Browsing by Autor "Michel Leiva-Mora"

Filter results by typing the first few letters
Now showing 1 - 4 of 4
  • Thumbnail Image
    Item type: Item ,
    Árbol filogenético y características de bacterias endófitas asociadas a Theobroma cacao L. en una zona de la provincia de Esmeraldas, Ecuador
    (Universidad Centroccidental Lisandro Alvarado, 2021) María Aracely Vera-Loor; Alexander Bernal-Cabrera; Danilo Vera; Michel Leiva-Mora; Alán Rivero-Aragón; Lisbeth Morales-Díaz de Villegas
    Dada la importancia que tendría el uso de bacterias endófitas para el control de las enfermedades del cacao, el objetivo de este estudio fue construir el árbol filogenético y caracterizar las bacterias endofíticas formadoras de endosporas en función de la altitud, la edad y el tejido de la planta de cacao en una zona productora de la provincia de Esmeraldas en Ecuador. El aislamiento de las bacterias se realizó a partir de secciones de tejidos de mazorcas y pedicelos de la flor en cultivares de cacao Criollo tipo Nacional. Se obtuvieron 45 aislados de bacterias endófitas formadoras de endosporas, asociados principalmente con tejidos del endocarpio. El árbol filogenético reveló que todas las bacterias pertenecen al género Bacillus. No se encontró relación entre el número de aislados bacterianos con la edad de la planta, aunque hubo una ligera tendencia a la disminución de los aislados hacia los sitios de mayor altitud.
  • Thumbnail Image
    Item type: Item ,
    Isolation and Characterization of Phosphate-Solubilizing Rhizobacteria from Solanum tuberosum with Plant Growth-Promoting Activity
    (2026) Michel Leiva-Mora; Pamela Elizabeth Mera Guzmán; Rafael Isaías Mera Andrade; Alicia Monserrath Zabala Haro; Luis Rodrigo Saa; Paúl Loján; Catherine Lizzeth Silva Agurto; Luis Fabián Salazar-Garcés; Betty Beatriz González Osorio; Dariel Cabrera Mederos
    Phosphate-solubilizing rhizobacteria associated with the Solanum tuberosum L. cultivar ‘Superchola’ were isolated and characterized to improve our understanding of plant growth promotion in agricultural systems. Bacteria were isolated by serial dilutions, and the morphology of the colonies was characterized on nutrient agar culture medium. In addition, morphological identification was achieved by Gram staining. The ability to solubilize phosphate was assessed in Pikovskaya agar culture medium, while molecular identification involved the amplification of the partial 16S rRNA gene using the polymerase chain reaction. In the Píllaro canton, the highest number of colony-forming units per gram of soil was recorded at 9.72 × 109. Among the isolated strains, 62% exhibited circular morphology, 92% had a smooth texture, and 85% displayed entire margins. Notably, 83% of the isolates were Gram-negative, with 50% exhibiting a bacillary form. The most effective phosphate solubilizers were from the Mocha canton, particularly the isolate CC-FCAGP-BSF6, which showed superior solubilization capacity. Molecular identification revealed bacterial isolates from four genera, i.e., Bacillus, Pseudomonas, Lysinibacillus, and Paenibacillus. These strains exhibited significant phosphate solubilization in vitro and resulted in increased leaf area (0.21–0.49, p = 0.038), fresh mass (0.46–0.87, p = 0.014), dry mass (0.092–0.096, p = 0.047), and leaf area index (0.14–0.33, p = 0.026) in the S. tuberosum cultivar ‘Superchola’ in vitro plants. This study identifies bacterial species associated with the rhizosphere of S. tuberosum in Ecuador and highlights their potential for promoting plant growth and solubilizing phosphates.
  • Thumbnail Image
    Item type: Item ,
    Somatic embryogenesis and plant regeneration from leaf of the interspecific hybrid of mahogany (Swietenia macrophylla King × S. mahagoni (L.) Jacq.)
    (Springer Science+Business Media, 2021) Elisa Quiala; Raúl Barbón; Saúl Mestanza; Mariana La O; Gardenis Merlan; Jenny Elizabeth Núñez-Ramos; Naivy Pérez; Michel Leiva-Mora; Elio Jiménez; Dion Daniels
  • Thumbnail Image
    Item type: Item ,
    The first report on the occurrence race 9 of the tomato leaf mold pathogen Cladosporium fulvum (syn. Passalora fulva) in Cuba
    (Springer Science+Business Media, 2021) Alexander Bernal-Cabrera; Benedicto Martínez-Coca; Lidcay Herrera-Isla; Danay Ynfante Martínez; Belkis Peteira-Delgado; Orelvis Portal; Michel Leiva-Mora; P.J.G.M. de Wit