Browsing by Autor "Margarita Bisio"

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    Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
    (Frontiers Media, 2023) María‐Jesús Pinazo; Colin Forsyth; Constanza Lopez-Albízu; Margarita Bisio; Adriana González Martínez; Laura C. Bohórquez; Jimy Pinto; Israel Molina; Andrea Marchiol; Rafael Herazo
    <i>Trypanosoma cruzi</i> infection is diagnosed by parasitological, molecular, and serological tests. Molecular methods based on DNA amplification provide a more sensitive alternative to classical parasitological techniques for detecting evidence of <i>T. cruzi</i> parasitemia, and are the preferred tests for congenital and oral transmission cases and parasite reactivation in chronically infected immunosuppressed individuals. In newborns at risk of vertical transmission, simplified diagnostic algorithms that provide timely results can reduce the high follow-up losses observed with current algorithms. Molecular methods have also proved useful for monitoring <i>T. cruzi</i> infection in solid organ transplantation recipients, regardless of host immune status, allowing parasite detection even before symptom manifestation. Furthermore, in the absence of other biomarkers and a practical test of cure, and given the limitations of serological methods, recent clinical guidelines have included polymerase chain reaction (PCR) to detect therapeutic failure after antiparasitic treatment in chronically infected adults. Increasing evidence supports the use of molecular tests in a clinical context, given the improved sensitivity and specificity of current assays - characteristics which largely depend on epidemiological factors and genetic and antigenic variability among <i>T. cruzi</i> strains. Further development and registration of commercial PCR kits will improve the use of molecular tests. We discuss the attributes of PCR and other molecular tests for clinical management in people with <i>T. cruzi</i> infection.
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    International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
    (Public Library of Science, 2011) Alejandro G. Schijman; Margarita Bisio; Liliana Orellana; Mariela Sued; Tomás Duffy; Ana María Mejía‐Jaramillo; Carolina Cura; Frederic Auter; Vincent Véron; Yvonne Qvarnström
    This study represents a first crucial step towards international validation of PCR procedures for detection of T. cruzi in human blood samples.
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    Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples
    (Public Library of Science, 2015) Carolina Cura; Tomás Duffy; Raúl Horacio Lucero; Margarita Bisio; Julie Péneau; Matilde Jiménez‐Coello; Eva Muñoz; María-José Giménez; Edward Valencia Ayala; Sonia A. Kjos
    Typing is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production.