpose of this research is to optimize the culture medium of thermophilic anaerobic bacteria to increase the production of β-glucosidase. This enzyme is produced by three isolate bacterial FT3, 2B and P5 which were isolated from the Andean region of Bolivia. FT3 isolate showed β-glucosidase activity of 0.35 (IU/mL). In regards to the optimization of culture medium variables such as nitrogen source, carbon source and pH were taken into account and also the combina- tion with free and encapsulated bacterial cells. Yeast extract was the selected source of nitrogen obtaining an activity of 0.52 (IU/ mL). The optimal pH was 5 obtaining an activity of 0.81 (IU/mL). The selected carbon source was the hydroly- zed wheat straw and quinoa straw obtaining activities of 1.27 and 1.34 (IU/mL), respectively. The cellular localization of β-glucosidase enzyme is extracellular and provides stability to temperature of 80 °C and stability at pH 7.