Browsing by Autor "R. Colque"

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Improved Production of Galanthamine and Related Alkaloids by Methyl Jasmonate inNarcissus confususShoot-Clumps
(Thieme Medical Publishers (Germany), 2004) R. Colque; Françesc Viladomat; Jaume Bastida; Carles Codina
Liquid-shake cultured shoot-clumps of Narcisus confussus were treated with the commonly used biotic elicitors methyl jasmonate, arachidonic acid, chitosan and salicylic acid. The effects of these compounds on the growth of the explants, as well as on the amount of the alkaloids released to the liquid culture medium and accumulated in the tissues at the end of the experiment were studied. The obtained results showed that, in general, high doses of these compounds had a negative effect on the growth of the explants, particularly the salicylic acid. On the contrary, the addition of methyl jasmonate, mainly at 25 microM, promoted the release of galanthamine and other related alkaloids to the liquid medium in proportions of up to 300% in relation to the control explants, and also their accumulation in tissues. The other elicitors studied did not have any interesting effects on the production of these Amaryllidaceae-type alkaloids.
In vitro production of bulblets ofCyrtanthus loddigesianusandCyrtanthus speciosus
(Taylor & Francis, 2003) Mario Angulo; R. Colque; Françesc Viladomat; Jaume Bastida; Carles Codina
SummaryThe micropropagation of Cyrtanthus loddigesianus and C. speciosus was carried out by the twin-scaling technique. In both species the best induction of shoots was obtained on the Murashige and Skoog (MS) medium containing 5.37 μM of a-naphthaleneacetic acid (NAA) and 22 20 μM of benzyladenine (BA), whereas the best regeneration rate was found in the medium with 0.5% of activated charcoal. High doses of NAA/BA (5.37 μM/8.88 μM) were also found to be the most effective for developing new shoots in both species. 90 g l–1 and 30 g l–1 of sucrose in the liquid medium appeared to be the most appropriate condition to increase the biomass of the shoot-clumps of C. loddigesianus and C. speciosus, respectively. Rooting was promoted by low NAA concentrations (1.34 or 2.68 μM) in C. loddigesianus bulblets, and high concentrations (5.37 μM) in C. speciosus bulblets. Before transferring the obtained plantlets to the ex vitro conditions some were cold-treated to break possible dormancy. The plantlets were acclimatized in different chambers, and finally transferred to soil, where they showed 100% survival.
Mass propagation of Cyrtanthus clavatus and Cyrtanthus spiralis using liquid medium culture
(Elsevier BV, 2003) G.P Morán; R. Colque; Françesc Viladomat; Jaume Bastida; Carles Codina
Micropropagation of the rareEucrosia stricklandii(Amaryllidaceae) by twin-scaling and shake liquid culture
(Taylor & Francis, 2002) R. Colque; Françesc Viladomat; Jaume Bastida; Carles Codina
SummaryBulbs of E. stricklandii were introduced into in vitro culture by the twin-scaling technique. Different Murashige and Skoog (MS) media supplemented with 0.5% w/v activated charcoal, naphthaleneacetic acid (NAA)/benzyladenine (BA) 0.54/4.44 μM or NAA/Thidiazuron (TDZ) 0.54/0.45 μM were used for shoot induction. Media with different combinations of 2,4-dichlorphenoxyacetic acid (2,4-D) and BA were tested during the secondary multiplication. The shoots obtained in the multiplication step were then transferred to a liquid medium with different sucrose concentrations (from 0.088 to 0.351 M) to increase biomass. Shoot bulbing was induced in 0.263.M sucrose semi-solid medium without growth regulators. Several media containing different concentrations of NAA (ranging from 0 to 5.37 μM) combined with various sucrose concentrations (from 0.044 to 0.263 M) were also tested for root induction and the bulblets were finally transplanted to soil. The best induction of shoots was obtained in the media with activated charcoal or with TDZ, but those treated with activated charcoal showed a better regeneration rate and the shoots were better formed. The best development of new shoots was obtained in the medium containing 0.45 and 4.44 μM of 2,4-D and BA, respectively. 0.175–0.263 M sucrose in the liquid medium appeared to be the most appropriate condition for increase in biomass. Rooting was promoted by low NAA concentrations (0.537–1.343 μM), and 0.175 M sucrose was the best treatment for root development.