Browsing by Autor "François Le Pont"

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A first case of cutaneous leishmaniasis due to Leishmania (Viannia) lainsoni in Bolivia
(Oxford University Press, 2001) E. Martínez; François Le Pont; S. Mollinedo; Elisa Cupolillo
We present the first known case of cutaneous leishmaniasis due to Leishmania (Viannia) lainsoni detected in Bolivia. The parasite was isolated from a young girl living in the subtropical region of Carrasco (900-1000 m above sea level, Caranavi Province, Department of La Paz, Bolivia). The parasite identification was confirmed by multilocus enzyme electrophoresis.
A new focus of cutaneous leishmaniasis due to Leishmania amazonensis in a Sub Andean region of Bolivia
(Elsevier BV, 1998) Eliana C. Martinez; François Le Pont; M Torrez; Jenny Telleria; Fernando Regla Vargas; M Muñoz; Simonne De Doncker; Jean‐Claude Dujardin; Jean‐Pierre Dujardin
Additional data on Trypanosoma cruzi isozymic strains encountered in Bolivian domestic transmission cycles
(Oxford University Press, 1986) Michel Tibayrenc; Ary A. Hoffmann; Olivier Poch; Lourdes Echalar; François Le Pont; Jean-Loup Lemesre; P. Desjeux; Francisco J. Ayala
We have collected in Bolivia 212 stocks of Trypanosoma cruzi from domestic transmission cycles and have assayed for nine enzyme systems (11 gene loci). Only a few different isozyme profiles exist, without recombination between them, a situation also encountered in previous Bolivian samples. The 212 stocks, combined with 207 stocks previously studied, have been analysed to uncover any spatial patterns. The frequency of heterozygous strains (2 and 2a) decreases westwards and with increasing altitude. Given that longitude and altitude are correlated with each other, it is not possible to decide which of these two geographic variables is the relevant one, or if both are. These associations might be due to climatic factors. Studies by other authors have shown, however, that heterozygous strains are rare or absent in the Amazon Basin, which is at low altitude.
Co-infection by Leishmania amazonensis and L. infantum/L. chagasi in a case of diffuse cutaneous leishmaniasis in Bolivia
(Oxford University Press, 2002) E. Martínez; S. Mollinedo; M Torrez; M Muñoz; Anne‐Laure Bañuls; François Le Pont
We present the first report of a co-infection by Leishmania amazonensis and L. infantum/L. chagasi isolated in 1993 from a patient with diffuse cutaneous leishmaniasis (DCL), living in the sub-Andean region of Bolivia. This is the third reported case of DCL in Bolivia, but the first one with isoenzymatic identification of the aetiological agents involved and the first one giving evidence for a mixed infection by 2 Leishmania parasites in the same lesion.
Cutaneous leishmaniasis in Bolivia. A study of 185 human cases from Alto Beni (La Paz Department). Isolation and isoenzyme characterization of 26 strains of Leishmania brasiliensis brasiliensis
(Oxford University Press, 1987) P. Desjeux; S. Mollinedo; François Le Pont; Andres Paredes; G Ugarte
A clinical, serological, parasitological and therapeutic study of cutaneous leishmaniasis was carried out in a low sub-andean area (250-800 metres) of the La Paz Department, Bolivia. A team of seismic prospectors (350 workers) was surveyed for 12 months. Of 200 suspected cases of cutaneous leishmaniasis, 185 were serologically or parasitologically confirmed (incidence 52.8%). Those exposed to the greatest risk of infection were working in a virgin forest environment. Leishmanial organisms were isolated from 26 of the workers, either by in vitro cultivation or inoculation into hamsters. Isoenzyme characterization of the organisms by cellulose acetate electrophoresis showed them to be Leishmania braziliensis braziliensis [corrected]. The results of treatment of 168 patients with a pentavalent antimonial drug are also reported.
Description de deux nouvelles espèces de phlébotomes du sous-genre<i>Helcocyrtomyia</i>, du piémont andin bolivien (Diptera, Psychodidae)
(EDP Sciences, 1995) J.M. Torres-Espejo; Gutiérrez Cáceres; François Le Pont
Les auteurs décrivent deux nouvelles espèces de phlébotomes de région subandine de Bolivie, (alt. : 400-2000 m). Elles appartiennent au sous-genre Helcocyrtomyia, dont font partie les vecteurs d'"uta" au Pérou. Lutzomyia guderiani n. sp., qui appartient au groupe sanguinaria, est une espèce anthropophile de forêt primaire et de caféières dans les Yungas. L. rispaili n. sp., décrite du seul sexe mâle, est apparentée au groupe peruensis ; elle n'a été récoltée qu'en forêt primaire subandine de l'Alto Beni, au-dessous de 1000 m.
Descriptions de deux nouvelles espèces de Phlébotomes (Diptera : Psychodidae) de région subandine de Bolivie
(Société entomologique de France, 1991) François Le Pont; P. Desjeux
Résumé. — Description de deux nouvelles espèces de Phlébotomes de région subandine de Bolivie (ait. : 900-2700 m). Lutzomyia torresi n. sp. est très proche de Lutzomyia boliviana qui n'a jusqu'à maintenant été rattachée à aucun groupe ou série ; elle est cavernicole ou péridomestique et se nourrit sur chauves-souris et poulets. Lutzomyia mollinedoi n. sp. est une espèce proche de Lutzomyia oliveirae , Lutzomyia brisolai et Lutzomyia waltoni ; elle n'a été trouvée que dans des grottes où elle se nourrit sur les vampires ( Desmodus rotundus ).
Diagnosis by Isozyme Methods of Two Cryptic Species, Psychodopygus Carrerai and P. Yucumensis (Diptera: Psychodidae)1
(Oxford University Press, 1986) T. Caillard; Michel Tibayrenc; François Le Pont; Jean‐Pierre Dujardin; P. Desjeux; F.J. Ayala
Journal Article Diagnosis by Isozyme Methods of Two Cryptic Species, Psychodopygus Carrerai and P. Yucumensis (Diptera: Psychodidae) Get access T. Caillard, T. Caillard 2Instituto Boliviano de Biología de Altura (IBBA), Embajada de Francia, Casilla 824, La Paz, Bolivia. Send reprint requests to this address Search for other works by this author on: Oxford Academic PubMed Google Scholar M. Tibayrenc, M. Tibayrenc 3IBBA, and SSC ORSTOM, 70-74 route d'Aulnay, 93140 Bondy, France6Department of Genetics, University of California, Davis, California 95616, USA. Search for other works by this author on: Oxford Academic PubMed Google Scholar F. Le Pont, F. Le Pont 3IBBA, and SSC ORSTOM, 70-74 route d'Aulnay, 93140 Bondy, France Search for other works by this author on: Oxford Academic PubMed Google Scholar J.P. Dujardin, J.P. Dujardin 4IBBA, and Institut de Médecine tropicale “Prince Leopold,” 155 Nationalestraat, B-2000 Antwerpen, Belgium Search for other works by this author on: Oxford Academic PubMed Google Scholar P. Desjeux, P. Desjeux 5IBBA, and Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, France Search for other works by this author on: Oxford Academic PubMed Google Scholar F.J. Ayala F.J. Ayala 6Department of Genetics, University of California, Davis, California 95616, USA. Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Medical Entomology, Volume 23, Issue 5, 19 September 1986, Pages 489–492, https://doi.org/10.1093/jmedent/23.5.489 Published: 19 September 1986
First evidence of transmission of Leishmania (Viannia) lainsoni in a Sub Andean region of Bolivia
(Elsevier BV, 2002) Brigitte Bastrenta; R Buitrago; Fernando Regla Vargas; François Le Pont; M Torrez; María Flóres-Chávez; N Mita; Simone Frédérique Brénière
Geographic variation of metric properties within the neotropical sandflies
(Elsevier BV, 2004) Jean‐Pierre Dujardin; François Le Pont
Geographical versus interspecific differentiation of sand flies (Diptera: Psychodidae): a landmark data analysis
(Cambridge University Press, 2003) JP Dujardin; François Le Pont; Michel Baylac
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Human mixed infections of Leishmania spp. and Leishmania-Trypanosoma cruzi in a sub Andean Bolivian area: identification by polymerase chain reaction/hybridization and isoenzyme
(Instituto Oswaldo Cruz, Ministério da Saúde, 2003) Brigitte Bastrenta; N Mita; R Buitrago; Fernando Regla Vargas; María Flóres-Chávez; M Machane; Nina Yacsik; M Torrez; François Le Pont; Simone Frédérique Brénière
Parasites belonging to Leishmania braziliensis, Leishmania donovani, Leishmania mexicana complexes and Trypanosoma cruzi (clones 20 and 39) were searched in blood, lesions and strains collected from 28 patients with active cutaneous leishmaniasis and one patient with visceral leishmaniasis. PCR-hybridization with specific probes of Leishmania complexes (L. braziliensis, L. donovani and L. mexicana) and T. cruzi clones was applied to the different DNA samples. Over 29 patients, 8 (27.6%) presented a mixed infection Leishmania complex species, 17 (58.6%) a mixed infection Leishmania-T. cruzi, and 4 (13.8%) a multi Leishmania-T. cruzi infection. Several patients were infected by the two Bolivian major clones 20 and 39 of T. cruzi (44.8%). The L. braziliensis complex was more frequently detected in lesions than in blood and a reverse result was observed for L. mexicana complex. The polymerase chain reaction-hybridization design offers new arguments supporting the idea of an underestimated rate of visceral leishmanisis in Bolivia. Parasites were isolated by culture from the blood of two patients and lesions of 10 patients. The UPGMA (unweighted pair-group method with arithmetic averages) dendrogram computed from Jaccard's distances obtained from 11 isoenzyme loci data confirmed the presence of the three Leishmania complexes and undoubtedly identified human infections by L. (V.) braziliensis, L. (L.) chagasi and L. (L.) mexicana species. Additional evidence of parasite mixtures was visualized through mixed isoenzyme profiles, L. (V.) braziliensis-L. (L.) mexicana and Leishmania spp.-T. cruzi. The epidemiological profile in the studied area appeared more complex than currently known. This is the first report of parasitological evidence of Bolivian patients with trypanosomatidae multi infections and consequences on the diseases' control and patient treatments are discussed.
Identification of a major 72 kilodalton surface antigen in twelve isolates of Leishmania braziliensis braziliensis
(Elsevier BV, 1987) Dominique Legrand; P. Desjeux; François Le Pont; Simone Frédérique Brénière; Jean-Loup Lemesre; F Santoro; A Capron
Is<i>Lutzomyia serrana</i>(Diptera, Psychodidae, Phlebotominae) present in Ecuador ?
(EDP Sciences, 2004) JP Dujardin; François Le Pont; E. Maritnez
We describe a multivariate metric comparison of three sandfly species showing strong differences in size, which questions the geographical distribution of one of them. Two species are represented by a single population (L. robusta and L. guilvardae) and one by two populations (L. serrana). All of them belong to the series serrana (Diptera, Psychodidae, Phlebotominae). The morphometric data confirm that L. guilvardae is a distinct species. However, they suggest that L. robusta and L. serrana in Ecuador are the same taxon, and that it is distinguishable from the population of L. serrana in Bolivia. A multilocus enzyme electrophoresis analysis comparing L. serrana in Bolivia and L. robusta in Ecuador adds further evidence that these two populations are distinct species. Thus, our data seem to indicate that, in Ecuador, the population previously identified as L. serrana is actually the same species as the allopatric population previously identified as L. robusta. Accepting L. serrana in Ecuador as small-sized L. robusta, the resulting geographic distribution of this latter becomes in closer agreement with ecology and epidemiology.
Isozymic and metric variation in the Lutzomyia longipalpis complex
(Wiley, 1997) Jean‐Pierre Dujardin; E. M. Torrez; François Le Pont; David Abril Hervàs; David Sossa
Male Lutzomyia longipalpis of two types from Bolivia were compared using isozyme electrophoresis and wing morphometry. One sample (ex Chiflonkaka Cave, alt. 2800 m at Toro Toro, Charcas Province, Potosi Department) was 'two-spot' phenotype males (i.e. tergites III and IV with paired pale patches of pheromone glands), whereas two other locality samples (Apa Apa and Imanaco, Sud Yungas Province, La Paz Department) were one-spot male phenotype (only tergite IV with paired pale patches). Multilocus enzyme electrophoresis (using ACON, aGPD, GPI, IDH, MDH, ME, 6PGD, PGM, LAP and PEPB) found no difference between samples from adjacent hen houses at Apa Apa. Nei's standard genetic distance between one-spot samples from Apa Apa and Imanaco (5 km apart, 1500 m alt.) was 0.001-0.002, whereas the two-spot males from Toro Toro (800 km away) showed a genetic distance of 0.081 from the one-spot males (Apa Apa and Imanaco). This genetic distance is commensurate with speciation, but may simply be intraspecific differentiation due to 'isolation by distance'. For comparative wing morphometry, we included additional material of one-spot males from Bolivia (Guyabal, Sud Yungas, La Paz), Brazil, Colombia and Nicaragua. These three other country samples were assumed to be different sibling species in the complex Lutzomyia longipalpis (Lanzaro et al., 1993). Statistics were based on univariate and multivariate analysis. The comparison between size-in and size-free canonical variate analysis (CVA) indicated that the wing morphometric divergence between one-spot and two-spot Bolivian phenotypes was not size dependent and could have taxonomic significance.
[Leishmaniasis in Bolivia. IV. The dog in the cycles of leishmaniasis in Bolivia].
(National Institutes of Health, 1992) François Le Pont; S. Mollinedo; Jean Mouchet; P. Desjeux
In Bolivia, the dog is involved in the cycle of visceral leishmaniasis (Leishmania (Le.) chagasi) in the Yungas (alt. 1,000-2,000 m), and also in the cycle of cutaneous leishmaniasis (Le. (V.) braziliensis) in the Alto Beni (alt. 400-600 m). But it plays a different role in the two cycles. In the Yungas focus, it is the main reservoir of Le. (Le.) chagasi and the source of contamination for man. In the Alto Beni focus, it is only a "victim-host", like man, of Le. (V.) braziliensis; the reservoir of which is unknown. Wild mammals are very likely to be involved.
Leishmaniasis in Bolivia: V. Human strains of Leishmania (V.) braziliensis from the Department of Pando
(Instituto Oswaldo Cruz, Ministério da Saúde, 1989) J.M. Torres Espejo; Francine Pratlong; François Le Pont; Jean Mouchet; P. Desjeux; J. A. Rioux
Leishmaniose en Bolivie: IV. Le chien dans les cycles des leishmanioses en Bolivie
(Instituto Oswaldo Cruz, Ministério da Saúde, 1989) François Le Pont; S. Mollinedo; Jean Mouchet; P. Desjeux
En Bolivie le chien domestique est impliqué dans le cycle de la leishmaniose viscérale (Leishmania (Le.) chagasi) das les Yungas (alt. 1,000-2,000 m) et aussi dans le cycle de la leishmaniose tégumetaire (Le. (V.) braziliensis) dans l'Alto Beni (alt. 400-600 m). Mais il joue un rôle différent dans les deux cycles. Il est le prencipal résevoir, peut-être l'unique de Le. (Le.) chagasi dans les Yungas, et la source de contamination de l'home. Dans l'Alto Beni, il n'est seulement qu'une victime, comme l'home, de Le. (V.) braziliensis, dont le réservoir reste inconnu, les soupçons se portant sur des mammifères sauvages.
Lutzomyia neivai (Pinto, 1926) in Bolivia (Diptera, Psychodidae, Phlebotominae)
(Instituto Oswaldo Cruz, Ministério da Saúde, 1998) Carlos Brisóla Marcondes; François Le Pont; Ana Leuch Lozovei
International audience
Lutzomyia nuneztovari anglesi (Le pont & Desjeux, 1984) as a vector of Leishmania amazonensis in a sub-Andean leishmaniasis focus of Bolivia.
(American Society of Tropical Medicine and Hygiene, 1999) E. Martínez; François Le Pont; M Torrez; Jenny Telleria; Fernando Regla Vargas; Jean-Pierre Dujardin; JP Dujardin
Recently, a new Leishmania amazonensis focus was described in a sub-Andean region (1,450-2,100 meters above sea level) of Bolivia. In this area, three anthropophilic sandfly species were identified: Lutzomyia nuneztovari anglesi Le Pont & Desjeux, 1984, which represented 86-99% of the captures, Lu. galatiae Le Pont et al., 1998, and Lu. shannoni Dyar 1929. Only Lu. nuneztovari anglesi was found naturally infected by flagellates (16 of 1,715 females). Three Leishmania stocks were isolated and analyzed by isoenzyme electrophoresis at 11 loci. No significant isoenzymatic differences were demonstrated between them and 7 stocks isolated from patients from the same area, and previously characterized as L. amazonensis. Moreover, in a simplified protocol, the experimental infection of Lu. nuneztovari anglesi by L. amazonensis was successful in 92% of the surviving specimens. These data are discussed in relation to the Killick-Kendrick criteria. These results strongly suggest that Lu. nuneztovari anglesi is the vector of L amazonensis at Cajuata, Inquisivi, La Paz, Bolivia.