Browsing by Autor "Liliana Muschietti"

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In Vitro Antiplasmodial Activity of Sesquiterpene Lactones from Ambrosia tenuifolia
(Hindawi Publishing Corporation, 2011) Valeria P. Sülsen; D. Gutierrez Yappu; Laura C. Laurella; Claudia Anesini; Alberto Giménez Turba; Virginia S. Martino; Liliana Muschietti
The in vitro antiplasmodial activity of Ambrosia tenuifolia organic extract and its isolated sesquiterpene lactones, psilostachyin and peruvin, has been evaluated against Plasmodium falciparum F32 and W2 strains. The cytotoxicity of both compounds was determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC(50)) of 0.3 μg/mL (1.1 μM) whereas psilostachyin showed activity on both strains (IC(50) = 0.6 (2.1 μM) and 1.8 μg/mL (6.4 μM)). Fifty percent cytotoxic concentration (CC(50)) values (48 h) were 6.8 μg/mL (24.3 μM) and 10.0 μg/mL (37.9 μM) for psilostachyin and peruvin, respectively.
In vitro Antiprotozoal Activity and Chemical Composition of Ambrosia tenuifolia and A. scabra Essential Oils
(SAGE Publishing, 2008) Valeria P. Sülsen; Silvia I. Cazorla; Fernanda M. Frank; Paola M. R. Di Leo Lira; Claudia Anesini; David Gutierrez-Yapu; Alberto Giménez-Turba; Arnaldo L. Bandoni; Emilio L. Malchiodi; Liliana Muschietti
The in vitro antimalarial, trypanocidal and leishmanicidal activities and the chemical composition of Ambrosia tenuifolia and A. scabra essential oils were investigated. The oils, obtained by hydrodistillation of the aerial parts, showed significant antimalarial activity against the chloroquine sensitive (F32) and the chloroquine resistant (W2) strains of Plasmodium falciparum with 50% inhibitory concentration (IC 50 ) values ranged from 2.8 to 7 μg/mL. Against Trypanosoma cruzi epimastigotes, only A. tenuifolia exerted slight activity (IC 50 = 59.7 μg/mL). None of the essential oils showed toxicity towards Leishmania mexicana. Cytotoxicity for murine T-lymphocytes was compared with the parasiticidal activity by using the selectivity index (SI) ratio. The chemical composition of A. tenuifolia and A. scabra essential oils was analyzed by GC-FID-MS. Thirty-eight and twenty-four compounds were identified in A. tenuifolia and A. scabra, respectively. The major constituents of A. tenuifolia oil were germacrene D (22.9%) and bicyclogermacrene (14.2%), while the oil of A. scabra contained limonene (22.0%), caryophyllene oxide (13.8%) and ( E)-caryophyllene (13.5%). This is the first time that the antiprotozoal activity of both oils and the chemical composition of A. scabra essential oil have been reported.