Browsing by Autor "Leonardo Roberto Rocabado-Villegas"

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • Thumbnail Image
    Item type: Item ,
    A novel glycoside hydrolase 43-like enzyme from <i>Clostridium boliviensis</i> is an endo-xylanase and a candidate for xylooligosaccharide production from different xylan substrates
    (American Society for Microbiology, 2024) Daniel Martín Salas-Veizaga; Leonardo Roberto Rocabado-Villegas; Javier A. Linares‐Pastén; Elísabet Eik Guðmundsdóttir; Guðmundur Ó. Hreggviðsson; María Teresa Álvarez Aliaga; Patrick Adlercreutz; Eva Nordberg Karlsson
    The genome of <i>Clostridium boliviensis</i> strain E-1 encodes a number of hypothetical enzymes, annotated as glycoside hydrolase-like but not classified in the Carbohydrate Active Enzyme Database (CAZy). A novel thermostable GH43-like enzyme is here characterized as an endo-β-xylanase of interest in the production of prebiotic xylooligosaccharides (XOs) from different xylan sources. <i>CbE1</i>Xyn43-l is a two-domain enzyme composed of a catalytic GH43-l domain and a CBM6 domain, producing xylotriose as main XO product. The enzyme has homologs in many related <i>Clostridium</i> strains which may indicate a similar function and be a previously unknown type of endo-xylanase in this evolutionary lineage of microorganisms.