Browsing by Autor "Julio Alonso-Padilla"

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Development and Evaluation of a Three-Dimensional Printer–Based DNA Extraction Method Coupled to Loop Mediated Isothermal Amplification for Point-of-Care Diagnosis of Congenital Chagas Disease in Endemic Regions
(Elsevier BV, 2020) Diana P. Wehrendt; Julio Alonso-Padilla; Bo Liu; Lizeth Rojas Panozo; Silvia Rivera Nina; Lilian Pinto; Daniel Lozano; Albert Picado; Marcelo Abril; María‐Jesús Pinazo
Five-year serological and clinical evolution of chronic Chagas disease patients in Cochabamba, Bolivia
(Public Library of Science, 2023) Jimy Pinto; Malia Skjefte; Julio Alonso-Padilla; Daniel Franz Lozano Beltrán; Lilian Pinto; Aina Casellas; Mery Elena Arteaga Terrazas; Karen Galindo; Roxana Challapa Quechover; María Escobar Caballero
The study confirms that follow-up of benznidazole-treated T. cruzi-infected patients should be longer than five years to determine, with current tools, if they are cured. In terms of serological evolution, the single use of a total antigen ELISA might be a more reliable measure and suffice to address infection status, at least in the region of Bolivia where the study was done. Additional work is needed to develop a test-of-cure for an early assessment of drugs´ efficacy with the aim of improving case management protocols.
Host-Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers and Extracellular Vesicles
(Springer Science+Business Media, 2025) Berta Barnadas-Carceller; Dolors Tàssies; Irene Losada; Julio Alonso-Padilla; Joan Carles Reverter; Carmen Fernández-Becerra; María‐Jesús Pinazo
Molecular detection and parasite load of Trypanosoma cruzi in digestive tract tissue of Chagas disease patients affected by megacolon
(Elsevier BV, 2022) Lilian Pinto; Alejandro G. Schijman; Julio Alonso-Padilla; Daniel Lozano; Mary Cruz Torrico; Pietro Gamba; Margarita Torrez; Vania Lozada; Karina Cartagena; Jareth Sánchez
Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
(Public Library of Science, 2017) Karina E. Egüez; Julio Alonso-Padilla; Carolina Terán; Zenobia Chipana; Wilson García; Faustino Torrico; Joaquím Gascón; Daniel-Franz Lozano-Beltran; María‐Jesús Pinazo
Chagas disease is caused by the parasite Trypanosoma cruzi. It affects several million people, mainly in Latin America, and severe cardiac and/or digestive complications occur in ~30% of the chronically infected patients. Disease acute stage is mostly asymptomatic and infection goes undiagnosed. In the chronic phase direct parasite detection is hampered due to its concealed presence and diagnosis is achieved by serological methods, like ELISA or indirect hemagglutination assays. Agreement in at least two tests must be obtained due to parasite wide antigenic variability. These techniques require equipped labs and trained personnel and are not available in distant regions. As a result, many infected people often remain undiagnosed until it is too late, as the two available chemotherapies show diminished efficacy in the advanced chronic stage. Easy-to-use rapid diagnostic tests have been developed to be implemented in remote areas as an alternative to conventional tests. They do not need electricity, nor cold chain, they can return results within an hour and some even work with whole blood as sample, like Chagas Stat-Pak (ChemBio Inc.) and Chagas Detect Plus (InBIOS Inc.). Nonetheless, in order to qualify a rapidly diagnosed positive patient for treatment, conventional serological confirmation is obligatory, which might risk its start. In this study two rapid tests based on distinct antigen sets were used in parallel as a way to obtain a fast and conclusive Chagas disease diagnosis using whole blood samples. Chagas Stat-Pak and Chagas Detect Plus were validated by comparison with three conventional tests yielding 100% sensitivity and 99.3% specificity over 342 patients seeking Chagas disease diagnosis in a reference centre in Sucre (Bolivia). Combined used of RDTs in distant regions could substitute laborious conventional serology, allowing immediate treatment and favouring better adhesion to it.
Target product profile for a test for the early assessment of treatment efficacy in Chagas disease patients: An expert consensus
(Public Library of Science, 2020) Julio Alonso-Padilla; Marcelo Abril; Belkisyolé Alarcón de Noya; Igor C. Almeida; Andrea Angheben; Tania Araujo Jorge; Eric Chatelain; Mónica I. Esteva; Joaquím Gascón; Mario J. Grijalva
ISGlobal work is supported by the Departament d’Universitats i Recerca de la Generalitat de Catalunya, Spain (AGAUR; 017SGR00924) and by the Instituto de Salud Carlos III (ISCIII) RICET Network for Cooperative Research in Tropical Diseases (ISCIII; RD16/0027/0004 - PI1290) and FEDER. MJP research is supported by the Ministry of Health, Government of Catalonia (PERIS 2016-2010 SLT008/18/00132). ICA, JG, and FT are supported by the grant number U01AI129783 from the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH). ICA is also partly supported by the grant number 5U54MD007592 from the National Institute on Minority Health and Health Disparities (NIMHD), NIH. MCL and MCT were supported by ISCIII RICET grant RD16/0027/0005 - PI1290 and FEDER and by grants SAF2016-81003-R and SAF2016-80998-R from the Spanish “Programa Estatal I+D+i (MINECO)”. AA's work was supported by the Italian Ministry of Health “Fondi Ricerca Corrente - Linea 3, progetto 9” to IRCCS Sacro Cuore Don Calabria Hospital. JR was supported by CONACyT Fossis grant #261006. The Drugs for Neglected Diseases initiative (DNDi) is grateful to its donors, public and private, who have provided funding to DNDi since its inception in 2003. A full list of DNDi's donors can be found at http://www.dndi.org/donate/donors/. FIND is grateful to its donors, public and private, who have helped bring innovative new diagnostics for diseases of poverty. A full list of FIND’s donors can be found at: https://www.finddx.org/partners-donors/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Ultra-Rapid DNA Extraction Coupled with Loop-Mediated Isothermal Amplification for Point-of-Care Detection of Trypanosoma cruzi Infection in Heparinized Blood and Dried Blood Spots
(Springer Science+Business Media, 2025) Silvia A. Longhi; Lady J García-Casares; Arturo Muñoz-Calderón; Julio Alonso-Padilla; Alejandro G. Schijman