Browsing by Autor "Francisco Pellicer"

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Capsaicin or feeding with red peppers during gestation changes the thermonociceptive response of rat offspring
(Elsevier BV, 1996) Francisco Pellicer; Ofir Picazo; B. Gómez-Tagle; I. Roldán de la O
Cuando la conciencia corporal se enferma
(2002) Francisco Pellicer
De la nave de los locos a los fármacos de la razón
(2005) Francisco Pellicer
Waitlisted sensitised transplant recipients with HLA allele level antibodies to their own HLA antigen family are disadvantaged by current deficiencies in HLA typing for deceased donors. This is primarily because at time of organ allocation, HLA typing is provided at antigen level whereas solid phase assays provide allele level antibody definition. The gold standard for HLA allele typing is next generation sequencing (NGS), however time limitations with established NGS systems prevent NGS use for deceased donors. Instead, many labs use a real-time PCR (qPCR) antigen level result for deceased donors, which can disadvantage sensitised patients. Here, we compared assigning qPCR 2-field alleles to qPCR antigen level to determine the impact on virtual crossmatch (VXM) and discuss impact on donor-specific antibody (DSA) assignments. 244 consecutive deceased donors were HLA typed to allelic level by qPCR (LinkSeq SABR) and subsequently by NGS (One Lambda Alltype). The impact of qPCR allele assignments on potential DSA identification was investigated, by retrospectively investigating all 3904 VXMs, where recipient DSA assessments were assessed against donor HLA, was performed within the cohort. There was 96.3% concordance between qPCR and NGS for all allele level loci, with HLA-A; DQB1; and DPB1 having best agreement (99.4%, 98.4% and 99.4% respectively). Of the 3904 VXMs with qPCR allele assignment, there were 13 (<1%) occasions where the potential DSA assignment was impacted, with DQA1 having the most impact. Assigning alleles derived from qPCR to define unacceptable antigens for VXMs, can allow improved access to donor offers for sensitised patients by better defining alleles.
Electric stimulation of the cingulum bundle precipitates onset of autotomy induced by inflammation in rat
(Wiley, 1999) Francisco Pellicer; Alberto López-Ávila; Edgar Torres‐López
The purpose of this work was to test if electric stimulation of the cingulum bundle in animals subjected to a hindpaw inflammatory process precipitates the onset and enhances autotomy behaviour. Wistar rats were implanted with bipolar parallel electrodes in the boundary of the cingulum bundle. The inflammatory process was induced in all subjects by injection of carrageenan. The groups were: A, sham; B, implanted and stimulated 10 min daily for 7 days; C, implanted and stimulated 2 h daily, for 7 days. Both groups were injected with CAR 2 days after ending the stimulation period; and D, implanted and stimulated 10 min daily for 5 days, the first stimulation being simultaneous to CAR injection. Results show that 100% of the subjects in stimulated groups presented autotomy as compared with 66% in the sham group. A significant shortening of the onset and increased rates in autotomy were observed in experimental groups (B, C and D) as compared to the sham group. We did not find differences between groups B and C, but there was an increment of autotomy in group D when compared with both B and C groups. We conclude that it is possible to facilitate the onset and to increase the intensity of the autotomy triggered by the inflammatory process with cingulum bundle electrical stimulation. The results also suggest that a fundamental condition to the development of the autotomy in this model is the presence of the noxious inflammatory process. Copyright 1999 European Federation of Chapters of the International Association for the Study of Pain.
Nuevas aproximaciones al problema del miembro fantasma
(2001) Alberto López Avila; Francisco Pellicer
El presente trabajo analiza las recientes hipotesis acerca de la genesis del miembro fantasma (MF). Este trastorno se caracteriza por la sensacion que experimenta la mayoria de las personas a las que se les ha amputado un miembro o que han padecido la ablucion de un nervio, que consiste en seguir percibiendolo con un alto grado de realidad. Los primeros estudios acerca de la genesis del fenomeno se situan en un debate, es decir, se genera el fantasma en el munon del miembro amputado o es un constructo del sistema nervioso central. Una de las primeras hipotesis propone que la evocacion del MF se genera a partir de descargas ectopicas en el sitio de la lesion neural o neuroma. Las recientes corrientes de pensamiento situan a la genesis del MF como el resultado de un alto grado de encefalizacion, inclusive como parte de un proceso cognoscitivo afectivo con base en una representacion corporal localizada en la corteza y en diversos nucleos cerebrales. Una de las teorias propuesta es la de la neuromatriz, la cual establece que hay una red neuronal distribuida en varias areas del cerebro, que procesaria informacion paralela a la entrada somatosensorial y generaria una referencia geneticamente determinada del cuerpo. Otra de las hipotesis es la denominada cambio del mapa en lo concerniente a las sensaciones referidas, la cual sostiene que la genesis del MF se debe a la reorganizacion cortical rapida y precisa entre los sitios adyacentes a la representacion cortical del sitio desaferentado. Nuestro grupo ha generado una hipotesis que consiste en situar al detonador del MF en el sitio de la amputacion, y explica su persistencia mediante la activacion de un mecanismo fisiologico basico de potenciacion temporal y espacial en las areas cerebrales.
Self-mutilation in the Lesch-Nyhan syndrome: a corporalconsciousness problem? —a new hypothesis
(Elsevier BV, 1998) Francisco Pellicer; Ivette Buendía-Roldán; V.C. Pallares-Trujillo