Browsing by Autor "Loza-Murguia Manuel Gregorio"

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Biocontrol capacity of Beauveria brongniartii (Sacc.) and Metarhizium anisopliae (Metsch.) in the control of aphids Macrosiphum euphorbiae (Hemiptera: Aphididae)
(2017) Rojas-Gutiérrez René Leopoldo; Loza-Murguia Manuel Gregorio; Vino-Nina Nicolasa Lourdes; Teófilo Serrano-Canaviri
Aiming to develop alternative technologies in controlling aphids (Macrosiphum euporviae), the biocontrol ability of entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae evaluated. Par study native fungi of 3 parts of the central highlands (Achacachi , Viacha and Patacamaya) , which is isolated from the mummified insects and purified in the laboratory, the isolates were as fungi of the genus Beauveria result and Metarhizium were collected. Of these isolates its initial concentration was removed and all were adjusted to the minimum concentration lodged in one of the isolates was 9.5 x 10⁴conidia/mL at these concentrations the virulence test was performed for 144 hours , where isolated 2BbVch , 1BbAch , 3BbPtm 1MtAch and produced more than 50 % mortality , indicating that the genus Beauveria isolates proved to be better . With isolates that demonstrated improved mortality were subjected to a second test determining the lethal time 50 and 80 ( LT50 ) ( TL80 ) where 2BbVch isolated showed a lower time control 50 and 80% with 78 aphids 1 and 123.8 hours respectively . 50 and the lethal concentration 80 ( IC50 ) was determined, (IC80), where it was observed that the isolated 1BbAch 2BbVch and require minimal concentrations of 2.5 × 10⁴ and 5.1 x 10⁴ conidia/mL, to control 50% of aphids as a concentration of 1.3 x 10⁵ to 1.8 x10⁵ conidia/mL for 80 % control of aphids.
Determination the behavior of the curve lactation Mestizo and dairy cattle Altiplano of the Province Omasuyos Department of La Paz-Bolivia
(2016) Apaza-Huallpa Yesenia; Loza-Murguia Manuel Gregorio; Rojas-Pardo Abel; Achu-Nina Cristóbal
The present research work was conducted in eight communities, Municipality of Achacachi Omasuyos Province, with the following objectives: i) calculate the parameters that describe the lactation curve in cows with and without stable. 20 crossbred cows were selected (Holstein crosses with Criollo). 14 and 6 had stable without stable, all were in the last third of gestation to give birth in the months of January month end, February and March earlier this month, milk production was determined from birth until natural drying, their diet was based on native grasslands, pastures of alfalfa, oats and barley (Green, hay and straw). The analysis of the results obtained have on average (a = 4.248, b = 0.348 and c = 0.006 different Ticona (2001), possibly because the average current milk production is (10 kg / day). The average milk production to 305 days of lactation (DL - 305), in cows with stable was 3242.68 kg with an average daily production of 10.6 kg; while the average milk production to 305 days of lactation (PL - 305) in cows without stable was 2621.87 kg, with an average production of 8.6 kg per day. The model proposed by Wood (1967), It was adequate in determining the parameters that define the lactation curve in crossbred cows in the highlands, and to estimate milk production in the town of Achacachi, Omasuyos province, department of La Paz.
Effect of six energetic substrates on the time of sperm viability of the semen of Alpaca (Vicugna pacos L.)
(2017) Paxipatty-Quispe Víctor Abdón; Loza-Murguia Manuel Gregorio; Vino-Nina Nicolasa Lourdes; Felipe Chura-Limachi; Gonzales-Vargas Víctor Efraín; Delgado-Callisaya Pedro Ángel
The research was carried out in the Laboratory of Reproductive Biotechnology of the UAC Tiahuanacu, Ingavi province of the Department of La Paz, dependent of the Bolivian Catholic University, whose objective was to evaluate the effect of six energetic substrates on the sperm viability time of the alpaca semen (Vicugna pacos L.). The collected semen was diluted and stored in six different energy substrates (TCG, TCAP, TCF, TG, TAP and TF), a T ° of 35 ºC, evaluating every 2 hours, using the Completely Random Design (P < 0.05). Obtaining a mean volume of semen 1.5 ± 0.7 mL, filament 5.1 ± 1.5, pH 7.2 ± 0.5, motility and vitality of 89.0 and 95.0% and concentration 82.0x106 Epz / mL. The energetic substrate (SE) TCG managed to maintain a motility of 61.0 and 66.0% of vitality preserved for 8 hours, in that sense it is recommended to use the SE TCG, which maintained for a longer time (hours) the viable spermatozoa for LAI.
Evaluation of biol`s effect on fourteen accession of native potato (Solanum ssp.) at the experimental station kallutaca
(2017) Cristina Rodríguez Pastor; Loza-Murguia Manuel Gregorio; Sherman D. Nelson; G. Jorge; Mamani-Pati Francisco; Félix Marza-Mamani; Gutiérrez-Gonzáles Diego Eddy
In Bolivia the productive potato regions, you characterize for an extensive agriculture in blankets (of traditional form), of car subsistence and not much diversified, the little properties for the cultivation of potato possess the greater part of the productive, and they locate at little plots of land that annually they go rotating. The present research work has the objective to make a comparison and to characterize fourteen accessions of native potato of germoplasma's collection, of Agronomic Ingeniería's race, (UPEA). The study carried completion at Kallutaca's experiment station itself, becoming established on the basis of a Design Bloques Complete at random (DBCA) with three repetitions each accession, the second factor is diligent biol's dose, considered himself like a stimulant compound fito, for cultivations, where increments in the capability of photosynthesis and the production allow principal. Whose moral values place between 2.0 2.6% themselves (% in dry material) and that they yielded 3.3 %'s concentration of total N to 3.7%. The analysis of microcomputer Hierro's (132 ppm), Manganeso's, Cobre's and Zinc's presence with 12.1 and 6 ppm detected nutrient in the obtained effluent. Accessions Pole, Sacampaya, Sani imilla, Janq'o, collide, Runic Bola, Q'aysalla and Laramq'aysa had bigger development, the same the remainders have bigger performances they did not have the same incidence. This answer is due to a positive effect of the biol on the performance of tubers in accordance with dose of diligent biol the accessions of native potato where 1 with the application of dose of 25 % of Biol obtained to 10 t.h-1 an equivalent yield.
Filamentous soil fungi comunities on Kiphakiphani agroesosistems, Choquenaira-Viacha Municipaly
(2017) Pacasa-Quisbert Fernando; Loza-Murguia Manuel Gregorio; Bonifacio-Flores Alejandro; Vino-Nina Nicolasa Lourdes; Teófilo Serrano-Canaviri
Soil fungi play important roles in ecosystems and these populations are closely related to soil type, climatic characteristics, other microorganisms and plant communities. People’s activities, mainly agriculture alter their habitat and therefore affect fungi diversity and functions on soil. The effects of soil use type (TUS), native vegetation type (TVN) and crop type (TC) on colony forming units (CFU), number of spe-cies and physical, chemical and biological parameters was assessments in the present work in Plurination-al State of Bolivian´s highland agroecosystem. In addition, the diversity and number of filamentous fungi were related to physical and chemical soil parameters (pH, texture, organic matter), The fungi were de-scribed macro- and microscopically and were taxonomically identified. The TUS, TVN and TC influenced differently on the pH, soil texture and soil organic matter (SOM). The TUS and TC significantly affected the CFU´s and number of species. CFU´s were high in crop potato and number of species in Trifolium sp., 44 species were isolated, predominating genera of Penicillium and Alternaria. The rela-tionships between SOM were non-significant and weak (r2 = 32.1, p> 0.05), pH, significant and strong positive relation (r2 = 47.7, p <0.05) and soil texture vs CFU´s no significant and unrelated (r2 = 8.6, p> 0.05). The results suggest that the soil use type affect in soil properties, plant diversity and community fungi. Additionally, it is necessary to investigate other parameters that influence the microbial community in the agroecosystem.
Microscopic evaluation of semen characteristics llama (Lama glama) cryopreserved two dilutors
(2016) Laruta-Limachi Felicidad; Loza-Murguia Manuel Gregorio; Delgado-Callisaya Pedro Ángel
The present study was conducted at the Experimental Station Kallutaca, under the race Veterinary Medicine and Animal Husbandry of the UPEA, located in the province of Los Andes Department of La Paz at 3900 meters, whose objective was to evaluate the microscopic features semen llama (Lama glama) cryopreserved two diluters. 15 ejaculates were processed using llamas electroejaculation 12 players 4 years old on average. The test was part of a design of random blocks (DBA). The treatments were: T1. Andromed and T2. Tris fructose citric-acid-yolk-glycerol. The variables evaluated were: sperm motility (%), sperm vitality (%) and morphological abnormalities (%). By comparing the sperm variables in the post-thawed (cryopreserved) significant differences (P<0.05) between dilutors for sperm motility (%), sperm vitality (%) and morphological abnormalities (%), average values obtained are found and standard deviation: T1. 0%; T2. 17.03±1.83%, T1. 3.80±0.40%, T2. 20.13±2.06% and T1. 8.40±0.64% and T2. 10.17±0.22% respectively for the dilutor Andromed Tris-citric acid and fructose-yolk-glycerol. The results of cryopreserved semen diluters both proved to be lower than those obtained in fresh semen, because the semen quality decreases as time passes cryopreservation.
Oestrus ovis (Diptera: Oestridae) an important ectoparasite in sheep of four cantons of the municipality of Sorata province Larecaja, department of La Paz
(2017) Choque-Fernández Graciela Cristina; Loza-Murguia Manuel Gregorio; Vino-Nina Nicolasa Lourdes; Coria-Conde Luis Alfredo
The study of Oestrosis in sheep was carried out in the cantons of Sorata, Obispo Bosque, Laripata and Ilabaya, belonging to the Municipality of Sorata, under the agreement made with the institution Departmental Agricultural and Livestock Service (SEDAG) between August and September 2002, with 164 heads of sheep out of which 151 heads were Oestrus ovis, 13 were negative, taking into account the factors canton, sex, age and number of larvae per animal. The prevalence of Oestrosis in Sorata was 39% and by corner Obispo Bosque 28%, Ilabaya and Laripata 18% and 15% respectively. The analysis of variance to quantify the number of larvae per canton did not present significant differences (P> 0.005) as well as for the sex and age factor, which means that the canton Sorata, Obispo Bosque, Laripata and Ilabaya have equal % of Oestrosis for The age factor is not significant difference between females and males nor groups of age 1 and 2. Analysis of variance to determine the differences in the number of larvae per canton, sex, age and larval stage also does not exist significant differences, however, considering (P <0.005) because there are more larvae in the L-1 stage than L-2 and L-3. In this sense, Oestrosis affects all the cantons under study without distinction of age groups, sex, however varying in the presence of different larval stages, where L-1 predominates.
Una mirada de la revista desde su nacimiento
(2017) Loza-Murguia Manuel Gregorio
Desde que nace Journal of the Selva Andina Research Society (JSARS) un 25 de septiembre de 2009, llegamos a publicar 8 volúmenes y 14 números, en su inicio la revista se incorpora al formato Open Journal Systems (OJS), fue en el portal http://www.ojsbolivia.org que salimos por primera vez en octubre del año 2010, los primeros autores que publicaron nos felicitaron por el gran logro, pero fue su inicio, ahora se venía el gran reto, mantener la revista y para ello se tuvo que indexar en LATINDEX, quienes aceptaron y estamos visibles en su portal, luego fue en DIALNET, y REDALYC, para luego proseguir en el Programa SciELO (Scientific Electronic Library Online), finalmente en DOAJ. Ahora buscamos la indexación en otros portales que en el tiempo nos permitan hacer que la revista este visible en Índex Medicus/MEDLINE, sé que es una tarea anhelada pero estamos en ese proceso. Como Director-Editor en Jefe el reto que nos hemos trazado es difícil pero no imposible, dependemos de un Comité Editorial de Excelencia, que sin su participación en cuanto a la revisión de los trabajos que nos envían los autores y que buscan que su artículo este editado en Journal of the Selva Andina Research Society, es de gran responsabilidad, desde la recepción hasta la edición dependemos de un grupo de trabajo, que se identifica con la revista. La política de difundir la revista en el país y fuera, abriendo a profesionales hispano-parlantes en Latino America, España, y E.U.A., en Bibliotecas Universitarias, Centros e Institutos de Investigación, Estudiantes de Postgrado y Pregrado en las áreas de la revista ha hecho que los artículos publicados sean motivo de su lectura y en gran medida la referencia del artículo que les sea afín. Ahora y después de cerca ocho años de trabajo silencioso, recibimos las contribuciones de diferentes países, lo que quiere decir que la revista está siendo visitada y evaluada, además la confianza por parte de los Investigadores, Docentes Universitarios, Estudiantes de Pregrado, Postgrado, Servidores Públicos de Hospitales, ONG´s, Fundaciones, envían sus trabajo, lo que nos llena de gran orgullo y confianza. Ahora estamos editando el Vol 8 no 2. 2017, que recorrido, y seguiremos siendo visibles porque nuestro objetivo es hacer visible al país y los países amigos, que con sus contribuciones hacen crecer este proyecto. Estamos ahora en proceso de logar el DOIs (Digital Object Identifiers), y a partir del Vol 9 no1 2018 esperamos contar con este código. Finalmente, hacemos extensiva la invitación para que envíen sus trabajos de investigación y sean motivo de publicación previa evaluación por pares.