Browsing by Autor "Lina Maria Giraldo Deck"

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    New Microsatellite Markers for Two Sympatric Tinamou Species, the Ornate Tinamou (<i>Nothoprocta ornata</i>) and Darwin's Nothura (<i>Nothura darwinii</i>)
    (SAGE Publishing, 2016) Lina Maria Giraldo Deck; Jan Christian Habel; Manuel Curto; Martin Husemann; Sarah Sturm; Álvaro Garitano‐Zavala; Harald Meimberg
    Tinamous (Tinamidae) represent one of the most ancient living avian lineages but their life history traits are relatively unstudied. Here we identified microsatellite loci for two sympatric tinamou species, the Ornate Tinamou ( Nothoprocta ornata) and the Darwin's Nothura ( Nothura darwinii) from low coverage Illumina sequencing of genomic DNA. The experiment yielded a large number of candidate loci. We designed primers and tested them for successful amplification in 1 to 2 populations of the target species, tested for deviation from the Hardy–Weinberg equilibrium and the presence of null alleles, the levels of polymorphism and potential cross-amplification. All 30 and 24 loci amplified consistently, in the Ornate Tinamou and in Darwin's Nothura, respectively. In the Ornate Tinamou, 25 loci were polymorphic and in the Darwin's Nothura 12, with 2 to 14 alleles per locus in both species. The expected heterozygosity ranged from 0.054 to 0.917 in the Ornate Tinamou and from 0.044 to 0.908 in the Darwin's Nothura. 23 (40%) of 54 loci were successfully cross-amplified. These newly discovered, polymorphic microsatellite loci represent a valuable tool for future studies on social behaviour, parentage and genetic population structure in tinamous.
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    The small heart of the Ornate Tinamou is compatible with endothermy and flight but compromises aerobic metabolism and thermoregulation during recovery from exhaustive activity
    (Wiley, 2013) Jordi Altimiras; Lina Maria Giraldo Deck; Álvaro Garitano‐Zavala
    Tinamous are an ancient family of neotropical flying birds. Scant data from a few species show that they have a small heart so our aim was to characterize relative heart size of two species of the genus Nothoprocta and assess the physiological limitations associated with a small heart size. Relative heart size (0.24% for the Ornate Tinamou OT, a highland species and 0.28% for the Chilean Tinamou CT, a lowland species) was significantly smaller than high and lowland chickens (0.54% and 0.42% respectively), without evidence of right ventricular hypertrophy. Resting aerobic metabolism was 31% lower in OT than in highland chickens. When subjected to exhaustive activity, OT had elevated glucose and lactate levels suggesting a severe oxygen debt when exhausted. This was further shown as a significant drop in body temperature after an exhaustive bout. Finally heart rate while running on a treadmill at 3 km h −1 was 5% lower in OT, indicating that tinamous cannot compensate for the reduction in heart size with a faster heart rate. Altogether, we provide evidence that heart size is a phylogenetically conserved trait among tinamous and that the Ornate Tinamou cannot compensate aerobically for its small heart. Instead, it relies on anaerobic metabolism incurring in a large oxygen debt while exhausted. Supported by FORMAS Centre of Excellence in Animal Welfare Science and career grant from Linköpings universitet to JA.