Browsing by Autor "Sandra Milena Leal Pinto"

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    Antifungal activity of Co(II) and Cu(II) complexes containing 1,3-bis(benzotriazol-1-yl)-propan-2-ol on the growth, virulence traits of fluconazole-resistant Candida: Synthesis, DFT calculations and biological activity
    (Research Square (United States), 2022) Ricardo A. Murcia-Galán; Sandra M. Durán; Sandra Milena Leal Pinto; Martha V. Roa; José D. Vargas; Laura V. Herrera; Alvaro Muñoz‐Castro; Desmond MacLeod‐Carey; Tonny W. Naranjo; John Hurtado
    Abstract Relevant virulence traits in Candida are associated with the dimorphic change and biofilm formation, which became an important target to reduce the antifungal resistance. In this work, Co(II) complexes containing a benzotriazole derivative ligand showed a promising capacity of reduce these virulence traits. These complexes exhibited higher antifungal activities than the free ligands against all the Candida albicans and non- albicans strains tested, where compounds 2 and 4 showed minimum inhibitory concentration values between 15.62 and 125 µg mL − 1 . Moreover, four complexes ( 2 – 5 ) of Co(II) and Cu(II) with benzotriazole ligand were synthesized. These compounds were obtained as air-stable solids and characterized by melting point, thermogravimetric analysis, infrared, Raman and ultraviolet/visible spectroscopy. The analysis of the characterization data allowed to identify that all the complexes had 1:1 (M:L) stoichiometries. Additionally, Density Functional Theory calculations were carried out for 2 and 3 to propose a probable geometry of both compounds. The conformer Da of 2 was the most stable conformer according to the Energy Decomposition Analysis; while the conformers of 3 have a fluxional behavior in this analysis that didn’t allow to recognize the most probable conformer. These results provide an important platform for the design of new compounds with antifungal activities and capacity of attack other target of relevance to reduce the antimicrobial resistance.
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    Chemical composition and antiprotozoal activities of Colombian Lippia spp essential oils and their major components
    (Instituto Oswaldo Cruz, Ministério da Saúde, 2010) Patricia Escobar; Sandra Milena Leal Pinto; Laura Viviana Herrera; Jairo René Martı́nez; Elena E. Stashenko
    The chemical composition and biological activities of 19 essential oils and seven of their major components were tested against free and intracellular forms of Leishmania chagasi and Trypanosoma cruzi parasites as well as Vero and THP-1 mammalian cell lines. The essential oils were obtained from different species of Lippia, a widely distributed genus of Colombian plants. They were extracted by microwave radiation-assisted hydro-distillation and characterised by GC-FID and GC-MS. The major components were geranial, neral, limonene, nerol, carvacrol, p-cymene, gamma-terpinene, carvone and thymol. The essential oil of Lippia alba exhibited the highest activity against T. cruzi epimastigotes and intracellular amastigotes with an IC50 of 5.5 microg/mL and 12.2 microg/mL, respectively. The essential oil of Lippia origanoides had an IC50 of 4.4 microg/mL in L. chagasi promastigotes and exhibited no toxicity in mammalian cells. Thymol (IC50 3.2 +/- 0.4 microg/mL) and S-carvone (IC50 6.1 +/- 2.2 microg/mL), two of the major components of the active essential oils, were active on intracellular amastigotes of T. cruziinfected Vero cells, with a selective index greater than 10. None of the essential oils or major components tested in this study was active on amastigotes of L. chagasi infected THP-1 cells.