Browsing by Autor "Renata Salas"

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Combination of cytochrome b heteroduplex-assay and sequencing for identification of triatomine blood meals
(Elsevier BV, 2011) Rosio Buitrago; Stéphanie Depickère; Marie-France Bosseno; Edda Siñani Patzi; Etienne Waleckx; Renata Salas; Claudia Aliaga; Simone Frédérique Brénière
Eco-epidemiologia de la enfermedad de Chagas en comunidades pobres de Bolivia
(European Organization for Nuclear Research, 2012) Frédéric Lardeux; Lizet Zambrana; Claudia Aliaga; Renata Salas; Gino Torrico; T. Chavez
First Report of Widespread Wild Populations of Triatoma infestans (Reduviidae, Triatominae) in the Valleys of La Paz, Bolivia
(American Society of Tropical Medicine and Hygiene, 2010) Rosio Buitrago; Pablo Vidaurre; Faustine Zovéda; Simone Frédérique Brénière; Etienne Waleckx; Renata Salas; Marie-France Bosseno; François Noireau; Elio Mamani
Wild populations of Triatoma infestans, the main vector of Chagas disease in the Southern Cone countries, may be involved in reinfestation of human dwellings, limiting the success of vector-control campaigns in Bolivia. Knowledge of the distribution of these populations remains incomplete. We report here the detection of T. infestans wild populations in large areas in the department of La Paz, Bolivia. Among 18 sylvatic areas investigated, 17 were positive with T. infestans specimens. The infection rate of captured T. infestans with Trypanosoma cruzi was 85.7% in adult specimens. These results expand the geographical distribution of wild populations of T. infestans; it may be distributed throughout the Inter-Andean Dry Forest eco-region of Bolivia. The current information allows us to propose the hypothesis that a sylvatic origin of the reinfestation is located in the valleys of La Paz.
Genetic Characterization of Trypanosoma cruzi DTUs in Wild Triatoma infestans from Bolivia: Predominance of TcI
(Public Library of Science, 2012) Simone Frédérique Brénière; Claudia Aliaga; Etienne Waleckx; Rosio Buitrago; Renata Salas; Christian Barnabé; Michel Tibayrenc; François Noireau
By exploring large-scale DTUs that infect the wild populations of T. infestans, this study opens the discussion on the origin of TcI and TcV DTUs that are predominant in domestic Bolivian cycles.
New Discoveries of Sylvatic Triatoma infestans (Hemiptera: Reduviidae) Throughout the Bolivian Chaco
(American Society of Tropical Medicine and Hygiene, 2012) Etienne Waleckx; Stéphanie Depickère; Renata Salas; Claudia Aliaga; Marcelo Monje; Hiber Calle; Rosio Buitrago; François Noireau; Simone Frédérique Brénière
Sylvatic populations of Triatoma infestans might be involved in the recolonization of human dwellings. We report here the discoveries of new T. infestans sylvatic foci in the Bolivian Chaco. Eighty-one triatomines were caught, 38 of which were identified as T. infestans. Triatoma sordida and Panstrongylus geniculatus were the other species collected. One T. infestans and one T. sordida were infected with Trypanosoma cruzi TcI; one T. infestans was infected with TcII. These discoveries add to the debate on the geographic distribution of sylvatic T. infestans populations, the geographic origin of the species, and the epidemiological role of these populations.
New insights on the Chagas disease main vector Triatoma infestans (Reduviidae, Triatominae) brought by the genetic analysis of Bolivian sylvatic populations
(Elsevier BV, 2011) Etienne Waleckx; Renata Salas; Nerida Huamán; Rosio Buitrago; Marie-France Bosseno; Claudia Aliaga; Christian Barnabé; Roberto Rodríguez; Faustine Zovéda; Marcelo Monje
Wild Populations of Triatoma infestans Are Highly Connected to Intra-Peridomestic Conspecific Populations in the Bolivian Andes
(Public Library of Science, 2013) Simone Frédérique Brénière; Renata Salas; Rosio Buitrago; Philippe Brémond; Victor Sosa; Marie-France Bosseno; Etienne Waleckx; Stéphanie Depickère; Christian Barnabé
Triatoma infestans, the major vector of Chagas disease south of the Amazon in South America, has a large distribution of wild populations, contrary to what has previously been stated. These populations have been suspected of being the source of reinfestation of human habitats and could impede the full success of vector control campaigns. This study examined gene flow between intra-peridomestic populations and wild populations collected in the surround areas in three Andean localities in Bolivia. The populations were defined according to temporal, ecological, and spatial criteria. After DNA extraction from the legs of each insect, the samples were analyzed using seven microsatellite markers. First, the analysis of molecular variance (AMOVA) detected an absence of differentiation between wild and intra-peridomestic populations, although strong structuring was observed between the populations within each environment. Then for some populations, the Bayesian method of assignment to inferred populations showed very similar assignment patterns of the members of wild or intra-peridomestic populations in each locality. Finally, the detection of the first-generation migrants within the different populations provided evidence of insect displacement from the wild to the intra-peridomestic environment. This result indicates that, after control campaigns in the Andes, controlling this new paradigm of vector transmission risk stemming from the invasion of human habitats by wild populations of T. infestans requires long-term maintenance of public monitoring to keep the risk at a minimal level. Since wild populations of T. infestans have also been detected elsewhere in Argentina, Paraguay, and Chile, there is an urgent need to take these populations into account in future monitoring of Chagas disease transmission.