Browsing by Autor "Richard J. Roberts"

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    Genome Sequence and Methylation Pattern of Haloterrigena salifodinae BOL5-1, an Extremely Halophilic Archaeon from a Bolivian Salt Mine
    (American Society for Microbiology, 2021) Priya DasSarma; Brian P. Anton; Satyajit L. DasSarma; Hedvig A. L. von Ehrenheim; Fabiana Lilian Martínez; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
    The halophilic archaeon <i>Haloterrigena salifodinae</i> BOL5-1 was isolated from a Bolivian salt mine and sequenced using single-molecule real-time sequencing. The GC-rich genome was 5.1 Mbp, with a 4.2-Mbp chromosome and 5 plasmids ranging from 96 to 281 kbp. The genome annotation was incorporated into HaloWeb (https://halo.umbc.edu), and the methylation patterns were incorporated into REBASE (http://tools.neb.com/genomes/view.php?seq_id=99167&list=1).
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    Genome Sequence and Methylation Patterns of <i>Halorubrum</i> sp. Strain BOL3-1, the First Haloarchaeon Isolated and Cultured from Salar de Uyuni, Bolivia
    (American Society for Microbiology, 2019) Priya DasSarma; Brian P. Anton; Satyajit L. DasSarma; Victoria J. Laye; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
    <i>Halorubrum</i> sp. strain BOL3-1 was isolated from Salar de Uyuni, Bolivia, and sequenced using single-molecule real-time sequencing. Its 3.7-Mbp genome was analyzed for gene content and methylation patterns and incorporated into the Haloarchaeal Genomes Database (http://halo.umbc.edu). The polyextremophilic character and high-elevation environment make the microbe of interest for astrobiology.
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    Genome Sequence of <i>Halobacterium</i> sp. Strain BOL4-2, Isolated and Cultured from Salar de Uyuni, Bolivia
    (American Society for Microbiology, 2021) Priya DasSarma; Brian P. Anton; Hedvig A. L. von Ehrenheim; Fabiana Lilian Martínez; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
    <i>Halobacterium</i> sp. strain BOL4-2 was isolated from an Andean salt flat, Salar de Uyuni, in Bolivia. Single-molecule real-time (SMRT) sequencing revealed a 2.4-Mbp genome with a 2.0-Mbp chromosome and four plasmids (2 to 299 kb). Its isolation from an environment experiencing multiple extremes makes the strain interesting for astrobiology.
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    Genome Sequences and Methylation Patterns of Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, Two Extremely Halophilic Archaea from a Bolivian Salt Mine
    (American Society for Microbiology, 2019) Priya DasSarma; Brian P. Anton; Satyajit L. DasSarma; Fabiana Lilian Martínez; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
    Two extremely halophilic archaea, namely, <i>Natrinema versiforme</i> BOL5-4 and <i>Natrinema pallidum</i> BOL6-1, were isolated from a Bolivian salt mine and their genomes sequenced using single-molecule real-time sequencing. The GC-rich genomes of BOL5-4 and BOL6-1 were 4.6 and 3.8 Mbp, respectively, with large chromosomes and multiple megaplasmids. Genome annotation was incorporated into HaloWeb and methylation patterns incorporated into REBASE.