Browsing by Autor "Jenny Telleria"

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A new focus of cutaneous leishmaniasis due to Leishmania amazonensis in a Sub Andean region of Bolivia
(Elsevier BV, 1998) Eliana C. Martinez; François Le Pont; M Torrez; Jenny Telleria; Fernando Regla Vargas; M Muñoz; Simonne De Doncker; Jean‐Claude Dujardin; Jean‐Pierre Dujardin
Different Behavior of TwoTrypanosoma cruziMajor Clones: Transmission and Circulation in Young Bolivian Patients
(Elsevier BV, 1998) Simone Frédérique Brénière; Marie-France Bosseno; Jenny Telleria; Brigitte Bastrenta; Nina Yacsik; François Noireau; Jose-Luis Alcazar; Christian Barnabé; Patrick Wincker; Michel Tibayrenc
History of the Discovery of American Trypanosomiasis (Chagas Disease)
(Elsevier BV, 2010) Tânia Cremonini de Araújo-Jorge; Jenny Telleria; Jaime Ríos‐Dalenz
History of the discovery of the American Trypanosomiasis (Chagas disease)
(Elsevier BV, 2017) Tânia Cremonini de Araújo-Jorge; Jenny Telleria; Jaime Ríos Dalenz
Immune Response to Trypanosoma cruzi Shed Acute Phase Antigen in Children from an Endemic Area for Chagas' Disease in Bolivia
(Instituto Oswaldo Cruz, Ministério da Saúde, 1997) Simone Frédérique Brénière; Nina Yaksic; Jenny Telleria; Marie-France Bosseno; François Noireau; Patrick Wincker; Daniel O. Sánchez
A field study of the immune response to the shed acute phase antigen (SAPA) of Trypanosoma cruzi was carried out in the locality of Mizque, Cochabamba department, Bolivia. Schoolchildren (266), with an average of 8.6 +/- 3.6 years, were surveyed for parasitological and serological diagnosis, as well as antibodies directed against SAPA using the corresponding recombinant protein in ELISA. The antibodies against SAPA were shown in 82% of patients presenting positive serological diagnosis (IgG specific antibodies). The positive and negative predictive values were 0.88. Antibodies anti-SAPA were shown in 80.8% of the chagasic patients in the initial stage of the infection (positive IgM serology and/or positive buffy coat (BC) test) and in 81.4% of the patients in the indeterminate stage of the infection (positive IgG serology with negative BC and IgM tests). These results show that the anti-SAPA response is not only present during the initial stage of the infection (few months) but extends some years after infection.
Lutzomyia nuneztovari anglesi (Le pont & Desjeux, 1984) as a vector of Leishmania amazonensis in a sub-Andean leishmaniasis focus of Bolivia.
(American Society of Tropical Medicine and Hygiene, 1999) E. Martínez; François Le Pont; M Torrez; Jenny Telleria; Fernando Regla Vargas; Jean-Pierre Dujardin; JP Dujardin
Recently, a new Leishmania amazonensis focus was described in a sub-Andean region (1,450-2,100 meters above sea level) of Bolivia. In this area, three anthropophilic sandfly species were identified: Lutzomyia nuneztovari anglesi Le Pont & Desjeux, 1984, which represented 86-99% of the captures, Lu. galatiae Le Pont et al., 1998, and Lu. shannoni Dyar 1929. Only Lu. nuneztovari anglesi was found naturally infected by flagellates (16 of 1,715 females). Three Leishmania stocks were isolated and analyzed by isoenzyme electrophoresis at 11 loci. No significant isoenzymatic differences were demonstrated between them and 7 stocks isolated from patients from the same area, and previously characterized as L. amazonensis. Moreover, in a simplified protocol, the experimental infection of Lu. nuneztovari anglesi by L. amazonensis was successful in 92% of the surviving specimens. These data are discussed in relation to the Killick-Kendrick criteria. These results strongly suggest that Lu. nuneztovari anglesi is the vector of L amazonensis at Cajuata, Inquisivi, La Paz, Bolivia.
PCR-based diagnosis for Chagas' disease in Bolivian children living in an active transmission area: comparison with conventional serological and parasitological diagnosis
(Cambridge University Press, 1997) Patrick Wincker; Jenny Telleria; M. F. Bosseno; Micaela Cardoso; Patrícia Marques; Nina Yaksic; Christine Aznar; P. LIEGEARD; Mireille Hontebeyrie; François Noireau
A large field study has been performed in the Cochabamba region of Bolivia with the aim of comparing the polymerase chain reaction (PCR) with other diagnostic methods for Chagas' disease. The amplification of Trypanosoma cruzi-specific kinetoplast DNA sequences in blood samples was compared with classical serological methods, specific IgM detection and direct parasite visualization for 268 school children in a single village where Chagas' disease transmission is active. Of 113 children positive by classical serology or buffy coat examination, 106 were detected by PCR (sensitivity: 93.8%). We did not observe any significant difference of PCR sensitivity between initial (IgM and/or buffy coat positive) and indeterminate stage (only IgG positive) patients. Among the remaining 155 children unconfirmed as chagasic (who were either only IgM positive, IgG-, IgM-, and buffy coat-negative) only 1 case was PCR positive. This case may be due to DNA contamination, or to a very recent infection not detected otherwise, or to specific immune depression. These results show that PCR is a very sensitive parasitological test for Chagas' disease in active transmission regions. The future follow-up of the possibly infected patients who were only IgM-positive should clarify the interest of PCR and IgM tests in the detection of starting infections.
Polymerase chain reaction-based identification of New World Leishmania species complexes by specific kDNA probes
(Elsevier BV, 1999) Simone Frédérique Brénière; Jenny Telleria; Marie France Bosseno; R Buitrago; Brigitte Bastrenta; G Cuny; Anne‐Laure Bañuls; S. Brewster; Douglas C. Barker
Putative Reservoirs of Leishmania amazonensis in a Sub-andean Focus of Bolivia Identified by kDNA-Polymerase Chain Reaction
(Instituto Oswaldo Cruz, Ministério da Saúde, 1999) Jenny Telleria; Bosseno Mf; T. Tarifa; R Buitrago; E. Martínez; M Torrez; François Le Pont; Simone Frédérique Brénière
Sylvatic Triatomines (Hemiptera: Reduviidae) in Bolivia: Trends Toward Domesticity and Possible Infection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae)
(Oxford University Press, 1995) François Noireau; Marie-France Bosseno; Roxana Carrasco; Jenny Telleria; Fernando Regla Vargas; Clara Camacho; Nina Yaksic; Simone Frédérique Brénière
The risk of domestic transmission of Trypanosoma cruzi (Chagas) by sylvatic triatomines was assessed in an isolated area of the subandean region of Bolivia. None of the 390 residents examined had serological evidence of infection. Two sylvatic triatomine species, Eratyrus mucronatus (Stål) and Triatoma sordida (Stål), were found in houses and in peridomestic structures. The collection of nymphal instars of both species from some houses indicated possible domesticity. Microscopic examination of feces from 92 insects showed no parasites, and cultures from the guts of 30 insects were negative. Nevertheless, a polymerase chain reaction (PCR) test performed on the same fecal samples showed the presence of T. cruzi DNA in 19.1 and 12.5% of E. mucronatus and T. sordida, respectively. These 16 PCR-positive samples were hybridized with 2 T. cruzi-specific probes known from the domestic cycle in Bolivia (clones 20 and 39). At least 1 of these clones was identified in 7 bugs (5 E. mucronatus and 2 T. sordida). Moreover, no hybridization was observed with these probes in S E. mucronatus and 1 T. sordida samples that showed an amplified band by PCR. These data indicated that T. cruzi clones, genetically unrelated to clones 20 and 39, also were circulating in this area. Based on these results, the 2 sylvatic triatomine species encountered in Apolo should not be overlooked as possible local vectors of T. cruzi.
Trypanosoma cruzi:Study of the Distribution of Two Widespread Clonal Genotypes in BolivianTriatoma infestansVectors Shows a High Frequency of Mixed Infections
(Elsevier BV, 1996) Marie-France Bosseno; Jenny Telleria; Fernando Regla Vargas; Nina Yaksic; François Noireau; Annie Morin; Simone Frédérique Brénière