Browsing by Autor "Ninoska Flores"

Now showing 1 - 20 of 23

An unprecedented chlorine-containing piperamide from Piper pseudoarboreum as potential leishmanicidal agent
(Elsevier BV, 2019) Ninoska Flores; Juan C. Ticona; Pablo Bilbao-Ramos; María Auxiliadora Dea‐Ayuela; Juan Celidonio Ruiz Macedo; Isabel L. Bazzocchi; F. Bolás‐Fernández; Ignacio A. Jiménez
Antiparasitic activity of prenylated benzoic acid derivatives from Piper species
(Elsevier BV, 2009) Ninoska Flores; Ignacio A. Jiménez; Alberto Giménez; Grace Ruiz; David Gutiérrez; Geneviève Bourdy; Isabel L. Bazzocchi
Antiparasitic Metabolites from Hyptis brevipes, a Tacana Medicinal Plant
(SAGE Publishing, 2019) Ivan Limachi; Claudia Condo; Camila Palma; Nélida Nina; Efraín Salamanca; Juan C. Ticona; Enrique Udaeta; Ninoska Flores; Alcides Serato; Natalio Marupa
A bioassay screening against protozoa parasites of several Tacana medicinal plants gave Hyptis brevipes (Id'ene eidhue), traditionally used as decoction for intestinal parasites, as the most active extract. In this work we did a bioguided isolation of active constituents found in leaves. Structure elucidation was carried out by NMR spectroscopy and MS spectrometry analyses. Active constituents showed differentiated activity towards Giardia lamblia, Trypanosoma cruzi, several Leishmania strains, Plasmodium falciparum and cytotoxicity against HeLa cells. Brevipolide H (1) was the less cytotoxic and best antiparasitic, while the catechol derivative (2) the most active and cytotoxic.
Benzoic Acid Derivatives from Piper Species and Their Antiparasitic Activity
(American Chemical Society, 2008) Ninoska Flores; Ignacio A. Jiménez; Alberto Giménez; Grace Ruiz; David Gutiérrez; Geneviève Bourdy; Isabel L. Bazzocchi
Piper glabratum and P. acutifolium were analyzed for their content of main secondary constituents, affording nine new benzoic acid derivatives (1, 2, 4, 5, 7, and 10-13), in addition to four known compounds (3, 6, 8, and 9). Their structures were elucidated on the basis of spectroscopic data. Riguera ester reactions and optical rotation measurements established the new compounds as racemates. In the search for antiparasitic agents, the compounds were evaluated in vitro against the promastigote forms of Leishmania spp., Trypanosoma cruzi, and Plasmodium falciparum. Among the evaluated compounds, methyl 3,4-dihydroxy-5-(3'-methyl-2'-butenyl)benzoate (7) exhibited leishmanicidal effect (IC50 13.8-18.5 microg/mL) against the three Leishmania strains used, and methyl 3,4-dihydroxy-5-(2-hydroxy-3-methylbutenyl)benzoate (1), methyl 4-hydroxy-3-(2-hydroxy-3-methyl-3-butenyl)benzoate (3), and methyl 3,4-dihydroxy-5-(3-methyl-2-butenyl) benzoate (7) showed significant trypanocidal activity, with IC50 values of 16.4, 15.6, and 18.5 microg/mL, respectively.
Conocimientos locales, prácticas y usos contemporáneos atribuidos a la ayahuasca en el noroeste de la Amazonia peruana
(Sociedad Argentina de Botánica, 2025) E. Hanz Rodríguez-Cabrera; M. Alejandra Romero van-Heurck; Ani T. Cruz-Garcia; Ana N. Sandoval Vergara; Ninoska Flores; Bruno A. Angulo-Pinedo; Piero M. Araujo-Chávez; Astriht Ruíz Ríos; Elsa L. Rengifo-Salgado; Darío Pérez
Introducción y objetivos: La ayahuasca es una bebida tradicionalmente empleada con fines curativos, tanto físicos como espirituales. Su uso se ha popularizado globalmente, generando interés turístico y una creciente demanda. Esta investigación tuvo como objetivo documentar y analizar los conocimientos locales, las prácticas asociadas a la toma y los usos contemporáneos de la ayahuasca en el noroeste de la Amazonia peruana, mediante un enfoque de métodos mixtos. M&M: El trabajo de campo se desarrolló en mercados y centros naturistas, donde se aplicaron entrevistas semiestructuradas a informantes y chamanes locales. Los datos se analizaron mediante estadística descriptiva e inferencial (χ² y prueba exacta de Fisher), complementados con indicadores de frecuencia de citación para etnovariedades y usos terapéuticos de la ayahuasca. Resultados: La mayoría de los informantes y los chamanes conocen sobre la ayahuasca, atribuyéndole usos como purgante espiritual, digestivo y terapéutico, aunque reconocen efectos secundarios. Ambos grupos señalaron su empleo en el tratamiento de brujería, enfermedades psicológicas y digestivas, bajo prácticas rituales dirigidas por chamanes. Se identificaron 26 etnovariedades, siendo cielo ayahuasca la más conocida y utilizada. Conclusiones: El uso de la ayahuasca persiste gracias al conocimiento local y su adaptación a las dinámicas del mercado, donde los chamanes son quienes mayoritariamente han conservado la memoria sobre su preparación y diversidad, ajustando los rituales a una demanda progresiva. Así, la práctica chamanística se convierte en una experiencia terapéutica accesible a un público más amplio, haciendo que su uso no sea exclusivamente local.
(E)-Piplartine Isolated from Piper pseudoarboreum, a Lead Compound against Leishmaniasis
(Multidisciplinary Digital Publishing Institute, 2020) Juan C. Ticona; Pablo Bilbao-Ramos; Ninoska Flores; María Auxiliadora Dea‐Ayuela; F. Bolás‐Fernández; Ignacio A. Jiménez; Isabel L. Bazzocchi
The current therapies of leishmaniasis, the second most widespread neglected tropical disease, have limited effectiveness and toxic side effects. In this regard, natural products play an important role in overcoming the current need for new leishmanicidal agents. The present study reports a bioassay-guided fractionation of the ethanolic extract of leaves of Piper pseudoarboreum against four species of Leishmania spp. promastigote forms, which afforded six known alkamides (1-6). Their structures were established on the basis of spectroscopic and spectrometric analysis. Compounds 2 and 3 were identified as the most promising ones, displaying higher potency against Leishmania spp. promastigotes (IC50 values ranging from 1.6 to 3.8 µM) and amastigotes of L. amazonensis (IC50 values ranging from 8.2 to 9.1 µM) than the reference drug, miltefosine. The efficacy of (E)-piplartine (3) against L. amazonensis infection in an in vivo model for cutaneous leishmaniasis was evidenced by a significant reduction of the lesion size footpad and spleen parasite burden, similar to those of glucantime used as the reference drug. This study reinforces the therapeutic potential of (E)-piplartine as a promising lead compound against neglected infectious diseases caused by Leishmania parasites.
Estandarizacion de un metodo fluorometrico sobre trofozoitos de giardia lamblia
(2015) Claudia Condo Montero; Efraín Salamanca; Ninoska Flores; Alberto Giménez Turba
Ethnopharmacology of Wild Plants
(2020) Amner Muñoz-Acevedo; María Carmen González; Alma Polo-Barrios; Martha Cervantes Díaz; Ricardo D.D.G. de Alburquerque; Ninoska Flores; Alberto Giménez-Turba; María Elena Cazar; Chabaco Armijos; Omar Malagón-Avilés
The traditional herbal healers, along with wild medicinal plants and the knowledge they have about them, in certain regions of Latin America, are the prevalent alternatives for some people/communities to cover any primary health care requirements (e.g., treatment to prevent/improve/release pain/symptoms/disorders/diseases). traditional (plant-based) medicine has gained worldwide acceptance because the plants used for this purpose have been apparently efficacious and safe for a long time (decades/centuries),\nthese being some reasons why the WHO created the traditional, complementary and integrative unit. (WHO 2019).\nDespite this, it is important to remember (i) what “ethnopharmacology” studies are about, which are, shortly, the uses, possible action modes, and bioproperties of plant-based preparations; and (ii) the contribution made by phytochemistry and pharmacology along with other related sciences (botany, pharmacy, microbiology, virology, parasitology, etc.) to find/check any biologically active drugs (molecules/fractions/extracts). In that sense, traditional medicine systems need more evidence-based studies that allow establishing the pharmacological potential together with the validation of therapeutic uses of medicinal plants and its chemical constituents [isolated or mixtures (fractions/extracts)] in such a way that safety and effectiveness are verified, as well as to reveal/understand their preventive and/or therapeutic mechanisms (Mukherjee et al. 2010, Schmidt and Klaser-Cheng 2017).
Evaluation of substituted phenalenone analogues as antiplasmodial agents
(Elsevier BV, 2013) David Gutiérrez; Ninoska Flores; Teresa Abad-Grillo; Grant McNaughton‐Smith
Flavonoids from Piper delineatum modulate quorum-sensing-regulated phenotypes in Vibrio harveyi
(Elsevier BV, 2015) Alberto J. Martín‐Rodríguez; Juan C. Ticona; Ignacio A. Jiménez; Ninoska Flores; José J. Fernández; Isabel L. Bazzocchi
Flavonoids from Piper Species as Promising Antiprotozoal Agents against Giardia intestinalis: Structure-Activity Relationship and Drug-Likeness Studies
(Multidisciplinary Digital Publishing Institute, 2022) Juan C. Ticona; Pablo Bilbao-Ramos; Ángel Amesty; Ninoska Flores; María Auxiliadora Dea‐Ayuela; Isabel L. Bazzocchi; Ignacio A. Jiménez
Diarrhea diseases caused by the intestinal protozoan parasite Giardia intestinalis are a major global health burden. Moreover, there is an ongoing need for novel anti-Giardia drugs due to drawbacks with currently available treatments. This paper reports on the isolation and structural elucidation of six new flavonoids (1-6), along with twenty-three known ones (7-29) from the Piper species. Their structures were established by spectroscopic and spectrometric techniques. Flavonoids were tested for in vitro antiprotozoal activity against Giardia intestinalis trophozoites. In addition, structure-activity relationship (SAR) and in silico ADME studies were performed to understand the pharmacophore and pharmacokinetic properties of these natural compounds. Eight flavonoids from this series exhibited remarkable activity in the micromolar range. Moreover, compound 4 was identified as having a 40-fold greater antiparasitic effect (IC50 61.0 nM) than the clinical reference drug, metronidazole (IC50 2.5 µM). This antiprotozoal potency was coupled with an excellent selectivity index (SI 233) on murine macrophages and in silico drug-likeness. SAR studies revealed that the substitution patterns, type of functional group, and flavonoid skeleton played an essential role in the activity. These findings highlight flavonoid 4 as a promising candidate to develop new drugs for the treatment of Giardia infections.
IN VITRO PRODUCTION OF LEISHMANICIDAL ALKALOID 2 PHENYL-QUINOLINE BY ANGOSTURA LONGIFLORA KRAUSE A
(Universidad Autónoma del Estado de México, 2008) Marina Guarachi; Magaly Paz; Rogelio Chuqui; Michel Sauvain; Ninoska Flores; Ma Teresa Alvarez; Alberto Giménez
"Angostura longiflora (K.) Kallunki (Rutaceae) is a medicinal wood tree used by Amazonian tribes for the treatment of stomach parasites and leishmaniasis. Cell cultures from leaves of this species were obtained on Murashige and Skoog (MS) medium supplemented with kinetine (kin) and 2,4-dichlorophenoxyacetic acid (2,4-D) based on a factorial design, where concentrations of sucrose and phosphate were changed. The increasing of sucrose and phosphate enhanced the specific growth rate from 0.16 to 0.33d-1. The leishmanicidal alkaloid, 2-phenyl quinoline was isolated from calli and identified by means of nuclear magnetic resonance (NMR) and mass (EI) spectra. This is the first report concerning the production, isolation and chemical characterization by NMR and mass spectra of the main alkaloid, in Evanta leaves, 2-phenyl-quinoline from cell cultures of A. longiflora."
Laccase mediator system for activation of agarose gel: Application for immobilization of proteins
(Elsevier BV, 2010) Laura Mendoza; Gashaw Mamo; Ninoska Flores; Alberto Giménez; Rajni Hatti‐Kaul
Leishmaniasis in the major endemic region of Plurinational State of Bolivia: Species identification, phylogeography and drug susceptibility implications
(Elsevier BV, 2017) Pablo Bilbao-Ramos; María Auxiliadora Dea‐Ayuela; Oscar Victor Cardenas-Alegría; Efraín Salamanca; José Antonio Santalla-Vargas; C. Benito; Ninoska Flores; F. Bolás‐Fernández
Leishmanicidal Constituents from the Leaves of Piper rusbyi
(Thieme Medical Publishers (Germany), 2007) Ninoska Flores; Gabriela M. Cabrera; Ignacio A. Jiménez; José E. Piñero; Alberto Giménez; Geneviève Bourdy; Fernando Cortés-Selva; Isabel L. Bazzocchi
The kavapyrone (+)-(7 R,8 S)-epoxy-5,6-didehydrokavain (1) and the chalcone flavokavain B (2) were isolated from Piper rusbyi as the bioactive components by bioassay-guided fractionation, using an in vitro assay against promastigote forms of three Leishmania strains. In addition, the new kavapyrone, (7 R,8 R/7 S,8 S)-dihydroxy-5,6-didehydrokavain (3), which is very likely an artifact, and four known compounds (4-7) were isolated. Their structures were elucidated on the basis of spectral analysis, and the absolute configurations of compounds 1 and 3 were established by CD studies and the modified Mosher ester procedure, respectively. All compounds were evaluated for in vitro leishmanicidal activity. The most active compounds 1 (IC50=81.9 microM) and 2 (IC50=11.2 microM) were also evaluated in vivo against a New World strain of cutaneous leishmaniasis, and the results showed the efficacy of 2 at a dose of 5 mg/kg/day. Compounds 1 and 3 were also assayed as reversal agents against a multidrug-resistant Leishmania tropica line, but were found to be inactive.
Mulinane-type diterpenoids from Azorella compacta display antiplasmodial activity
(Elsevier BV, 2004) Luis Loyola; Jorge Bórquez; Glauco Morales; Aurelio San-Martı́n; José Dárias; Ninoska Flores; Alberto Giménez
Preparation and antimalarial activity of semisynthetic lycorenine derivatives
(Elsevier BV, 2013) Juan Carlos Cedrón; David Gutiérrez; Ninoska Flores; Ángel G. Ravelo; Ana Estévez‐Braun
Promising Antimicrobial Agents from Some Latin American Medicinal Plants Against Disease-Causing Pathogens in Humans and Animals
(2022) Amner Muñoz-Acevedo; María C. González; Osnaider J. Castillo; Cindy P. Guzmán; Sandra Rodríguez-Acosta; Martha Cervantes Díaz; Ricardo D.D.G. de Alburquerque; Bettina Monika Ruppelt; Ninoska Flores; Alberto Giménez-Turba
Susceptibilidad in vitro de promastigotes de Leishmania frente a Anfotericina B, Miltefosina y los Alcaloides Totales de Galipea longiflora mediante el Método Colorimétrico XTT-PMS
(2015) Efraín Salamanca; Danitza Xiomara Romero; José A Santalla; P Oporto; Ninoska Flores; Alberto Giménez Turba
Synthesis and antimalarial activity of new haemanthamine-type derivatives
(Elsevier BV, 2012) Juan Carlos Cedrón; David Gutiérrez; Ninoska Flores; Ángel G. Ravelo; Ana Estévez‐Braun