Browsing by Autor "Mayra Acosta-Suárez"

Now showing 1 - 3 of 3

Effect of heat treatment at high temperature on in vitro seeds germination of Phaseolus vulgaris cv. ‘ICA Pijao’
(2019) Dámaris Torres; Lourdes R. García; Novisel Veitía; Amanda Martirena Ramírez; Raúl Collado; Leonardo Rivero; Sinesio Torres; Mayra Acosta-Suárez
The common bean (Phaseolus vulgaris L.) production is limited by biotic and abiotic factors. Among them, water stress, saline or high temperatures. To solve this problem, genetic improvement programs are aimed at the search for tolerant cultivars. The objective of this work was to determine the effect of heat treatment at high temperatures on the in vitro germination of P. vulgaris cv. ‘ICA Pijao’. For this, seeds of 1-4 months of harvest were used. It were treated at 35 ± 2 °C in an incubator or immersed in water in a thermal bath. As a control, seeds at room temperature (28 ± 2 °C) were used. The results showed that in vitro germination was not less than 90%, however the two heat treatment conditions affected the length and thickness of the radicle and the length of the plumule. Considering the results, the ease of application and the non-affectation of germination, it is recommended to use the heat treatment at 35 ± 2 °C in an incubator. This could be used to induce high temperature stress in the common beans cv. ‘ICA Pijao’ in an in vitro selection protocol.
<i>In vitro</i> and <i>ex vitro</i> Selection of Potato Plantlets for Resistance to Early Blight
(Wiley, 2007) Novisel Veitía Rodríguez; Britta Kowalski; Lourdes R. García; Idalmis Bermúdez-Caraballoso; Mayra Acosta-Suárez; Pedro Orellana Pérez; Carlos Quintana; Nayanci Portal González; Reinaldo Quiñones Ramos
Abstract Reaction to the culture filtrate of Alternaria solani (Sorauer) was used as an indicator in an in vitro screening test to select lines with decreased field susceptibility to early blight from a population of 1000 putative mutants. Plantlets of cv. ‘Desirée derived from irradiated callus of potato were inoculated in vitro with a culture filtrate of A. solani (Sorauer). Of the 45 lines selected and subsequently evaluated under conditions of natural infection in the greenhouse six showed lesser degrees of early blight infection than the cv. Desirée control. The six lines selected in the greenhouse retained lower degrees of infection during 2 years of field trials.
Prevention of the bad dry of the malanga by treatment of natural, biological origin
(2014) Michel Chamizo Nicao; Daymí Isabel Carrazana García; Ernesto Espinosa Cuéllar; Annarella Chea González; Mayra Acosta-Suárez; René Cupull Santana
One of the most important causes of the decrease of yields in taro crops (Xanthosoma spp.) isthe roots rot caused by soil fungi (Bad dry) which it’s propagated by the agamic seed used as propagules. One of the most important measures to prevent it is the use of “in vitro” plants free of fungi. In this research was\nstudied the incorporation of several natural and biological products in the plots employed to acclimatize the “invitro” plants. Were evaluated: Trichoderma harzianum, Chitosan, and lobster shell (\nPanulirus argus (Latreille))and as control Mancozeb. The taro cultivars employed were Blanca INIVIT and Blanca Venegas, considerate as intermediated and susceptible respectively. Each plot was inoculated with propagules of Sclerotium rolsfii\n(Sacc.). In order to infer the action mechanism was conducted susceptible tests under “in vitro” conditions. The\nresults showed that the most susceptible cultivar was Blanca Venegas. All treatments decrease the incidence\nof dry rot, among these. T. harzianum\n produced the highest effect, antagonist capacity and antagonist effect type. The Chitosan and the lobster shell do not showed antifungal direct effect and probably act for stimulation\nof the protein synthesis related with the pathogenesis or structural barrier of defense in natural conditions.