Browsing by Autor "Gloria Rodrigo"

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A 5-methylcoumarin glucoside and a coumestan derivative from Mutisia orbignyana.
(Lund University, 2009) Yonny Flores; Gloria Rodrigo; Patricia Mollinedo; Björn Åkesson; Olov Sterner; Giovanna R. Almanza
The ethanolic extract of the aerial parts from Mutisia orbignyana afforded two major compounds: mutisifurocoumarin (1) and 5-methylcoumarine-4-β-glucoside (2). The completely assignment of 1H and 13C NMR data of compound (2) is presented for the first time as well as some reassignments of 13C NMR spectrum for compound (1), applying 2D NMR techniques. In addition, the antiproliferative effect on colon cancer cells (CaCo2) and the scavenging effect using the ABTS test were measured The results showed an interesting scavenging activity and a non proliferative effect on colon cancer cells./El extracto etanólico de las partes aéreas de Mutisia orbignyana presentó dos compuestos mayoritarios: mutisifurocumarina (1) y 5-metilcumarina-4-β-glucosilada (2). El asignamiento completo de RMN de 1H y 13C del compuesto (2) es presentado por primera vez, así como también algunos reasignamientos del espectro de RMN13C del compuesto (1), en ambos casos se aplico técnicas de RMN 2D. Además, se midió el efecto antiproliferativo sobre células cancerosas de colon (CaCo2) y el efecto como inhibidores de radicales libres usando la prueba ABTS, tanto de extractos como de compuestos puros. Los resultados muestran un interesante efecto antiradicalario en ABTS y un efecto no-proliferativo sobre células cancerosas de colon.
Alteraciones en el patrón de apoptosis de células precursoras de eritrocitos en mujeres postmenopáusicas con eritrocitosis patológica de la altura
(2000) Adelis Arias; Ricardo Amaru; Pilar Navia; Gloria Rodrigo; R Peñaloza; G Torrez; A Vázquez; H Cuevas
Análisis de la dinámica poblacional de células canserosas, mediante un modelo de Radiosensibilidad
(2019) Winder A Canezo-Gómez; Gloria Rodrigo; Gonzalo Marcelo Ramírez-Ávila
Anti-cancer stem cell activity of a sesquiterpene lactone isolated from Ambrosia arborescens and of a synthetic derivative
(Public Library of Science, 2017) Wendy Soria Sotillo; Rodrigo Villagomez; Sandra Smiljanic; Xiaoli Huang; Atena Malakpour; Sebastian Kempengren; Gloria Rodrigo; Giovanna R. Almanza; Olov Sterner; Stina Oredsson
New regimens are constantly being pursued in cancer treatment, especially in the context of treatment-resistant cancer stem cells (CSCs) that are assumed to be involved in cancer recurrence. Here, we investigated the anti-cancer activity of sesquiterpene lactones (SLs) isolated from Ambrosia arborescens and of synthetic derivatives in breast cancer cell lines, with a specific focus on activity against CSCs. The breast cancer cell lines MCF-7, JIMT-1, and HCC1937 and the normal-like breast epithelial cell line MCF-10A were treated with the SLs damsin and coronopilin, isolated from A. arborescens, and with ambrosin and dindol-01, synthesized using damsin. Inhibitory concentration 50 (IC50) values were obtained from dose-response curves. Based on IC50 values, doses in the μM range were used for investigating effects on cell proliferation, cell cycle phase distribution, cell death, micronuclei formation, and cell migration. Western blot analysis was used to investigate proteins involved in cell cycle regulation as well as in the NF-κB pathway since SLs have been shown to inhibit this transcription factor. Specific CSC effects were investigated using three CSC assays. All compounds inhibited cell proliferation; however, damsin and ambrosin were toxic at single-digit micromolar ranges, while higher concentrations were required for coronopilin and dindol-01. Of the four cell lines, the compounds had the least effect on the normal-like MCF-10A cells. The inhibition of cell proliferation can partly be explained by downregulation of cyclin-dependent kinase 2. All compounds inhibited tumour necrosis factor-α-induced translocation of NF-κB from the cytoplasm to the nucleus. Damsin and ambrosin treatment increased the number of micronuclei; moreover, another sign of DNA damage was the increased level of p53. Treatment with damsin and ambrosin decreased the CSC subpopulation and inhibited cell migration. Our results suggest that these compounds should be further investigated to find efficient CSC-inhibiting compounds.
Antioxidant and Antimutagenic Polyisoprenylated Benzophenones and Xanthones from <i>Rheedia acuminata</i>
(SAGE Publishing, 2011) Giovanna R. Almanza; Raúl Quispe; Patricia Mollinedo; Gloria Rodrigo; Ery Odette Fukushima; Rodrigo Villagomez; Björn Åkesson; Olov Sterner
Dichloromethane extract of the stem bark of Rheedia acuminata yielded three benzophenones with antioxidant activity, the new one named acuminophenone A (1), guttiferone K (2) and isoxanthochymol (3), along with the known xanthones formoxanthone C (4) and macluraxanthone (5). The structures were established through interpretation of their spectroscopic data, the stereochemistry of compounds (1) and (2) were resolved by experimental and computational experiments and their antioxidant activities were measured using the DPPH, ABTS and TEAC assays. The antioxidant results showed that metabolites 1, 4 and 5 had a better antioxidant activity than the reference compound quercetin. In addition, we evaluate the mutagenicity and antimutagenicity of the CH2Cl2 extract as well as of the free radical scavenger compounds 1, 4 and 5 by the AMES Salmonella/microsomal test. No mutagenicity was found in the CH2Cl2 extract using Salmonella typhimurium strains TA98, TA100, TA102, TA1537 and TA1538, with or without S9 metabolic activation. The pure compounds neither showed mutagenicity in TA 102 strain and the most important result was the strong reduction of mutagenic effect induced by hydrogen peroxide in S. typhimurium TA102, with or without S9, showed by the compounds 1 (more than 93%) and 4 (more than 88%) at 0.02 microg/plate.
Antiproliferative effects of curcuphenol, a sesquiterpene phenol
(Elsevier BV, 2010) Gloria Rodrigo; Giovanna R. Almanza; Yajun Cheng; Jiangnan Peng; Mark T. Hamann; Rui‐Dong Duan; Björn Åkesson
Damsina, coronopilina y santamarina, esquiterpenlactonas que manifiestan actividad citotóxica, capacidad de daño del ADN y capacidad de apóptosis en células cancerosas lineas a549, HeLa y Panc-1
(2018) Jaime P Iturri; Gloria Rodrigo
Gold Mining and Genotoxic Effects on Vicuñas: A Comparative Study of Buccal Cells and Lymphocytes
(Research Square (United States), 2024) Liz Romero; Gloria Rodrigo; Oscar Loayza; Robert B. Wallace
Abstract The vicuña (Vicugna vicugna) is a wild camelid native from South America, known for its highly valued fiber. In Bolivia, the Apolobamba protected area is a key area for vicuña conservation and Apolobamba’s indigenous communities sustainably harvest the fiber of wild vicuña. The vicuña is an important cultural and economic resource, as well as an indicator of ecosystem health. Over the last decade gold mining activities have increased in Apolobamba potentially causing high levels of mercury contamination, endangering the health of vicuñas, humans, and terrestrial ecosystems. This study used genotoxicity markers: micronuclei (MN) and nuclear abnormalities (NA) in buccal cells and lymphocytes of vicuñas in 13 vicuña management communities in Apolobamba. A mean frequency of 0.48% MN and 14.91% NA was found in buccal cells, and 0.32% MN and 57.13% NA in lymphocytes. A higher frequency of MN in buccal cells was expected as they are the first barrier to inhalation or ingestion of genotoxic agents. However, a higher frequency of NA in lymphocytes suggests a possible prevalence of damage. Furthermore, a gradient of MN frequency was observed consistently with mining activity, but mining may not be the only cause of this damage, as vicuñas are exposed to mixtures of environmental chemicals, including traces of microplastics and persistent organic pollutants that have been detected in the area too. These findings provide a baseline for future vicuña populations monitoring and can be used as bio monitors and sentinels of environmental pollution.
Inhibition of Protein Kinases AKT and ERK1/2 Reduce the Carotid Body Chemoreceptor Response to Hypoxia in Adult Rats
(Springer Nature, 2015) Pablo Iturri; Vincent Joseph; Gloria Rodrigo; Aïda Bairam; Jorge Soliz
Salinomicina comercial tiene actividad antiproliferativa sobre tres líneas celulares de cáncer en hipoxia natural a 3 600 msnm
(2025) Oscar M Rollano Peñaloza; Gloria Rodrigo; Jaime Pablo Iturri Soliz
Resumen La salinomicina es un compuesto prometedor como medicamento contra el cáncer debido a su acción antiproliferativa contra células madre de cáncer y su acción selectiva, es decir que no ataca células normales. Se ha mostrado resultados prometedores sobre la versión ultrapura del compuesto, pero aún no se ha evaluado la versión comercial, que actualmente es utilizada como suplemento alimenticio por veterinarios. Objetivo: Determinar la actividad antiproliferativa de la versión ultrapura y comercial de salinomicina sobre tres líneas celulares de cáncer. Métodos: Se realizo un estudio experimental in vitro transversal donde se evaluó el efecto antiproliferativo de dos versiones de salinomicina sobre las líneas Hela (células de cáncer cervicouterino), A549 (células de cáncer de pulmón) y MCF-7 (células de cáncer de mama) mediante el ensayo de citotoxicidad MTT y la evaluación de morfología celular apoptótica a las 48h y 72h de tratamiento. Además se evaluó las cantidades de salinomicina asimiladas por las líneas celulares a una altitud de 3 600 msnm. Resultados: Salinomicina en su versión comercial tiene una buena actividad antiproliferativa contra las líneas celulares HeLa, A549 y MCF-7. Ésta actividad es similar a la actividad del compuesto ultrapuro y al reportado por articulos previamente publicados. La asimilación de la salinomicina por las líneas celulares fue bastante baja, observandose que dentro de 72 horas de incubación todavia se observaba el 75% del compuesto en las concentraciones más altas. Los resultados sugieren que la versión comercial de salinomicina tiene el potencial de convertirse en una droga de bajo costo.