Browsing by Autor "Nataniel Mamani"

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Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli
(Frontiers Media, 2022) Jessica Guzman-Otazo; Enrique Joffré; Jorge Agramont; Nataniel Mamani; Jekaterina Jutkina; Fredrik Boulund; Yue Hu; Daphne Jumilla-Lorenz; Anne Farewell; D. G. Joakim Larsson
Watersheds contaminated with municipal, hospital, and agricultural residues are recognized as reservoirs for bacteria carrying antibiotic resistance genes (ARGs). The objective of this study was to determine the potential of environmental bacterial communities from the highly contaminated La Paz River basin in Bolivia to transfer ARGs to an Escherichia coli lab strain used as the recipient. Additionally, we tested ZnSO4 and CuSO4 at sub-inhibitory concentrations as stressors and analyzed transfer frequencies (TFs), diversity, richness, and acquired resistance profiles. The bacterial communities were collected from surface water in an urban site close to a hospital and near an agricultural area. High transfer potentials of a large set of resistance factors to E. coli were observed at both sites. Whole-genome sequencing revealed that putative plasmids belonging to the incompatibility group N (IncN, IncN2, and IncN3) were predominant among the transconjugants. All IncN variants were verified to be mobile by a second conjugation step. The plasmid backbones were similar to other IncN plasmids isolated worldwide and carried a wide range of ARGs extensively corroborated by phenotypic resistance patterns. Interestingly, all transconjugants also acquired the class 1 integron intl1, which is commonly known as a proxy for anthropogenic pollution. The addition of ZnSO4 and CuSO4 at sub-inhibitory concentrations did not affect the transfer rate. Metal resistance genes were absent from most transconjugants, suggesting a minor role, if any, of metals in the spread of multidrug-resistant plasmids at the investigated sites.
Cost-effectiveness of rotavirus vaccination in Bolivia from the state perspective
(Elsevier BV, 2011) Emily R. Smith; Emily Rowlinson; Volga Iñiguez; Kizee A. Etienne; Rosario Rivera; Nataniel Mamani; Richard Rheingans; Maritza Patzi; Percy Halkyer; Juan S. León
Enfermedades Diarreicas Agudas asociadas a Rotavirus
(Elsevier BV, 2007) C R Romero; Nataniel Mamani; Kjetil Halvorsen; Volga Iñiguez
In the period of 2001-2002, a total of 1163 fecal samples were collected from children less than five years of age with diarrhea, at the Hospital Albina Patio and other health centers in Cochabamba city. Rotavirus infection general prevalence was 19% (220), distributed among hospitalized children 24% (77) and outpatients 17% (143). Rotavirus infections were observed along the whole study period, with highest prevalence in coldest months: April (24%), May (34%) and June (28%) and lowest frequencies (8-15%) in February, August, and September. Major rotavirus outbreak was significantly associated with the driest and colder season. The highest percentage of rotavirus infections (36,3%) was found among children between 7-12 months of age. Moderate dehydration, vomits and fever were the clinical symptoms more frequently associated with rotavirus acute gastroenteritis, being dehydration more common in hospitalized patients. The distribution of P and G genotypes analyzed by RT-PCR was: G1 (44%), G2 (6%), P[8] (24%) and P4 (15%). A co-infection P[8]P[6] was observed in 3 samples (4%). Genotype P[6] was found associated to outpatients. These findings, highlight that rotavirus is an important cause of acute gastroenteritis and that any of the currently available vaccines would potentially be protective against circulating strains found in the city of Cochabamba.
Impact of urban contamination of the La Paz River basin on thermotolerant coliform density and occurrence of multiple antibiotic resistant enteric pathogens in river water, irrigated soil and fresh vegetables
(Springer International Publishing, 2016) Violeta Poma; Nataniel Mamani; Volga Iñiguez
Rotavirus associated acute gastroenteritis
(2005) C R Romero; Nataniel Mamani; Kjetil Halvorsen; Volga Iñiguez
Rotavirus genotype distribution during the pre-vaccine period in Bolivia: 2007–2008
(Elsevier BV, 2013) Rosario Rivera; Kristen Forney; Maria René Castro; Paulina A. Rebolledo; Nataniel Mamani; Maritza Patzi; Percy Halkyer; Juan S. León; Volga Iñiguez