Browsing by Autor "Shiladitya DasSarma"

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16S rRNA Gene Diversity in Ancient Gray and Pink Salt from San Simón Salt Mines in Tarija, Bolivia
(American Society for Microbiology, 2020) Wolf T. Pecher; Fabiana Lilian Martínez; Priya DasSarma; Daniel Guzmán; Shiladitya DasSarma
DNA from 250 million-year-old pink and gray salts from mines in Tarija, Bolivia, subjected to 16S rRNA gene amplicon sequencing and analysis provided evidence for similar but distinct prokaryotic communities. The results constitute a snapshot of archaeal and bacterial microorganisms in these remote and ancient salt deposits.
16S rRNA Gene Diversity in the Salt Crust of Salar de Uyuni, Bolivia, the World’s Largest Salt Flat
(American Society for Microbiology, 2020) Wolf T. Pecher; Fabiana Lilian Martínez; Priya DasSarma; Daniel Guzmán; Shiladitya DasSarma
Salar de Uyuni is a vast, high-altitude salt flat in Bolivia with extreme physico-geochemical properties approaching multiple limits of life. Evidence for diverse halophilic bacteria and archaea was found in its surface and near-surface salt crust using 16S amplicon analysis, providing a snapshot of prokaryotic life.
Genome Sequence and Methylation Pattern of Haloterrigena salifodinae BOL5-1, an Extremely Halophilic Archaeon from a Bolivian Salt Mine
(American Society for Microbiology, 2021) Priya DasSarma; Brian P. Anton; Satyajit L. DasSarma; Hedvig A. L. von Ehrenheim; Fabiana Lilian Martínez; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
The halophilic archaeon Haloterrigena salifodinae BOL5-1 was isolated from a Bolivian salt mine and sequenced using single-molecule real-time sequencing. The GC-rich genome was 5.1 Mbp, with a 4.2-Mbp chromosome and 5 plasmids ranging from 96 to 281 kbp. The genome annotation was incorporated into HaloWeb (https://halo.umbc.edu), and the methylation patterns were incorporated into REBASE (http://tools.neb.com/genomes/view.php?seq_id=99167&list=1).
Genome Sequence and Methylation Patterns of Halorubrum sp. Strain BOL3-1, the First Haloarchaeon Isolated and Cultured from Salar de Uyuni, Bolivia
(American Society for Microbiology, 2019) Priya DasSarma; Brian P. Anton; Satyajit L. DasSarma; Victoria J. Laye; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
Halorubrum sp. strain BOL3-1 was isolated from Salar de Uyuni, Bolivia, and sequenced using single-molecule real-time sequencing. Its 3.7-Mbp genome was analyzed for gene content and methylation patterns and incorporated into the Haloarchaeal Genomes Database (http://halo.umbc.edu). The polyextremophilic character and high-elevation environment make the microbe of interest for astrobiology.
Genome Sequence of Halobacterium sp. Strain BOL4-2, Isolated and Cultured from Salar de Uyuni, Bolivia
(American Society for Microbiology, 2021) Priya DasSarma; Brian P. Anton; Hedvig A. L. von Ehrenheim; Fabiana Lilian Martínez; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
Halobacterium sp. strain BOL4-2 was isolated from an Andean salt flat, Salar de Uyuni, in Bolivia. Single-molecule real-time (SMRT) sequencing revealed a 2.4-Mbp genome with a 2.0-Mbp chromosome and four plasmids (2 to 299 kb). Its isolation from an environment experiencing multiple extremes makes the strain interesting for astrobiology.
Genome Sequences and Methylation Patterns of Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, Two Extremely Halophilic Archaea from a Bolivian Salt Mine
(American Society for Microbiology, 2019) Priya DasSarma; Brian P. Anton; Satyajit L. DasSarma; Fabiana Lilian Martínez; Daniel Guzmán; Richard J. Roberts; Shiladitya DasSarma
Two extremely halophilic archaea, namely, Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, were isolated from a Bolivian salt mine and their genomes sequenced using single-molecule real-time sequencing. The GC-rich genomes of BOL5-4 and BOL6-1 were 4.6 and 3.8 Mbp, respectively, with large chromosomes and multiple megaplasmids. Genome annotation was incorporated into HaloWeb and methylation patterns incorporated into REBASE.
Genomic Analysis of Haloarchaea from Diverse Environments, including Permian Halite, Reveals Diversity of Ultraviolet Radiation Survival and DNA Photolyase Gene Variants
(Multidisciplinary Digital Publishing Institute, 2023) Sagorika Nag; Priya DasSarma; David J. Crowley; Rafael Hamawi; Samantha Tepper; Brian P. Anton; Daniel Guzmán; Shiladitya DasSarma
Ultraviolet (UV) radiation responses of extremophilic and archaeal microorganisms are of interest from evolutionary, physiological, and astrobiological perspectives. Previous studies determined that the halophilic archaeon, Halobacterium sp. NRC-1, which survives in multiple extremes, is highly tolerant of UV radiation. Here, Halobacterium sp. NRC-1 UV tolerance was compared to taxonomically diverse Haloarchaea isolated from high-elevation salt flats, surface warm and cold hypersaline lakes, and subsurface Permian halite deposits. Haloterrigena/Natrinema spp. from subsurface halite deposits were the least tolerant after exposure to photoreactivating light. This finding was attributed to deviation of amino acid residues in key positions in the DNA photolyase enzyme or to the complete absence of the photolyase gene. Several Halobacterium, Halorubrum and Salarchaeum species from surface environments exposed to high solar irradiance were found to be the most UV tolerant, and Halorubrum lacusprofundi from lake sediment was of intermediate character. These results indicate that high UV tolerance is not a uniform character trait of Haloarchaea and is likely reflective of UV exposure experienced in their environment. This is the first report correlating natural UV tolerance to photolyase gene functionality among Haloarchaea and provides insights into their survival in ancient halite deposits and potentially on the surface of Mars.