Browsing by Autor "Omar Tenorio"

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Biología reproductiva de dos poblaciones de Triatoma infestans (Hemiptera: Reduviidae) en condiciones de laboratorio
(Universidad Internacional de La Rioja, 2011) Jorge Espinoza; Marinely Bustamante; Ana Lineth García; Omar Tenorio; François Noireau; Demetrio Rivera; Mirko Rojas Cortez
Objectives: in the present study, we documented the reproductive biology of two populations of T. infestans, from the mesothermic\nAndean Valleys (20 de Octubre) and the Chaco Serrano (Mataral) in laboratory conditions. Methods: there was used 15 pairs of T. infestans\npopulations (Mataral and 20 de Octubre). The control population was colonies of T. infestans from laboratory. The insects were fed on\nchickens for 45 minutes and kept in a climate chamber at 28 ± 3 ° C, 60 ± 5%. The following parameters and rates were calculated: Fecundity,\nFertility, oviposition index (IO), fertility index (FI). Results: it was observed that the T. infestans strain from 20 de Octubre locality,\npresented the greatest number of eggs per day, with a daily fecundity average rate of (1.95 ± 1.5), a fertility of 95.6% (IO) averaged 0.43\n(IF) average of 0.9. Statistical analysis shown that the 20 de octubre locality, presented significant values between variables of number\nof eggs and the weight of triatomines with a ratio of R2 = 0.77 and a significant Pearson correlation. Conclusions: under laboratory\nconditions two different populations of T. infestans coming from different regions of Bolivia, shown different patterns of fecundity and\nfertility, these data are of valuable importance to be considered by vector control programs, since showed that the life cycle of the same\nvector species may vary according to geographical area, therefore, is very important to continue with studies that describe and help to\nunderstand the biology of the main vector species in Bolivia.
Tripanosomátidos aislados de mamíferos silvestres en tres departamentos de Bolivia (Cochabamba, Potosí y Santa Cruz de la Sierra)
(Universidad Internacional de La Rioja, 2013) Mary Cruz Torrico; Tatiana Tellez; Omar Tenorio; Laura J. Rojas; J.C. Huaranca; Anabelle de la Barra; Ana Lineth García; Faustino Torrico
Objectives: The aim of this research was isolate kinetoplastid protozoan from wild mammals in three departments of Bolivia, to identify\nTrypanosomatids reservoirs that could cause infection in different reservoirs and disease in humans. Methods: The wild mammals were\ncaught in the Chaco, valleys and the tropical zone of Bolivia, using Sherman, Havahard and Tomahawk traps. Captured animals were\nanesthetized and xenodiagnosis and blood cardiac puncture was performed; trypanosomatides isolation using blood culture was done\nin NNN culture media and the respective identification was performed by PCR-RFLP techniques in the molecular biology laboratory of\nIIBISMED. Results: 236 wild mammals belonging to 30 species were captured, of which 7 species showed infection by hemoflagellates.\nTrypanosoma cruzi was isolated from Didelphis marsupialis, D. albiventris, Galea musteloides, Graomys domorum and Andalgalomis pearsoni;\nT.c. marinkellei and T. dionisii were isolated from Carolia perspicillata (bats) and other kinetoplastid not identified by available molecular\ntools were also isolated from Andalgalomys and Graomys mammals genus, from Campero and Cordillera provinces of Cochabamba and\nSanta Cruz. Conclusions: The T. cruzi, T.c. marinkellei, T. dionisii and other trypanosomatids are infecting marsupials (Didelphis), rodents\n(Graomys and Andalgalomys) and wild guinea pigs (Galea) which are found in a sylvatic cycle in the studied areas.