Browsing by Autor "S. P. Quigley"

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Effect of the concentration of Spirulina (Spirulina platensis) algae in the drinking water on water intake by cattle and the proportion of algae bypassing the rumen
(CSIRO Publishing, 2010) Tanda Panjaitan; S. P. Quigley; S. R. McLennan; D. P. Poppi
Spirulina, a freshwater microalgae, has previously been shown to increase the efficiency of microbial protein production in cattle fed hay with a low crude protein content. The present study was carried out to determine the effect of increasing the concentration of Spirulina in the drinking water on the intake of water and the amount of water containing Spirulina bypassing the rumen of cattle. Five rumen-cannulated steers were given a fixed amount of pangola grass hay (14 g DM/kg W.day–1) and water containing 0, 1, 2, 2.7 and 3.5% (w/w) Spirulina in an incomplete Latin square design. Water intake by the control steers (0% Spirulina) was 29.7 and 49.3 g/kg W for the first drinking event after it was made available and over 24 h, respectively. For steers receiving the algae, intake of water plus Spirulina increased linearly (P &lt; 0.01) from 42.7 to 60.2 g/kg W during the first drinking event, as the concentration of Spirulina in the drinking water increased, but over 24 h was not affected by Spirulina concentration and averaged 74.4 g/kg W. The bypass of water through the rumen, as determined using chromium-EDTA as a marker, averaged 20.5 ± 1.2% and was not affected by the concentration of Spirulina in the drinking water. Increasing inclusion of Spirulina was associated with a decrease in rumen pH, an increase in urea concentration in blood serum, and an increase in ammonia-N concentration, propionate and branched-chain fatty acids, and a decrease in butyrate proportions in rumen fluid. Spirulina inclusion in the drinking water increased water intake and may provide a potential safe and inexpensive alternative to urea for extensively grazed ruminants.
Rice straw, cassava by-products and tree legumes provide enough energy and nitrogen for liveweight maintenance of Brahman (Bos indicus) cows in Indonesia
(CSIRO Publishing, 2014) Risa Antari; G. P. Ningrum; Dianne Mayberry; Marsetyo Marsetyo; Dicky Pamungkas; S. P. Quigley; D. P. Poppi
The aim of this experiment was to test the effectiveness of two diets in increasing liveweight (LW) and body condition score (BCS) of Brahman cows in Indonesia. Diets were based on rice straw, with additional energy and nitrogen (N) provided in the form of onggok (a cassava by-product) plus urea or a tree legume. Thirty mature, non-pregnant, non-lactating Brahman crossbred cows (288 kg LW, BCS 2/5) were allocated to one of two treatment groups. Cows were kept in individual pens for 21 weeks and offered one of two diets; (1) urea-supplemented rice straw ad libitum plus 10 g onggok DM/kg LW.day, or (2) untreated rice straw ad libitum plus 5 g onggok DM/kg LW.day and 5 g Gliricidia sepium DM/kg LW.day. The urea supplement provided no advantages over using locally available N sources such as gliricidia, with both supplement types meeting the rumen-degradable N requirements of the cows. Cows on both diets gained weight at a similar rate (0.19 kg/day) for the first 15 weeks of the experiment. Liveweight gain in Weeks 16–21 was only 0.04 kg/day, despite an increase in total feed intake and energy content of the diets compared with Weeks 1–15. Liveweight of cows stabilised during Weeks 16–21 at 304 kg, BCS 2.2. While our results demonstrate that Brahman cows can maintain LW on rice straw-based diets, they may not be able to maintain a BCS sufficient for good reproduction rates (i.e. BCS 3 or higher on 1–5 scale). Better quality diets containing higher levels of energy are required for cows to gain and maintain a suitable BCS.
Spirulina (Spirulina platensis) algae supplementation increases microbial protein production and feed intake and decreases retention time of digesta in the rumen of cattle
(CSIRO Publishing, 2014) Tanda Panjaitan; S. P. Quigley; S. R. McLennan; A. J. Swain; D. P. Poppi
Cattle consuming pastures low in protein have low liveweight gain due to low rumen degradable protein (RDP) supply and thus low microbial crude protein (MCP) production and efficiency of MCP production [EMCP, g MCP/kg digestible organic matter (DOM)]. Nitrogen supplements can increase MCP production and EMCP of cattle grazing low protein pastures. The objective of this study was to compare the effects of supplementation with a non-protein-N source (NPN), in this case urea and ammonium sulfate (US), with a single-cell algal protein source (Spirulina platensis), on intake, microbial protein supply and digestibility in cattle. Nine cannulated Bos indicus steers [initial liveweight 250.1 ± 10.86 (s.d.) kg] were fed Mitchell grass hay (Astrebla spp; 6.1 g N, 746 g NDF/kg DM) ad libitum and were supplied with increasing amounts of US (0, 6, 13, 19 and 33 g US DM/kg hay DM) or Spirulina 0, 0.5, 1.4, 2.5 and 6.1 g Spirulina DM/kg W.day in an incomplete Latin square design. The response of MCP production and EMCP to increasing amounts of the two supplements was different, with a greater response to Spirulina evident. The MCP production was predicted to peak at 140 and 568 g MCP/day (0.64 and 2.02 g MCP/kg W.day) for the US and Spirulina supplements, respectively. The highest measured EMCP were 92 and 166 g MCP/kg DOM for the US and Spirulina treatments at 170 and 290 g RDP/kg DOM, respectively, or a Spirulina intake of 5.7 g DM/kg W.day. Increasing RDP intake from US and Spirulina resulted in an increase in Mitchell grass hay intake and rumen NH3-N concentration and reduced the retention time of liquid and particulate markers and digesta DM, NDF and lignin in the rumen with greater changes due to Spirulina. Total DM intake peaked at a Spirulina supplement level of 4.6 g Spirulina DM/kg W.day with a 2.3-fold higher DOM intake than Control steers. Rumen NH3-N concentrations reached 128 and 264 mg NH3-N/L for the US and Spirulina treatments with a significant increase in the concentration of branched-chain fatty acids for the Spirulina treatment. The minimum retention time of liquid (Cr-EDTA; 23 and 13 h) and particulate (Yb; 34 and 22 h) markers in the rumen were significantly lower for Spirulina compared with US and lower than unsupplemented animals at 24 and 34 h for Cr-EDTA and Yb, respectively. Spirulina could be provided safely at much higher N intakes than NPN supplements. The results suggest that, at an equivalent RDP supply, Spirulina provided greater increases than US in MCP production, EMCP and feed intake of Bos indicus cattle consuming low protein forage and could also be fed safely at higher levels of N intake.