Browsing by Autor "Pablo Iturri"

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Complex II‐linked mitochondrial respiration is upregulated during postnatal development in high‐altitude grown mice
(Wiley, 2022) Christian Arias‐Reyes; Fernanda Aliaga‐Raduán; Oscar M. Rollano‐Peñaloza; Pablo Iturri; Jorge Soliz; Vincent Joseph
Recently, we have shown that chronic normobaric hypoxia (12% O 2 – 21 days) triggers mitochondrial and cell‐ metabolic plasticity in the retrosplenial cortex of male adult FVB mice but not in SD rats. In these animals we reported a transient upregulation of anaerobic metabolic pathways (glycolysis + lactate metabolism) followed by an attenuation in the mitochondrial respiration. These results pointed to an optimized use of oxygen at cellular level in an area of the brain responsible of processing visual information and spatial memory. Features of great importance in the biological fitness. In the current work we investigate how the residence in high altitude (La Paz – Bolivia, 3,600 m) affects the developmental profile of the mitochondrial respiratory efficiency in the retrosplenial cortex of mice and rats. We hypothesize that the mitochondrial plasticity observed in adult mice will have a process of maturation during postnatal development. This response shall be absent in rats. To do so, we measured the mitochondrial oxygen consumption rate (OCR) in saponin‐permeabilized brain samples from FVB mice and SD rats born and grown in La Paz. The animals were sacrificed at postnatal ages p7, p14, p21, and adulthood (P90), and the OCR linked to the use of NADH (N pathway), FADH 2 (S pathway), or both (NS pathway) was quantified by means of high‐resolution respirometry (OROBOROS). Our results show a pattern of maturation (increase) of the mitochondrial respiration in the retrosplenial cortex along with the postnatal development (21 days+) of mice and rats. However, an upregulation of the participation of the S pathway (complex II‐linked) in the mitochondrial respiration was observed during the first three weeks of age only in mice. This observation supports previous works suggesting a key role of the complex II and succinate (substrate of complex II) in the successful cellular and mitochondrial acclimatization to hypoxia. These findings contribute to further establish FVB mice and SD rats as a model to study divergent acclimatization to hypoxia.
Efficient breathing at neonatal ages: A sex and Epo-dependent issue
(Elsevier BV, 2016) Pablo Iturri; Aïda Bairam; Jorge Soliz
Erythropoietin and caffeine exert similar protective impact against neonatal intermittent hypoxia: Apnea of prematurity and sex dimorphism
(Elsevier BV, 2019) Sofien Laouafa; Pablo Iturri; Christian Arias‐Reyes; François Marcouiller; Marcelino Gonzales; Vincent Joseph; Aïda Bairam; Jorge Soliz
Inflammation severity, rather than respiratory failure, is strongly associated with mortality of ARDS patients in high-altitude ICUs
(Frontiers Media, 2025) Daniel Molano-Franco; Joan Ramón Masclans Enviz; Antonio Viruez‐Soto; Mario Gómez; Hugo Rojas; Edgar Beltrán; Víctor Manuel García Nieto; Fernanda Aliaga‐Raduán; Pablo Iturri; Christian Arias‐Reyes
The study concludes that, in high-altitude settings, ARDS patient survival in ICUs is more closely associated with managing inflammatory responses than with focusing solely on respiratory parameters. Further large-scale studies are recommended to validate the impact of inflammatory marker monitoring on survival outcomes in high-altitude ICUs.
Inhibition of Protein Kinases AKT and ERK1/2 Reduce the Carotid Body Chemoreceptor Response to Hypoxia in Adult Rats
(Springer Nature, 2015) Pablo Iturri; Vincent Joseph; Gloria Rodrigo; Aïda Bairam; Jorge Soliz
Plasticity of mitochondrial proton leak in adults and newborn mice as a possible strategy for high altitude adaptation
(Elsevier BV, 2024) Maud Demarest; Fernanda Aliagia-Raduan; Pablo Iturri; Christian Arias‐Reyes; Edith M. Schneider Gasser; Jorge Soliz; Vincent Joseph