Browsing by Autor "Sinesio Torres"

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Effect of heat treatment at high temperature on in vitro seeds germination of Phaseolus vulgaris cv. ‘ICA Pijao’
(2019) Dámaris Torres; Lourdes R. García; Novisel Veitía; Amanda Martirena Ramírez; Raúl Collado; Leonardo Rivero; Sinesio Torres; Mayra Acosta-Suárez
The common bean (Phaseolus vulgaris L.) production is limited by biotic and abiotic factors. Among them, water stress, saline or high temperatures. To solve this problem, genetic improvement programs are aimed at the search for tolerant cultivars. The objective of this work was to determine the effect of heat treatment at high temperatures on the in vitro germination of P. vulgaris cv. ‘ICA Pijao’. For this, seeds of 1-4 months of harvest were used. It were treated at 35 ± 2 °C in an incubator or immersed in water in a thermal bath. As a control, seeds at room temperature (28 ± 2 °C) were used. The results showed that in vitro germination was not less than 90%, however the two heat treatment conditions affected the length and thickness of the radicle and the length of the plumule. Considering the results, the ease of application and the non-affectation of germination, it is recommended to use the heat treatment at 35 ± 2 °C in an incubator. This could be used to induce high temperature stress in the common beans cv. ‘ICA Pijao’ in an in vitro selection protocol.
In vitro response differentiation of Phaseolus vulgaris cv. ‘ICA Pijao’ and cv. ‘Tío Canela- 75’ to high temperatures
(2021) Dámaris Torres; Lourdes R. García; Sinesio Torres; Novisel Veitía; Amanda Martirena-Ramírez; Raúl Collado; Leonardo Rivero
To develop an in vitro selection protocol, it is necessary to differentiate between tolerant and susceptible cultivars to the studied factor. The present work was carried out with the objective of selecting the temperature that allows to in vitro differentiating the response of the Phaseolus vulgaris cultivars 'ICA Pijao' (susceptible) and 'Tío Canela-75' (tolerant) to high temperatures. Different temperatures (30, 35, 40, 45 and 50 °C) were applied to seeds of the two cultivars. In vitro seed germination percentage, radicle and plumule length, number of explants that formed callus and number of shoots per callus were evaluated. It was possible to differentiate between cv. ‘ICA Pijao’ (susceptible) and ‘Tío Canela- 75’ (tolerant) with the variables radicle length, plumule length, percentage of calli formed and number of regenerated shoots. It was selected 35 °C as the selective agent.