Browsing by Autor "Nasser, Julio R"

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Multilocus sequence typing approach for a broader range of species of Leishmania genus: describing parasite diversity in Argentina.
(2015) Marco, Jorge D; Barroso, Paola A; Locatelli, Fabricio M; Cajal, S Pamela; Hoyos, Carlos L; Nevot, M Cecilia; Lauthier, Juan J; Tomasini, Nicolás; Juarez, Marisa; Estévez, J Octavio; Korenaga, Masataka; Nasser, Julio R; Hashiguchi, Yoshihisa; Ruybal, Paula
Leishmaniasis is a vector-borne protozoan infection affecting over 350 million people around the world. In Argentina cutaneous leishmaniasis is endemic in nine provinces and visceral leishmaniasis is spreading from autochthonous transmission foci in seven provinces. However, there is limited information about the diversity of the parasite in this country. Implementation of molecular strategies for parasite typing, particularly multilocus sequence typing (MLST), represents an improved approach for genetic variability and population dynamics analyses. We selected six loci as candidates implemented in reference strains and Argentinean isolates. Phylogenetic analysis showed high correlation with taxonomic classification of the parasite. Autochthonous Leishmania (Viannia) braziliensis showed higher genetic diversity than L. (Leishmania) infantum but low support was obtained for intra-L. braziliensis complex variants suggesting the need of new loci that contribute to phylogenetic resolution for an improved MLST or nested-MLST scheme. This study represents the first characterization of genetic variability of Leishmania spp. in Argentina.
Tegumentary leishmaniasis and sand flies in a border area between Argentina and Bolivia.
(2019) Copa, Griselda N; Almazán, María C; Aramayo, Lorena V; Krolewiecki, Alejandro J; Cajal, Silvana P; Juarez, Marisa; Lauthier, Juan J; Korenaga, Masataka; Barroso, Paola; Nasser, Julio R; Marco, Jorge D; Gil, José F
BACKGROUND: Some sand flies are of medical importance because they are vectors of Leishmania parasites that are responsible for leishmaniasis. The aim of this study was to make a retrospective epidemiological analysis of tegumentary leishmaniasis (TL), to identify Leishmania spp. from patient isolates and to describe the diversity of sand flies from a border area between Bolivia and Argentina. METHODS: TL cases included in the study were diagnosed in an endemic area of the north of Argentina from 1985 to 2017. The parasites isolated were characterized by the cytochrome B method. Sand flies were captured with Centers for Disease Control traps in Aguas Blancas and Media Luna-Algarrobito localities. RESULTS: A total of 118 cases of TL were analysed. Eight isolates were characterized as Leishmania (Viannia) braziliensis. A total of 1291 sand flies were captured, including Nyssomyia neivai, Cortelezzii complex, Evandromyia sallesi, Migonemyia migonei and Micropygomyia quinquefer. Within the area, sand flies were found in the backyards of houses. CONCLUSIONS: In this region there exists the possibility of peridomestic transmission of TL in the neighbourhoods peripheral to the urban area and in rural environments as well as the risk of transmission to travellers that pass through the customs offices.
Vegetation Cover and Microspatial Distribution of Sand Flies (Diptera: Psychodidae) in an Endemic Locality for Cutaneous Leishmaniasis in Northern Argentina.
(2018) Chanampa, Mariana Del M; Gleiser, Raquel M; Hoyos, Carlos L; Copa, Griselda N; Mangudo, Carolina; Nasser, Julio R; Gil, José F
The sand fly fauna in Hipólito Yrigoyen, Argentina, a locality where cutaneous leishmaniasis cases occur, was surveyed with zones of higher abundance of sand flies correlated to vegetation cover estimated through normalized difference vegetation index (NDVI). Sand flies were collected with 10 CDC traps during six nights, from December 2009 to January 2010. A map was built of expected sand flies abundance in which levels of NDVI were categorized. In total, 1,392 Phlebotominae (Diptera: Psychodidae) specimens were collected, comprised of the following species: Nyssomyia neivai (Pinto 1926), Migonemyia migonei (França 1920), species of the cortelezzii complex (Brèthes 1923), Evandromyia sallesi (Galvão & Coutinho 1940), and Psathyromyia shannoni (Dyar 1929). Positive correlations were found between the abundance of sand flies and the NDVI (P < 0.05) for buffer areas of <150 m radii from the trap location points, i.e., the sand fly abundance was greater where vegetation cover and density were greater. In this context, plant cover should be taken into account to prioritize surveillance and control areas within the program of sand flies control in northern Argentina.