Browsing by Autor "Sima Nasri"

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Antimicrobial Effect of Zataria Essential Oil on the Skin Bacteria in Wistar Rats
(2020) Soheila Faramarz Isfahanian; Maryam Sadrnia; Sima Nasri; Hamid Sobhanian
Objective: Zataria is one of the native plants of Iran which is widely used for the treatment of diseases among Iranians. In this study, we investigated the antimicrobial effects of Zataria essential oil on the skin bacteria in rats. Methods: Bacterial strains were isolated from the skin of 6 wistar rats and the antimicrobial effects of Zataria essential oil were evaluated by disk diffusion and microbroth dilution methods. In-vivo tests were performed to evaluate the antimicrobial effect of the essential oil by microbial culture as well as allergy tests on the skin of experimental rats compared to controls. Results: Three bacterial strains were isolated from the skin of rats identified as Staphylococcus aureus, Corynebacterium and Staphylococcus epidermidis. Minimum Growth Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for the two strains of Staphylococcus aureus and Corynebacterium were obtained 0.39 and 0.78 mg/ml, while for Staphylococcus epidermidis, they were 0.195 and 0.39 mg/ml, respectively. In-vivo test results showed the antibacterial effect of the essential oil on the skin bacteria and no inflammatory effects were observed under the allergy test. Conclusion: Zataria essential oil has antimicrobial effects on the skin infections in lower concentrations. The use of this essential oil as an antiseptic and preservative in cosmetics is recommended instead of chemical preservatives that generally have skin side effects.
Antinociceptive effect of thymoquinone in diabetic rats and the role of serum lipid peroxidation
(2012) Poordahandeh Uranus; Sima Nasri; Mehrdad Roghani; Parvin Salehi; Tourandokht Baluchnejadmojarad
Effect of Kiwifruit Extract (Actinidia deliciosa) on Body Weight, Thyroid Hormones, Pain and Memory in Hypothyroidism Male Rats
(2015) Khadijeh Hasoonizadeh Fallahi; Sima Nasri; Mohammad Kazem Gharib-Naseri; Alireza Sarkaki; Shahram Mashhadizadeh
Background and Objectives: Oxidative stress due to hypothyroidism causes behavioral deficits. Kiwifruit contains various vitamins and antioxidants. In this study the effect of kiwifruit extract on body weight, memory, pain and serum T4 and T3 in hypothyroidism male rats was evaluated. Subjects and Methods: Wistar male rats(200-250g)were dividedinto 8 groups (n=8) randomely as following: 1) Control, the remaining groups were hypothyroidism that received methimazole (500mg/L in drinking water) for 3 weeks. 2-7) Treated groups received orally either different doses of Kiwifruit extract (50,100,150, 200, 400 mg/kg/5ml or the same volume of normal saline for 14 days. 8) Positive control group receiving 1 mg/L of levothyroxine in drinking water. Serum thyroid hormones (with radioimmunoassay), body weight, pain (by tail-flick test), and memory (by shutle box) were measured for all groups. Data were analyzed with one-way ANOVA followed by Tukey’s post hoc and PResults: Serum T4 and T3, body weight and memory and pain reflex latency were decreased significantly after induction of hypothyroidism (P<0.01). Treatment with 150 and 200 mg/kg doses, without affecting the thyroid hormone serum level, improved memory and pain significantly (P<0.001) Higher doses of extract had no effect on above parameters. Conclusion: Treatment with kiwifruit extract didn’t change thyroid hormones; therefore, had no effect on hypothalamus-pituitary-thyroid axis. Improving effects of Kiwifruit extract on behavioral deficits following hypothyroidism might be related to its antioxidant property in brain.
Effect of Selenium on the motility, morphology and viability of sperm cells after freezing and thawing procedure
(2014) Sima Nasri; Fardin Amidi; Zahra Sadat Rezaeian
iAbstract Background: Selenium is essential for the normal function of sperm cells and spermatogenesis. This element as an antioxidant cofactor reduces oxygen free radicals, and is expected to be effective for increasing fertility. Objective: The aim of this study was to determine the effect of selenium on motility, morphology and viability of sperm cells after freezing and thawing procedure. Methods: This experimental study was performed on semen samples from 42 males with normal semen parameters referred to Shariati infertility center in Tehran during February and March 2013. Each sample was divided into four equal parts (two parts were washed and the other two parts were remained unwashed). 5 µg/l selenium was added to one of the washed and one of the unwashed sperm parts. Two other parts were remained untreated as control groups. Every four parts were freezed for two weeks, and subsequently they were thawed and motility, morphology, and viability of sperm cells were assessed. Data were analyzed by one-way ANOVA. Findings: After defreezing, motility, morphology, and viability of sperm cells in selenium groups were significantly different from control groups. Conclusion: With regards to the results, 5 µg/l selenium can be used in infertility clinics in order to increase sperm quality after freezing and thawing procedure.
The effects of Hydroalcolic extract of Cyperus rotundus on nociception in diabetic rat
(2014) Sima Nasri; Mohsen Naseri; Maryam Majd Barzoki
Background and Aim: Hyperalgesiua is a symptom of neuropathy due to diabetes. The present study aimed at investigating . antineurologic effect of hydroalcoholic extract of Cyperus Rotundus in diabetic rats.\nMaterials and Methods: In this experimental study, the rats were randomly divided into seven equal groups i.e. control, Cyperus Rotundus treated control (dose 100mg/kg), diabetic, diabetic receiving sodium salicylate (dose 200 mg/kg) , ., and 3 more diabetic groups peritoneally receiving . Cyperus Rotundus extract doses of 1mg/kg, 10mg/kg, 100mg/kg, respectively. The injections. were performed one week after diabetes induction for two weeks. Then, thermoalgesia rate in the subjects was assessed using formalin, acetic acid, and tail immersion of the rats in hot water.\nResults: Cyperus rotundus extract significantly reduced both phases of formalin-induced pain in a dose-dependent manner of 10mg/kg and 100mg/kg followed by a significant decrease of antineuragia (P<0.001 and P<0.01, repectively) . In the hot water tail immersion test, the treatment of the dose-dependent extract was followed by a significant increase in tail immersion latency in hot water compared to non- treated diabetic group (P<0.1 , P<0.05 and P<0.00, respectively1). In the acetic acid test. treatment with dose-dependent extract decreased the number of abdominal compressions compared to the control and non- treated diabetic groups.\nConclusion: Administration of Cyperus rotundus extract for 2 weeks .increased thermoalgia tolerance and reducedchemical pain in an experimental model of diabetes mellitus rats . Thus, this administration can act as an auxiliary treatment for diabetic hyperalgesia.