Browsing by Autor "Mikel R. Stevens"

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A genetic linkage map of quinoa (Chenopodium quinoa) based on AFLP, RAPD, and SSR markers
(Springer Science+Business Media, 2004) Peter J. Maughan; Alejandro Bonifacio; Eric N. Jellen; Mikel R. Stevens; Craig E. Coleman; M.L. Ricks; Sara Mason; David E. Jarvis; Brian Gardunia; Daniel J. Fairbanks
Assessment of genetic diversity in the USDA and CIP-FAO international nursery collections of quinoa (<i>Chenopodium quinoa</i> Willd.) using microsatellite markers
(Cambridge University Press, 2007) Shawn A. Christensen; Donald B. Pratt; Charles S. Pratt; Paul Nelson; Mikel R. Stevens; Eric N. Jellen; Craig E. Coleman; Daniel J. Fairbanks; Alejandro Bonifacio; Peter J. Maughan
Quinoa ( Chenopodium quinoa Willd.) is a staple food crop for millions of impoverished rural inhabitants of Andean South America where it has been cultivated for millennia. Interest in quinoa, due largely to its superior nutritional characteristics, is fuelling a growing export market and has led to an increased focus on genetic research and the development of quinoa breeding programmes throughout South America. The success of these breeding programmes will rely heavily on the development of core germplasm collections and germplasm conservation. We report the development of a set of fluorescence-tagged microsatellite molecular markers that can be used to characterize genetic diversity within quinoa germplasm and we use this set of 36 microsatellites markers to genetically characterize the diversity of 121 accessions of C. quinoa held in the USDA germplasm bank, 22 accessions from the CIP-FAO international nursery collection and eight accessions representing parents from genetic mapping populations. A total of 420 alleles were detected among the quinoa accessions with an average of 11 alleles detected per microsatellite locus. Genetic heterogeneity was observed in 32% of the quinoa accessions at a given locus and suggests that many of these accessions represent heterogeneous seed lots or landraces. Both unweighted pair-group method with arithmetic averages (UPGMA) and principle components analysis (PCA) analyses partitioned the quinoa accessions into two main clusters. The first major cluster consisted of accessions from the Andean highlands of Peru, Bolivia, Ecuador, Argentina and extreme northeastern Chile. The other main cluster contained accessions from both the lowlands of Chile and a set of USDA accessions with no known passport data, collected by Emigdio Ballón. Using the patterns of genetic diversity detected within the C. quinoa accessions we discuss hypotheses regarding quinoa's centre of diversity, including highland and lowland ecotype clustering patterns, origin of lowland varieties, origin of domestication, and diversity levels in the USDA and CIP-FAO collections.
Construction of a quinoa (Chenopodium quinoa Willd.) BAC library and its use in identifying genes encoding seed storage proteins
(Springer Science+Business Media, 2006) Mikel R. Stevens; Craig E. Coleman; Susan E. Parkinson; Peter J. Maughan; H.-B. Zhang; Marie Balzotti; David L. Kooyman; K. Arumuganathan; Alejandro Bonifacio; Daniel J. Fairbanks
Development and use of an expressed sequenced tag library in quinoa (Chenopodium quinoa Willd.) for the discovery of single nucleotide polymorphisms
(Elsevier BV, 2004) Nathan D. Coles; Craig E. Coleman; Shawn A. Christensen; Eric N. Jellen; Mikel R. Stevens; Alejandro Bonifacio; J. Rojas‐Beltrán; Daniel J. Fairbanks; Peter J. Maughan
Development and use of microsatellite markers for genetic diversity analysis of cañahua (Chenopodium pallidicaule Aellen)
(Springer Science+Business Media, 2010) A. Vargas; D. B. Elzinga; J. Rojas‐Beltrán; Alejandro Bonifacio; Brad Geary; Mikel R. Stevens; Eric N. Jellen; Peter J. Maughan
Development and Use of Microsatellite Markers for Germplasm Characterization in Quinoa (<i>Chenopodium quinoa</i> Willd.)
(Wiley, 2005) Sara Mason; Mikel R. Stevens; Eric N. Jellen; Alejandro Bonifacio; Daniel J. Fairbanks; Craig E. Coleman; Ronald McCarty; Andrew Rasmussen; Peter J. Maughan
Quinoa ( Chenopodium quinoa Willd.) is a widely consumed food crop and a primary protein source for many of the indigenous inhabitants of the Andean region of South America. The objective of this study was to develop a collection of reproducible and highly informative microsatellite markers for quinoa. A total of 1276 clones were sequenced from three microsatellite‐enriched (CA, ATT, ATG) libraries. Four hundred fifty‐seven (36%) of the clones contained unique microsatellites. The most common repeated motifs, other than CA, AAT, and ATG, were GA and CAA. Flanking primers were designed for 397 microsatellite loci and screened using a panel of diverse quinoa accessions and one accession of C. berlandieri Moq., a wild relative of quinoa. Two hundred eight (52%) of the microsatellite markers were polymorphic among the quinoa accessions. An additional 25 of the microsatellite markers (6%) were polymorphic when the C. berlandieri accession was included in the analysis. Only in rare instances (nine) did a microsatellite amplify in quinoa and not in C. berlandieri The number of observed alleles ranged from 2 to 13, with an average of four alleles detected per locus. Heterozygosity values ranged from 0.20 to 0.90 with a mean value of 0.57. Sixty‐seven markers (32%) were highly polymorphic ( H ≥ 0.70). These microsatellites markers are an ideal resource for use in managing quinoa germplasm, trait mapping and marker‐assisted breeding strategies. The wide cross‐species transportability of these markers may extend their value to research involving other Chenopodium species.
Quinoa (Chenopodium quinoa)
(2007) Peter J. Maughan; Alejandro Bonifacio; Craig E. Coleman; Eric N. Jellen; Mikel R. Stevens; Daniel J. Fairbanks
Simple sequence repeat marker development and genetic mapping in quinoa (Chenopodium quinoa Willd.)
(Springer Nature, 2008) David E. Jarvis; Olga R. Kopp; Eric N. Jellen; Melanie A. Mallory; Jack Pattee; Alejandro Bonifacio; Craig E. Coleman; Mikel R. Stevens; Daniel J. Fairbanks; Peter J. Maughan