Browsing by Autor "Marie France Bosseno"

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Current spread of Triatoma infestans at the expense of Triatoma sordida in Bolivia
(Instituto Oswaldo Cruz, Ministério da Saúde, 1996) François Noireau; Simone Frédérique Brénière; Lucyla Cardozo; Marie France Bosseno; Fernando Regla Vargas; Carlos Peredo; Marlon Medinacelli
Ecogenetics of Triatoma sordida and Triatoma guasayana (Hemiptera: Reduviidae) in the Bolivian Chaco
(Instituto Oswaldo Cruz, Ministério da Saúde, 1999) François Noireau; Teresa de Jesús Molina Gutiérrez; Rosmary Flores; Simone Frédérique Brénière; Marie France Bosseno; Cristina Wisnivesky‐Colli
Triatoma guasayana and two putative cryptic species pertaining to T. sordida complex (named groups 1 and 2) occur in sympatry in the Bolivian Chaco. Using multilocus enzyme electrophoresis and subsequent genetic analysis, our work assesses their population distribution and dispersal capacity in domestic, peridomestic, and silvatic environments. Our collections by light trap in the silvatic environment indicated a predominance of T. guasayana and T. sordida group 2 and a lesser abundance of T. sordida group 1 ( pound 10% of the total of captures). Their similar distribution in two silvatic areas 80 km apart supports the hypothesis of their homogeneous dispersal through the Bolivian Chaco. The distribution of T. guasayana and T. sordida groups 1 and 2 was similar between silvatic environment and peridomestic ecotopes where 25% of positive places was occupied by two or three species. Bromeliads were confirmed as favorable shelter for T. guasayana but were free of T. sordida. T. sordida group 1 and to a lesser extent T. guasayana would be more invasive vectors for houses than T. sordida group 2. The spatial partition in the three species sampled in two distant sites suggested a reduced dispersive capacity.
Integrate Study of a Bolivian Population Infected by Trypanosoma cruzi, the Agent of Chagas Disease
(Instituto Oswaldo Cruz, Ministério da Saúde, 2002) Simone Frédérique Brénière; Marie France Bosseno; François Noireau; Nina Yacsik; Pascale Liegeard; Christine Aznar; Mireille Hontebeyrie
A cross section of a human population (501 individuals) selected at random, and living in a Bolivian community, highly endemic for Chagas disease, was investigated combining together clinical, parasitological and molecular approaches. Conventional serology and polymerase chain reaction (PCR) indicated an active transmission of the infection, a high seroprevalence (43.3%) ranging from around 12% in < 5 years to 94.7% in > 45 years, and a high sensitivity (83.8%) and specificity of PCR. Abnormal ECG tracing was predominant in chagasic patients and was already present among individuals younger than 13 years. SAPA (shed acute phase antigen) recombinant protein and the synthetic peptide R-13 were used as antigens in ELISA tests. The reactivity of SAPA was strongly associated to Trypanosoma cruzi infection and independent of the age of the patients but was not suitable neither for universal serodiagnosis nor for discrimination of specific phases of Chagas infection. Anti-R-13 response was observed in 27.5% only in chagasic patients. Moreover, anti-R13 reactivity was associated with early infection and not to cardiac pathology. This result questioned previous studies, which considered the anti-R-13 response as a marker of chronic Chagas heart disease. The major clonets 20 and 39 (belonging to Trypanosoma cruzi I and T. cruzi II respectively) which circulate in equal proportions in vectors of the studied area, were identified in patients' blood by PCR. Clonet 39 was selected over clonet 20 in the circulation whatever the age of the patient. The only factor related to strain detected in patients' blood, was the anti-R-13 reactivity: 37% of the patients infected by clonet 39 (94 cases) had anti-R13 antibodies contrasting with only 6% of the patients without clonet 39 (16 cases).
Low probability of transmission of Trypanosoma cruzi to humans by domiciliary Triatoma sordida in Bolivia
(Oxford University Press, 1997) François Noireau; Simone Frédérique Brénière; Juan C. Ordóñez; L. Cardozo; W. Morochi; T. Gutierrez; Marie France Bosseno; S. Garcia; Fernando Regla Vargas; Nina Yaksic
The role of Triatoma sordida in the domestic transmission of Trypanosoma cruzi was assessed in 7 rural localities in Velasco Province, Department of Santa Cruz, Bolivia. Tri. sordida, the only triatomine species identified in these localities, was found inside 58.0% of houses but not in large numbers (3.1 bugs per infested house on average). A total of 220 faecal samples from domiciliary bugs was examined microscopically and by the polymerase chain reaction for the presence of trypanosomes: 21.4% were infected. Analysis of blood meals of domiciliary Tri. sordida showed that humans were the commonest host (70.4%), followed by chickens and dogs. Four of 418 persons tested were seropositive for Tryp. cruzi. Only 2 of a second group of 62 persons living in dwellings infested by Tri. sordida were seropositive. Tryp. cruzi infection was demonstrated in dogs and domestic rats. Three other species of small mammals were found to be infected with trypanosomes. In our study area, domestic Tri. sordida are mainly incriminated in the transmission of Tryp. cruzi to synanthropic animals, whereas transmission to humans is very rare. The presence in houses of small populations of Tri. sordida infected with Tryp. cruzi is therefore currently insufficient for this insect to constitute a major epidemiological risk factor.
Polymerase chain reaction-based identification of New World Leishmania species complexes by specific kDNA probes
(Elsevier BV, 1999) Simone Frédérique Brénière; Jenny Telleria; Marie France Bosseno; R Buitrago; Brigitte Bastrenta; G Cuny; Anne‐Laure Bañuls; S. Brewster; Douglas C. Barker
Risk of transmission of Trypanosoma cruzi by wild Triatoma infestans (Hemiptera: Reduviidae) in Bolivia supported by the detection of human blood meals
(Elsevier BV, 2013) Nelly Lilian Rosio Buitrago; Marie France Bosseno; Etienne Waleckx; Philippe Brémond; Pablo Vidaurre; Faustine Zovéda; Simone Frédérique Brénière