Browsing by Autor "Marie-France Bosseno"

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Circulation and behaviour of two major clones of Trypanosoma cruzi in Bolivian cycles
(Centre National de la Recherche Scientifique, 1996) Simone Frédérique Brénière; Marie-France Bosseno; François Noireau
International audience
Combination of cytochrome b heteroduplex-assay and sequencing for identification of triatomine blood meals
(Elsevier BV, 2011) Rosio Buitrago; Stéphanie Depickère; Marie-France Bosseno; Edda Siñani Patzi; Etienne Waleckx; Renata Salas; Claudia Aliaga; Simone Frédérique Brénière
Different Behavior of TwoTrypanosoma cruziMajor Clones: Transmission and Circulation in Young Bolivian Patients
(Elsevier BV, 1998) Simone Frédérique Brénière; Marie-France Bosseno; Jenny Telleria; Brigitte Bastrenta; Nina Yacsik; François Noireau; Jose-Luis Alcazar; Christian Barnabé; Patrick Wincker; Michel Tibayrenc
Distribucion de los clones de Trypanosoma cruzi en vectores secundarios en Bolivia
(1999) Simone Frédérique Brénière; Marie-France Bosseno; W. Morochi; F. Vargas; François Noireau
Expansion actual de Triatoma infestans a costa de Triatoma sordida en Bolivia
(1999) François Noireau; Simone Frédérique Brénière; L. Cardozo; Marie-France Bosseno; F. Vargas; Carlos Peredo; Marlon Medinacelli
First Report of Widespread Wild Populations of Triatoma infestans (Reduviidae, Triatominae) in the Valleys of La Paz, Bolivia
(American Society of Tropical Medicine and Hygiene, 2010) Rosio Buitrago; Pablo Vidaurre; Faustine Zovéda; Simone Frédérique Brénière; Etienne Waleckx; Renata Salas; Marie-France Bosseno; François Noireau; Elio Mamani
Wild populations of Triatoma infestans, the main vector of Chagas disease in the Southern Cone countries, may be involved in reinfestation of human dwellings, limiting the success of vector-control campaigns in Bolivia. Knowledge of the distribution of these populations remains incomplete. We report here the detection of T. infestans wild populations in large areas in the department of La Paz, Bolivia. Among 18 sylvatic areas investigated, 17 were positive with T. infestans specimens. The infection rate of captured T. infestans with Trypanosoma cruzi was 85.7% in adult specimens. These results expand the geographical distribution of wild populations of T. infestans; it may be distributed throughout the Inter-Andean Dry Forest eco-region of Bolivia. The current information allows us to propose the hypothesis that a sylvatic origin of the reinfestation is located in the valleys of La Paz.
High correlation between Chagas' disease serology and PCR-based detection of<i>Trypanosoma cruzi</i>kinetoplast DNA in Bolivian children living in an endemic area
(Oxford University Press, 1994) Patrick Wincker; Marie-France Bosseno; Constança Britto; Nina Yaksic; Micaela Cardoso; Carlos MÃ©dicis Morel; Simone FrÃ©dÃ©rique BreniÃ ̈re
The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas' disease diagnosis in Bolivia.
Immune Response to Trypanosoma cruzi Shed Acute Phase Antigen in Children from an Endemic Area for Chagas' Disease in Bolivia
(Instituto Oswaldo Cruz, Ministério da Saúde, 1997) Simone Frédérique Brénière; Nina Yaksic; Jenny Telleria; Marie-France Bosseno; François Noireau; Patrick Wincker; Daniel O. Sánchez
A field study of the immune response to the shed acute phase antigen (SAPA) of Trypanosoma cruzi was carried out in the locality of Mizque, Cochabamba department, Bolivia. Schoolchildren (266), with an average of 8.6 +/- 3.6 years, were surveyed for parasitological and serological diagnosis, as well as antibodies directed against SAPA using the corresponding recombinant protein in ELISA. The antibodies against SAPA were shown in 82% of patients presenting positive serological diagnosis (IgG specific antibodies). The positive and negative predictive values were 0.88. Antibodies anti-SAPA were shown in 80.8% of the chagasic patients in the initial stage of the infection (positive IgM serology and/or positive buffy coat (BC) test) and in 81.4% of the patients in the indeterminate stage of the infection (positive IgG serology with negative BC and IgM tests). These results show that the anti-SAPA response is not only present during the initial stage of the infection (few months) but extends some years after infection.
New insights on the Chagas disease main vector Triatoma infestans (Reduviidae, Triatominae) brought by the genetic analysis of Bolivian sylvatic populations
(Elsevier BV, 2011) Etienne Waleckx; Renata Salas; Nerida Huamán; Rosio Buitrago; Marie-France Bosseno; Claudia Aliaga; Christian Barnabé; Roberto Rodríguez; Faustine Zovéda; Marcelo Monje
POLYMERASE CHAIN REACTION DETECTION AND SEROLOGIC FOLLOW-UP AFTER TREATMENT WITH BENZNIDAZOLE IN BOLIVIAN CHILDREN INFECTED WITH A NATURAL MIXTURE OF TRYPANOSOMA CRUZI I AND II
(American Society of Tropical Medicine and Hygiene, 2006) María Flóres-Chávez; Marie-France Bosseno; Brigitte Bastrenta; JOSE-LOUIS ALCAZAR DALENZ; Mireille Hontebeyrie; Susana Revollo; Simone Frédérique Brénière
Thirty-five Bolivian children (5-10 years of age) seropositive for infection with T. cruzi underwent specific chemotherapy with benznidazole. Before treatment, 57.1% had a positive parasitologic diagnosis. Some patients presented an early conversion by polymerase chain reaction of blood samples, while others were still positive four and seven months after the end of the treatment, which indicated an absence of parasite clearance. Strain typing showed that most patients were infected by a mixture of clones I and II of T. cruzi. Serologic conversion in conventional tests and antibodies to shed acute-phase antigen were observed in two and four patients, respectively. For the other patients, the average rate of antibody decay was half the initial rate. The parasitologic and serologic data indicated that chemotherapy acts throughout the course of infection in a long-lasting process in which the decrease of specific antibody production is related to the reduction of the live parasite load.
Population genetic structure of Meccus longipennis (Hemiptera, Reduviidae, Triatominae), vector of Chagas disease in West Mexico
(Elsevier BV, 2011) Simone Frédérique Brénière; Etienne Waleckx; Ezequiel Magallón-Gastélum; Marie-France Bosseno; Xavier Hardy; Cyrille Ndo; Felipe Lozano-Kasten; Christian Barnabé; Pierre Kengne
Selection of Trypanosoma cruzi clonal genotypes (clonet 20 and 39) isolated from Bolivian triatomines following subculture in liquid medium
(Instituto Oswaldo Cruz, Ministério da Saúde, 2000) Marie-France Bosseno; Nina Yacsik; Fernando Regla Vargas; Simone Frédérique Brénière
Previous studies showed that two groups of Trypanosoma cruzi clonal genotypes named clonet 20 and clonet 39 were predominant in Triatoma infestans, the unique vector of Chagas disease in Bolivia. These groups of clones correspond to distinct genetic clusters. These clonets were detected in T. infestans and Rhodnius pictipes fecal samples before isolation and after culture by kDNA PCR (polymerase chain reaction) and hybridization of the amplified products with clonet specific kDNA probes named 20 and 39 as previously reported. Forty eight T. infestans and three R. pictipes infected insects captured at random in different Bolivian departments were proceeded. As previously reported the direct identification of the two major clonets in fecal samples allowed the detection of abundant mixed infections: 41% in the original sample, however after culture, only 6% of mixed infections were detected. Among the 21 parasite stocks isolated from digestive tracts where mixed infections were initially detected (clonet 20 + 39) clonet 20 alone was detected in 81% of them. This result clearly showed that the culture step selected clonet 20 parasites over those belonging to clonet 39. The taxonomic status of the isolated stocks was also confirmed by isoenzyme typing, and correlation was observed between clustering topology and hybridization patterns with the probes 20 and 39.
Smallness of the Panmictic Unit of Triatoma infestans (Hemiptera: Reduviidae)
(Oxford University Press, 1998) Simone Frédérique Brénière; Marie-France Bosseno; Fernando Regla Vargas; Nina Yaksic; François Noireau; Sébastien Noël; Jean‐Pierre Dujardin; Michel Tibayrenc
The population genetic structure of Triatoma infestans (Klug), the principal vector of the causative agent of Chagas disease in Bolivia, was investigated by enzyme electrophoresis at 15 loci, of which 3 were polymorphic. A total of 1,286 adults and nymphs was collected from 19 localities of the Cochabamba (high endemicity) and La Paz (low endemicity) departments. Previous results were confirmed, including a low level of polymorphism (0.20), low genetic distance between geographic areas, and a population structure compatible with an isolation by distance model. However, a high proportion (26.3%) of the surveyed localities showed a significant excess of homozygotes, disputing previous conclusions that considered the village as the probable panmictic unit. The excess of homozygotes was reduced when smaller subunits, such as individual houses or chicken coops, were considered, indicating a Wahlund effect.
Sylvatic Triatomines (Hemiptera: Reduviidae) in Bolivia: Trends Toward Domesticity and Possible Infection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae)
(Oxford University Press, 1995) François Noireau; Marie-France Bosseno; Roxana Carrasco; Jenny Telleria; Fernando Regla Vargas; Clara Camacho; Nina Yaksic; Simone Frédérique Brénière
The risk of domestic transmission of Trypanosoma cruzi (Chagas) by sylvatic triatomines was assessed in an isolated area of the subandean region of Bolivia. None of the 390 residents examined had serological evidence of infection. Two sylvatic triatomine species, Eratyrus mucronatus (Stål) and Triatoma sordida (Stål), were found in houses and in peridomestic structures. The collection of nymphal instars of both species from some houses indicated possible domesticity. Microscopic examination of feces from 92 insects showed no parasites, and cultures from the guts of 30 insects were negative. Nevertheless, a polymerase chain reaction (PCR) test performed on the same fecal samples showed the presence of T. cruzi DNA in 19.1 and 12.5% of E. mucronatus and T. sordida, respectively. These 16 PCR-positive samples were hybridized with 2 T. cruzi-specific probes known from the domestic cycle in Bolivia (clones 20 and 39). At least 1 of these clones was identified in 7 bugs (5 E. mucronatus and 2 T. sordida). Moreover, no hybridization was observed with these probes in S E. mucronatus and 1 T. sordida samples that showed an amplified band by PCR. These data indicated that T. cruzi clones, genetically unrelated to clones 20 and 39, also were circulating in this area. Based on these results, the 2 sylvatic triatomine species encountered in Apolo should not be overlooked as possible local vectors of T. cruzi.
Trypanosoma cruzi discrete typing units (DTUs): Microsatellite loci and population genetics of DTUs TcV and TcI in Bolivia and Peru
(Elsevier BV, 2011) Christian Barnabé; Thierry De Meeûs; François Noireau; Marie-France Bosseno; Eric Marcelo Monje; François Renaud; Simone Frédérique Brénière
Trypanosoma cruzi genotypes associated with domestic Triatoma sordida in Bolivia
(Elsevier BV, 1998) Simone Frédérique Brénière; W. Morochi; Marie-France Bosseno; J.A. Ordóñez; Teresa de Jesús Molina Gutiérrez; Fernando Regla Vargas; Nina Yaksic; François Noireau
Trypanosoma cruzi:Study of the Distribution of Two Widespread Clonal Genotypes in BolivianTriatoma infestansVectors Shows a High Frequency of Mixed Infections
(Elsevier BV, 1996) Marie-France Bosseno; Jenny Telleria; Fernando Regla Vargas; Nina Yaksic; François Noireau; Annie Morin; Simone Frédérique Brénière
Wild Populations of Triatoma infestans Are Highly Connected to Intra-Peridomestic Conspecific Populations in the Bolivian Andes
(Public Library of Science, 2013) Simone Frédérique Brénière; Renata Salas; Rosio Buitrago; Philippe Brémond; Victor Sosa; Marie-France Bosseno; Etienne Waleckx; Stéphanie Depickère; Christian Barnabé
Triatoma infestans, the major vector of Chagas disease south of the Amazon in South America, has a large distribution of wild populations, contrary to what has previously been stated. These populations have been suspected of being the source of reinfestation of human habitats and could impede the full success of vector control campaigns. This study examined gene flow between intra-peridomestic populations and wild populations collected in the surround areas in three Andean localities in Bolivia. The populations were defined according to temporal, ecological, and spatial criteria. After DNA extraction from the legs of each insect, the samples were analyzed using seven microsatellite markers. First, the analysis of molecular variance (AMOVA) detected an absence of differentiation between wild and intra-peridomestic populations, although strong structuring was observed between the populations within each environment. Then for some populations, the Bayesian method of assignment to inferred populations showed very similar assignment patterns of the members of wild or intra-peridomestic populations in each locality. Finally, the detection of the first-generation migrants within the different populations provided evidence of insect displacement from the wild to the intra-peridomestic environment. This result indicates that, after control campaigns in the Andes, controlling this new paradigm of vector transmission risk stemming from the invasion of human habitats by wild populations of T. infestans requires long-term maintenance of public monitoring to keep the risk at a minimal level. Since wild populations of T. infestans have also been detected elsewhere in Argentina, Paraguay, and Chile, there is an urgent need to take these populations into account in future monitoring of Chagas disease transmission.